ARHGEF19 (NM_153213) Human Tagged ORF Clone

CAT#: RG202273

ARHGEF19 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 19 (ARHGEF19)

Product Images

Circular map for RG202273

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ARHGEF19
• Synonyms: WGEF
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202273 representing NM_153213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGTGGGCCACCTGCTACCCTCCAGCCCCACCTGACTGGGCCACCTGGCACTGCCCACCACCCTG
  TAGCAGTGTGCCAGCAGGAGAGTCTGTCCTTTGCAGAGCTGCCCGCCCTGAAGCCCCCGAGCCCAGTGTG
  TCTGGACCTTTTCCCTGTTGCCCCAGAGGAGCTTCGGGCTCCTGGCAGCCGCTGGTCCCTGGGGACCCCT
  GCCCCTCTCCAAGGGTTGCTATGGCCATTATCCCCAGGAGGCTCAGATACAGAGATCACCAGCGGGGGGA
  TGCGGCCCAGCAGGGCTGGCAGCTGGCCACACTGTCCTGGTGCCCAGCCCCCAGCTCTGGAGGGACCCTG
  GAGTCCCCGACACACACAGCCACAGCGCCGGGCCAGCCACGGCTCGGAGAAGAAGTCTGCCTGGCGCAAG
  ATGCGGGTGTACCAGCGTGAAGAGGTCCCCGGCTGCCCCGAGGCCCACGCTGTCTTCCTAGAGCCTGGCC
  AGGTAGTGCAAGAGCAGGCCCTGAGCACAGAGGAGCCCAGGGTGGAGTTGTCTGGGTCCACCCGAGTGAG
  CCTCGAAGGTCCTGAGCGGAGGCGCTTCTCGGCATCGGAGCTGATGACCCGGCTGCACTCTTCTCTGCGC
  CTGGGGCGGAATTCAGCAGCCCGGGCACTCATCTCTGGGTCAGGCACCGGAGCAGCCCGGGAAGGGAAAG
  CATCTGGAATGGAGGCTCGAAGTGTAGAGATGAGCGGGGACCGGGTGTCGCGGCCAGCCCCTGGTGACTC
  ACGAGAGGGCGATTGGTCCGAGCCCAGGCTAGACACACAGGAAGAGCCGCCTTTGGGGTCCAGGAGCACC
  AACGAGCGGCGCCAGTCTCGATTCCTCCTTAACTCCGTCCTCTATCAGGAATACAGCGACGTGGCCAGCG
  CCCGCGAACTGCGGCGGCAGCAGCGCGAGGAGGAGGGCCCGGGGGACGAGGCCGAGGGCGCAGAGGAGGG
  GCCGGGGCCGCCGCGGGCCAACCTCTCCCCCAGCAGCTCCTTCCGGGCGCAGCGCTCGGCGCGAGGCTCC
  ACCTTCTCGCTGTGGCAGGATATCCCCGACGTACGCGGCAGCGGCGTCCTGGCCACGCTGAGCCTGCGGG
  ACTGCAAGCTGCAGGAGGCCAAGTTTGAGCTGATCACCTCCGAGGCCTCCTACATCCACAGCCTGTCGGT
  GGCTGTGGGCCACTTCTTAGGCTCTGCCGAGCTGAGCGAGTGTCTGGGGGCGCAGGACAAGCAGTGGCTG
  TTTTCCAAACTGCCCGAGGTCAAGAGCACCAGCGAGAGGTTCCTGCAGGACCTGGAGCAGCGGCTGGAGG
  CAGATGTGCTGCGCTTCAGCGTGTGCGACGTGGTGCTGGACCACTGCCCGGCCTTCCGCAGAGTCTACCT
  GCCCTATGTCACCAACCAGGCCTACCAGGAGCGCACCTACCAGCGCCTGCTCCTGGAGAACCCCAGGTTC
  CCTGGCATCCTGGCTCGCCTGGAGGAGTCTCCTGTGTGCCAGCGTCTGCCCCTTACCTCCTTCCTTATCC
  TGCCCTTCCAGAGGATCACCCGCCTCAAGATGTTGGTGGAGAACATCCTGAAGCGGACAGCACAGGGCTC
  TGAAGACGAAGACATGGCCACCAAGGCCTTCAATGCGCTCAAGGAGCTGGTGCAGGAGTGCAATGCTAGT
  GTACAGTCCATGAAGAGGACAGAGGAACTCATCCACCTGAGCAAGAAGATCCACTTTGAGGGCAAGATTT
  TCCCGCTGATCTCTCAGGCCCGCTGGCTGGTTCGGCATGGAGAGTTGGTAGAGCTGGCACCACTGCCTGC
  AGCACCCCCTGCCAAGCTGAAGCTGTCCAGCAAGGCAGTCTACCTCCACCTCTTCAATGACTGCTTGCTG
  CTCTCTCGGCGGAAGGAGCTAGGGAAGTTTGCCGTTTTCGTCCATGCCAAGATGGCTGAGCTGCAGGTGC
  GGGACCTGAGCCTGAAGCTGCAGGGCATCCCCGGCCACGTGTTCCTCCTCCAGCTCCTCCACGGGCAGCA
  CATGAAGCACCAGTTCCTGCTGCGGGCCCGGACGGAAAGTGAGAAGCAGCGATGGATCTCAGCCTTGTGC
  CCCTCCAGCCCCCAGGAGGACAAGGAGGTCATCAGTGAGGGGGAAGATTGCCCCCAGGTTCAGTGTGTTA
  GGACATACAAGGCACTGCACCCAGATGAGCTGACCTTGGAGAAGACTGACATCCTGTCAGTGAGGACCTG
  GACCAGTGACGGCTGGCTGGAAGGGGTCCGCCTGGCAGATGGTGAGAAGGGGTGGGTGCCCCAGGCCTAT
  GTGGAAGAGATCAGCAGCCTCAGCGCCCGCCTCCGAAACCTCCGGGAGAATAAGCGAGTCACAAGTGCCA
  CCAGCAAACTGGGGGAGGCTCCTGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202273 representing NM_153213
  Red=Cloning site Green=Tags(s)
  
