GTF2IRD1 (NM_016328) Human Tagged ORF Clone

CAT#: RG202128

GTF2IRD1 (tGFP-tagged) - Human GTF2I repeat domain containing 1 (GTF2IRD1), transcript variant 1

Product Images

Circular map for RG202128

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GTF2IRD1
• Synonyms: BEN; CREAM1; GTF3; hMusTRD1alpha1; MUSTRD1; RBAP2; WBS; WBSCR11; WBSCR12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202128 representing NM_016328
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTTGCTGGGTAAGCGCTGTGACGTCCCCACCAACGGCTGCGGACCCGACCGCTGGAACTCCGCGT
  TCACCCGCAAAGACGAGATCATCACCAGCCTCGTGTCTGCCTTAGACTCCATGTGCTCAGCGCTGTCCAA
  ACTGAACGCCGAGGTGGCCTGTGTCGCCGTGCACGATGAGAGCGCCTTTGTGGTGGGCACAGAGAAGGGG
  AGAATGTTCCTGAATGCCCGGAAGGAGCTACAGTCAGACTTCCTCAGGTTCTGCCGAGGGCCCCCGTGGA
  AGGATCCGGAGGCAGAGCACCCCAAGAAGGTGCAGCGGGGCGAGGGTGGAGGCCGTAGCCTCCCTCGGTC
  CTCCCTGGAACATGGCTCAGATGTGTACCTTCTGCGGAAGATGGTAGAGGAGGTGTTTGATGTTCTTTAT
  AGCGAGGCCCTGGGAAGGGCCAGTGTGGTGCCACTGCCCTATGAGAGGCTGCTCAGGGAGCCAGGGCTGC
  TGGCCGTGCAGGGGCTGCCCGAGGGCCTGGCCTTCCGAAGGCCAGCCGAGTATGACCCCAAGGCCCTCAT
  GGCCATCCTGGAACACAGCCACCGCATCCGCTTCAAGCTCAAGAGGCCACTTGAGGATGGCGGGCGGGAC
  TCGAAGGCCCTGGTGGAGCTGAACGGTGTCTCCCTGATTCCCAAGGGGTCACGGGACTGTGGCCTGCATG
  GCCAGGCCCCCAAGGTGCCACCCCAGGACCTGCCCCCAACCGCCACCTCCTCCTCCATGGCCAGCTTCCT
  GTACAGCACGGCGCTCCCCAACCACGCCATCCGAGAGCTCAAGCAGGAAGCACCTTCCTGCCCCCTTGCC
  CCCAGCGACCTGGGCCTGAGTCGGCCCATGCCAGAGCCCAAGGCCACCGGTGCCCAAGACTTCTCCGACT
  GTTGTGGACAGAAGCCCACTGGGCCTGGTGGGCCTCTCATCCAGAACGTCCATGCCTCCAAGCGCATTCT
  CTTCTCCATCGTCCATGACAAGTCAGAGAAGTGGGACGCCTTCATAAAGGAAACCGAGGACATCAACACG
  CTCCGGGAGTGTGTGCAGATCCTGTTTAACAGCAGATATGCGGAAGCCCTGGGCCTGGACCACATGGTCC
  CCGTGCCCTACCGGAAGATTGCCTGTGACCCGGAGGCTGTGGAGATCGTGGGCATCCCGGACAAGATCCC
  CTTCAAGCGCCCCTGCACTTACGGAGTCCCCAAGCTGAAGCGGATCCTGGAGGAGCGCCATAGTATCCAC
  TTCATCATTAAGAGGATGTTTGATGAGCGAATTTTCACAGGGAACAAGTTTACCAAAGACACCACGAAGC
  TGGAGCCAGCCAGCCCGCCAGAGGACACCTCTGCAGAGGTCTCTAGGGCCACCGTCCTTGACCTTGCTGG
  GAATGCTCGGTCAGACAAGGGCAGCATGTCTGAAGACTGTGGGCCAGGAACCTCCGGGGAGCTGGGCGGG
  CTGAGGCCGATCAAAATTGAGCCAGAGGATCTGGACATCATTCAGGTCACCGTCCCAGACCCCTCGCCAA
  CCTCTGAGGAAATGACAGACTCGATGCCTGGGCACCTGCCATCGGAGGATTCTGGTTATGGGATGGAGAT
  GCTGACAGACAAAGGTCTGAGTGAGGACGCGCGGCCCGAGGAGAGGCCCGTGGAGGACAGCCACGGTGAC
  GTGATCCGGCCCCTGCGGAAGCAGGTGGAGCTGCTCTTCAACACACGATACGCCAAGGCCATTGGCATCT
  CGGAGCCCGTCAAGGTGCCGTACTCCAAGTTTCTGATGCACCCGGAGGAGCTGTTTGTGGTGGGACTGCC
  TGAAGGCATCTCCCTCCGCAGGCCCAACTGCTTCGGGATCGCCAAGCTCCGGAAGATTCTGGAGGCCAGC
  AACAGCATCCAGTTTGTCATCAAGAGGCCCGAGCTGCTCACTGAGGGAGTCAAAGAGCCCATCGTGGATA
  GTCAAGGAACTGCCTCCTCACTTGGCTTCTCTCCCCCTGCCCTGCCCCCAGAGAGGGATTCCGGGGACCC
  TCTGGTGGACGAGAGCCTGAAGAGACAGGGCTTTCAAGAAAATTATGACGCGAGGCTCTCACGGATCGAC
  ATCGCCAACACACTAAGGGAGCAGGTCCAGGACCTTTTCAATAAGAAATACGGGGAAGCCTTGGGCATCA
  AGTACCCGGTCCAGGTCCCCTACAAGCGGATCAAGAGTAACCCCGGCTCCGTGATCATCGAGGGGCTGCC
  CCCAGGAATCCCGTTCCGAAAGCCCTGTACCTTCGGCTCCCAGAACCTGGAGAGGATTCTTGCTGTGGCT
  GACAAGATCAAGTTCACAGTCACCAGGCCTTTCCAAGGACTCATCCCAAAGCCTGATGAAGATGACGCCA
  ACAGACTCGGGGAGAAGGTGATCCTGCGGGAGCAGGTGAAGGAACTCTTCAACGAGAAATACGGTGAGGC
  CCTGGGCCTGAACCGGCCGGTGCTGGTCCCTTATAAACTAATCCGGGACAGCCCAGACGCCGTGGAGGTC
  ACGGGTCTGCCTGATGACATCCCCTTCCGGAACCCCAACACGTACGACATCCACCGGCTGGAGAAGATCC
  TGAAGGCCCGAGAGCATGTCCGCATGGTCATCATTAACCAGCTCCAACCCTTTGCAGAAATCTGCAATGA
  TGCCAAGGTGCCAGCCAAAGACAGCAGCATTCCCAAGCGCAAGAGAAAGCGGGTCTCGGAAGGAAATTCC
  GTCTCCTCTTCCTCCTCGTCTTCCTCTTCCTCGTCCTCTAACCCGGATTCAGTGGCATCGGCCAACCAGA
  TCTCACTCGTGCAATGGCCAATGTACATGGTGGACTATGCCGGCCTGAACGTGCAGCTCCCGGGACCTCT
  TAATTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202128 representing NM_016328
  Red=Cloning site Green=Tags(s)
  
