HSD3B7 (NM_025193) Human Tagged ORF Clone

CAT#: RG202468

  • TrueORF®

HSD3B7 (tGFP-tagged) - Human hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (HSD3B7), transcript variant 1

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro

AAV Particle: DDK


  "NM_025193" in other vectors (6)

Reconstitution Protocol

USD 657.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Mouse monoclonal turboGFP antibody, clone OTI2H8
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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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Rabbit Polyclonal Anti-HSD3B7 Antibody
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Other products for "HSD3B7"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol HSD3B7
Synonyms CBAS1; PFIC4; SDR11E3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG202468 representing NM_025193
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACTCTGCACAGGCCCAGAAGCTGGTGTACCTGGTCACAGGGGGCTGTGGCTTCCTGGGAGAGC
ACGTGGTGCGAATGCTGCTGCAGCGGGAGCCCCGGCTCGGGGAGCTGCGGGTCTTTGACCAACACCTGGG
TCCCTGGCTGGAGGAGCTGAAGACAGGGCCTGTGAGGGTGACTGCCATCCAGGGGGACGTGACCCAGGCC
CATGAGGTGGCAGCAGCTGTGGCCGGAGCCCATGTGGTCATCCACACGGCTGGGCTGGTAGACGTGTTTG
GCAGGGCCAGTCCCAAGACCATCCATGAGGTCAACGTGCAGGGTACCCGGAACGTGATCGAGGCTTGTGT
GCAGACCGGAACACGGTTCCTGGTCTACACCAGCAGCATGGAAGTTGTGGGGCCTAACACCAAAGGTCAC
CCCTTCTACAGGGGCAACGAAGACACCCCATACGAAGCAGTGCACAGGCACCCCTATCCTTGCAGCAAGG
CCCTGGCCGAGTGGCTGGTCCTGGAGGCCAACGGGAGGAAGGTCCGTGGGGGGCTGCCCCTGGTGACGTG
TGCCCTTCGTCCCACGGGCATCTACGGTGAAGGCCACCAGATCATGAGGGACTTCTACCGCCAGGGCCTG
CGCCTGGGAGGTTGGCTCTTCCGGGCCATCCCGGCCTCTGTGGAGCATGGCCGGGTCTATGTGGGCAATG
TTGCCTGGATGCACGTGCTGGCAGCCCGGGAGCTGGAGCAGCGGGCAACCCTGATGGGCGGCCAGGTATA
CTTCTGCTACGATGGATCACCCTACAGGAGCTATGAGGATTTCAACATGGAGTTCCTGGGCCCCTGCGGA
CTGCGGCTGGTGGGCGCCCGCCCATTGCTGCCCTACTGGCTGCTGGTGTTCCTGGCTGCCCTCAATGCCC
TGCTGCAGTGGCTGCTGCGGCCACTGGTGCTCTACGCACCCCTGCTGAACCCCTACACGCTGGCCGTGGC
CAACACCACCTTCACCGTCAGCACCGACAAGGCTCAGCGCCATTTCGGCTATGAGCCCCTGTTCTCGTGG
GAGGATAGCCGGACCCGTACCATTCTCTGGGTACAGGCCGCTACGGGTTCAGCCCAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG202468 representing NM_025193
Red=Cloning site Green=Tags(s)

MADSAQAQKLVYLVTGGCGFLGEHVVRMLLQREPRLGELRVFDQHLGPWLEELKTGPVRVTAIQGDVTQA
HEVAAAVAGAHVVIHTAGLVDVFGRASPKTIHEVNVQGTRNVIEACVQTGTRFLVYTSSMEVVGPNTKGH
PFYRGNEDTPYEAVHRHPYPCSKALAEWLVLEANGRKVRGGLPLVTCALRPTGIYGEGHQIMRDFYRQGL
RLGGWLFRAIPASVEHGRVYVGNVAWMHVLAARELEQRATLMGGQVYFCYDGSPYRSYEDFNMEFLGPCG
LRLVGARPLLPYWLLVFLAALNALLQWLLRPLVLYAPLLNPYTLAVANTTFTVSTDKAQRHFGYEPLFSW
EDSRTRTILWVQAATGSAQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_025193
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_025193.2, NP_079469.2
RefSeq Size 2189 bp
RefSeq ORF 1110 bp
Locus ID 80270
UniProt ID Q9H2F3
Cytogenetics 16p11.2
Domains 3Beta_HSD
Protein Families Transmembrane
Protein Pathways Metabolic pathways, Primary bile acid biosynthesis
Gene Summary This gene encodes an enzyme which is involved in the initial stages of the synthesis of bile acids from cholesterol and a member of the short-chain dehydrogenase/reductase superfamily. The encoded protein is a membrane-associated endoplasmic reticulum protein which is active against 7-alpha hydrosylated sterol substrates. Mutations in this gene are associated with a congenital bile acid synthesis defect which leads to neonatal cholestasis, a form of progressive liver disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.