  MDCGPPATLQPHLTGPPGTAHHPVAVCQQESLSFAELPALKPPSPVCLDLFPVAPEELRAPGSRWSLGTP
  APLQGLLWPLSPGGSDTEITSGGMRPSRAGSWPHCPGAQPPALEGPWSPRHTQPQRRASHGSEKKSAWRK
  MRVYQREEVPGCPEAHAVFLEPGQVVQEQALSTEEPRVELSGSTRVSLEGPERRRFSASELMTRLHSSLR
  LGRNSAARALISGSGTGAAREGKASGMEARSVEMSGDRVSRPAPGDSREGDWSEPRLDTQEEPPLGSRST
  NERRQSRFLLNSVLYQEYSDVASARELRRQQREEEGPGDEAEGAEEGPGPPRANLSPSSSFRAQRSARGS
  TFSLWQDIPDVRGSGVLATLSLRDCKLQEAKFELITSEASYIHSLSVAVGHFLGSAELSECLGAQDKQWL
  FSKLPEVKSTSERFLQDLEQRLEADVLRFSVCDVVLDHCPAFRRVYLPYVTNQAYQERTYQRLLLENPRF
  PGILARLEESPVCQRLPLTSFLILPFQRITRLKMLVENILKRTAQGSEDEDMATKAFNALKELVQECNAS
  VQSMKRTEELIHLSKKIHFEGKIFPLISQARWLVRHGELVELAPLPAAPPAKLKLSSKAVYLHLFNDCLL
  LSRRKELGKFAVFVHAKMAELQVRDLSLKLQGIPGHVFLLQLLHGQHMKHQFLLRARTESEKQRWISALC
  PSSPQEDKEVISEGEDCPQVQCVRTYKALHPDELTLEKTDILSVRTWTSDGWLEGVRLADGEKGWVPQAY
  VEEISSLSARLRNLRENKRVTSATSKLGEAPV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153213
• ORF Size: 2406 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153213.5
• RefSeq Size: 3045 bp
• RefSeq ORF: 2409 bp
• Locus ID: 128272
• UniProt ID: Q8IW93
• Cytogenetics: 1p36.13
• Gene Summary: Guanine nucleotide exchange factors (GEFs) such as ARHGEF19 accelerate the GTPase activity of Rho GTPases (see RHOA, MIM 165390).[supplied by OMIM, Dec 2008]