HIF1AN (NM_017902) Human Tagged ORF Clone

CAT#: RG202843

HIF1AN (tGFP-tagged) - Human hypoxia inducible factor 1, alpha subunit inhibitor (HIF1AN)

Product Images

Circular map for RG202843

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HIF1AN
• Synonyms: FIH1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202843 representing NM_017902
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGACAGCGGCGGAGGCTGTGGCCTCTGGCTCTGGAGAGCCCCGGGAGGAGGCTGGAGCCCTCG
  GCCCCGCCTGGGATGAATCCCAGTTGCGCAGTTATAGCTTCCCGACTAGGCCCATTCCGCGTCTGAGTCA
  GAGCGACCCCCGGGCAGAGGAGCTTATTGAGAATGAGGAGCCTGTGGTGCTGACCGACACAAATCTTGTG
  TATCCTGCCCTGAAATGGGACCTTGAATACCTGCAAGAGAATATTGGCAATGGAGACTTCTCTGTGTACA
  GTGCCAGCACCCACAAGTTCTTGTACTATGATGAGAAGAAGATGGCCAATTTCCAGAACTTTAAGCCGAG
  GTCCAACAGGGAAGAAATGAAATTTCATGAGTTCGTTGAGAAACTGCAGGATATACAGCAGCGAGGAGGG
  GAAGAGAGGTTGTATCTGCAGCAAACGCTCAATGACACTGTGGGCAGGAAGATTGTCATGGACTTCTTAG
  GTTTTAACTGGAACTGGATTAATAAGCAACAGGGAAAGCGTGGCTGGGGGCAGCTTACCTCTAACCTGCT
  GCTCATTGGCATGGAAGGAAATGTGACACCTGCTCACTATGATGAGCAGCAGAACTTTTTTGCTCAGATA
  AAAGGTTACAAACGATGCATCTTATTCCCTCCGGATCAGTTCGAGTGCCTCTACCCATACCCTGTTCATC
  ACCCATGTGACAGACAGAGCCAGGTGGACTTTGACAATCCCGACTACGAGAGGTTCCCTAATTTCCAAAA
  TGTGGTTGGTTACGAAACAGTGGTTGGCCCTGGTGATGTTCTTTACATCCCAATGTACTGGTGGCATCAC
  ATAGAGTCATTACTAAATGGGGGGATTACCATCACTGTGAACTTCTGGTATAAGGGGGCTCCCACCCCTA
  AGAGAATTGAATATCCTCTCAAAGCTCATCAGAAAGTGGCCATAATGAGAAACATTGAGAAGATGCTTGG
  AGAGGCCTTGGGGAACCCACAAGAGGTGGGGCCCTTGTTGAACACAATGATCAAGGGCCGATACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202843 representing NM_017902
  Red=Cloning site Green=Tags(s)
  
  MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLV
  YPALKWDLEYLQENIGNGDFSVYSASTHKFLYYDEKKMANFQNFKPRSNREEMKFHEFVEKLQDIQQRGG
  EERLYLQQTLNDTVGRKIVMDFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQI
  KGYKRCILFPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFQNVVGYETVVGPGDVLYIPMYWWHH
  IESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQEVGPLLNTMIKGRYN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017902
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017902.3
• RefSeq Size: 2964 bp
• RefSeq ORF: 1050 bp
• Locus ID: 55662
• UniProt ID: Q9NWT6
• Cytogenetics: 10q24.31
• Protein Families: Druggable Genome
• Gene Summary: Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.[UniProtKB/Swiss-Prot Function]