  MALLGKRCDVPTNGCGPDRWNSAFTRKDEIITSLVSALDSMCSALSKLNAEVACVAVHDESAFVVGTEKG
  RMFLNARKELQSDFLRFCRGPPWKDPEAEHPKKVQRGEGGGRSLPRSSLEHGSDVYLLRKMVEEVFDVLY
  SEALGRASVVPLPYERLLREPGLLAVQGLPEGLAFRRPAEYDPKALMAILEHSHRIRFKLKRPLEDGGRD
  SKALVELNGVSLIPKGSRDCGLHGQAPKVPPQDLPPTATSSSMASFLYSTALPNHAIRELKQEAPSCPLA
  PSDLGLSRPMPEPKATGAQDFSDCCGQKPTGPGGPLIQNVHASKRILFSIVHDKSEKWDAFIKETEDINT
  LRECVQILFNSRYAEALGLDHMVPVPYRKIACDPEAVEIVGIPDKIPFKRPCTYGVPKLKRILEERHSIH
  FIIKRMFDERIFTGNKFTKDTTKLEPASPPEDTSAEVSRATVLDLAGNARSDKGSMSEDCGPGTSGELGG
  LRPIKIEPEDLDIIQVTVPDPSPTSEEMTDSMPGHLPSEDSGYGMEMLTDKGLSEDARPEERPVEDSHGD
  VIRPLRKQVELLFNTRYAKAIGISEPVKVPYSKFLMHPEELFVVGLPEGISLRRPNCFGIAKLRKILEAS
  NSIQFVIKRPELLTEGVKEPIVDSQGTASSLGFSPPALPPERDSGDPLVDESLKRQGFQENYDARLSRID
  IANTLREQVQDLFNKKYGEALGIKYPVQVPYKRIKSNPGSVIIEGLPPGIPFRKPCTFGSQNLERILAVA
  DKIKFTVTRPFQGLIPKPDEDDANRLGEKVILREQVKELFNEKYGEALGLNRPVLVPYKLIRDSPDAVEV
  TGLPDDIPFRNPNTYDIHRLEKILKAREHVRMVIINQLQPFAEICNDAKVPAKDSSIPKRKRKRVSEGNS
  VSSSSSSSSSSSSNPDSVASANQISLVQWPMYMVDYAGLNVQLPGPLNY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016328
• ORF Size: 2877 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016328.1, NP_057412.1
• RefSeq Size: 3446 bp
• RefSeq ORF: 2880 bp
• Locus ID: 9569
• UniProt ID: Q9UHL9
• Cytogenetics: 7q11.23
• Domains: GTF2I
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: The protein encoded by this gene contains five GTF2I-like repeats and each repeat possesses a potential helix-loop-helix (HLH) motif. It may have the ability to interact with other HLH-proteins and function as a transcription factor or as a positive transcriptional regulator under the control of Retinoblastoma protein. This gene plays a role in craniofacial and cognitive development and mutations have been associated with Williams-Beuren syndrome, a multisystem developmental disorder caused by deletion of multiple genes at 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]