PSMD3 (NM_002809) Human Tagged ORF Clone

CAT#: RG202307

PSMD3 (tGFP-tagged) - Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3)

Product Images

Circular map for RG202307

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSMD3
• Synonyms: P58; RPN3; S3; TSTA2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202307 representing NM_002809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAGGAGGGCTCGGCGCGGCGCCGCGGCGCGGACAAGGCGAAACCGCCGCCCGGCGGAGGAGAAC
  AAGAACCCCCACCGCCGCCGGCCCCCCAGGATGTGGAGATGAAAGAGGAGGCAGCGACGGGTGGCGGGTC
  AACGGGGGAGGCAGACGGCAAGACGGCGGCGGCAGCGGCTGAGCACTCCCAGCGAGAGCTGGACACAGTC
  ACCTTGGAGGACATCAAGGAGCACGTGAAACAGCTAGAGAAAGCGGTTTCAGGCAAGGAGCCGAGATTCG
  TGCTGCGGGCCCTGCGGATGCTGCCTTCCACATCACGCCGCCTCAACCACTATGTTCTGTATAAGGCTGT
  GCAGGGCTTCTTCACTTCAAATAATGCCACTCGAGACTTTTTGCTCCCCTTCCTGGAAGAGCCCATGGAC
  ACAGAGGCTGATTTACAGTTCCGTCCCCGCACGGGAAAAGCTGCGTCGAACACCCTCCTGCCTGAAGTGG
  AAGCCTATCTCCAACTCCTCGTGGTCATCTTCATGATGAACAGCAAGCGCTACAAAGAGGCACAGAAGAT
  CTCTGATGATCTGATGCAGAAGATCAGTACTCAGAACCGCCGGGCCCTAGACCTTGTAGCCGCAAAGTGT
  TACTATTATCACGCCCGGGTCTATGAGTTCCTGGACAAGCTGGATGTGGTGCGCAGCTTCTTGCATGCTC
  GGCTCCGGACAGCTACGCTTCGGCATGACGCAGACGGGCAGGCCACCCTGTTGAACCTCCTGCTGCGGAA
  TTACCTACACTACAGCTTGTACGACCAGGCTGAGAAGCTGGTGTCCAAGTCTGTGTTCCCAGAGCAGGCC
  AACAACAATGAGTGGGCCAGGTACCTCTACTACACAGGGCGAATCAAAGCCATCCAGCTGGAGTACTCAG
  TGGCCCGGAGAACGATGACCAACGCCCTTCGCAAGGCCCCTCAGCACACAGCTGTCGGCTTCAAACAGAC
  GGTGCACAAGCTTCTCATCGTGGTGGAGCTGTTGCTGGGGGAGATCCCTGACCGGCTGCAGTTCCGCCAG
  CCCTCCCTCAAGCGCTCACTCATGCCCTATTTCCTTCTGACTCAAGCTGTCAGGACAGGAAACCTAGCCA
  AGTTCAACCAGGTCCTGGATCAGTTTGGGGAGAAGTTTCAAGCAGATGGGACCTACACCCTAATTATCCG
  GCTGCGGCACAACGTGATTAAGACAGGTGTACGCATGATCAGCCTCTCCTATTCCCGAATCTCCTTGGCT
  GACATCGCCCAGAAGCTGCAGTTGGATAGCCCCGAAGATGCAGAGTTCATTGTTGCCAAGGCCATCCGGG
  ATGGTGTCATTGAGGCCAGCATCAACCACGAGAAGGGCTATGTCCAATCCAAGGAGATGATTGACATCTA
  TTCCACCCGAGAGCCCCAGCTAGCCTTCCACCAGCGCATCTCCTTCTGCCTAGATATCCACAACATGTCT
  GTCAAGGCCATGAGGTTTCCTCCCAAATCGTACAACAAGGACTTGGAGTCTGCAGAGGAACGGCGTGAGC
  GAGAACAGCAGGACTTGGAGTTTGCCAAGGAGATGGCAGAAGATGATGATGACAGCTTCCCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202307 representing NM_002809
  Red=Cloning site Green=Tags(s)
  
  MKQEGSARRRGADKAKPPPGGGEQEPPPPPAPQDVEMKEEAATGGGSTGEADGKTAAAAAEHSQRELDTV
  TLEDIKEHVKQLEKAVSGKEPRFVLRALRMLPSTSRRLNHYVLYKAVQGFFTSNNATRDFLLPFLEEPMD
  TEADLQFRPRTGKAASNTLLPEVEAYLQLLVVIFMMNSKRYKEAQKISDDLMQKISTQNRRALDLVAAKC
  YYYHARVYEFLDKLDVVRSFLHARLRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKSVFPEQA
  NNNEWARYLYYTGRIKAIQLEYSVARRTMTNALRKAPQHTAVGFKQTVHKLLIVVELLLGEIPDRLQFRQ
  PSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQADGTYTLIIRLRHNVIKTGVRMISLSYSRISLA
  DIAQKLQLDSPEDAEFIVAKAIRDGVIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMS
  VKAMRFPPKSYNKDLESAEERREREQQDLEFAKEMAEDDDDSFP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002809
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002809.2, NP_002800.2
• RefSeq Size: 2174 bp
• RefSeq ORF: 1605 bp
• Locus ID: 5709
• UniProt ID: O43242
• Cytogenetics: 17q21.1
• Domains: PCI
• Protein Pathways: Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the proteasome subunit S3 family that functions as one of the non-ATPase subunits of the 19S regulator lid. Single nucleotide polymorphisms in this gene are associated with neutrophil count. [provided by RefSeq, Jul 2012]