FA2H (NM_024306) Human Tagged ORF Clone

CAT#: RG202093

FA2H (tGFP-tagged) - Human fatty acid 2-hydroxylase (FA2H)

Product Images

Circular map for RG202093

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FA2H
• Synonyms: FAAH; FAH1; FAXDC1; SCS7; SPG35
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG202093 representing NM_024306
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCCGCTCCGCCCCCCGCCGCCTCCTTCTCGCCCTCCGAGGTCCAGCGGCGCCTGGCGGCCGGCG
  CGTGCTGGGTCCGCCGCGGGGCCCGCCTCTACGACCTCTCCAGCTTCGTGCGGCACCACCCGGGGGGCGA
  GCAGCTGCTGCGGGCCAGGGCGGGCCAGGACATCAGCGCCGACCTGGACGGGCCGCCGCACAGGCACTCG
  GCCAACGCGCGCCGCTGGCTGGAGCAGTACTACGTGGGAGAGCTCCGCGGGGAGCAGCAGGGCTCCATGG
  AGAACGAGCCTGTAGCCCTTGAGGAAACTCAGAAGACAGATCCTGCTATGGAACCACGGTTCAAAGTGGT
  GGATTGGGACAAGGACCTGGTGGACTGGCGAAAGCCTCTCCTGTGGCAGGTGGGCCACTTGGGAGAGAAG
  TACGATGAGTGGGTTCACCAGCCGGTGACCAGGCCCATCCGCCTCTTCCACTCAGACCTCATTGAGGGCC
  TCTCTAAGACTGTCTGGTACAGTGTCCCCATCATCTGGGTGCCCCTGGTGCTGTATCTCAGCTGGTCCTA
  CTACCGAACCTTTGCCCAGGGCAACGTCCGACTCTTCACGTCATTTACAACAGAGTACACGGTGGCAGTG
  CCCAAGTCCATGTTCCCCGGGCTCTTCATGCTGGGGACATTCCTCTGGAGCCTCATCGAGTACCTCATCC
  ACCGCTTCCTGTTCCACATGAAGCCCCCCAGCGACAGCTATTACCTCATCATGCTGCACTTCGTCATGCA
  CGGCCAGCACCACAAGGCACCCTTCGACGGCTCCCGCCTGGTCTTCCCCCCTGTGCCAGCCTCCCTGGTG
  ATCGGCGTCTTCTACTTGTGCATGCAGCTCATCCTGCCCGAGGCAGTAGGGGGCACTGTGTTTGCGGGGG
  GCCTCCTGGGCTACGTCCTCTATGACATGACCCATTACTACCTGCACTTTGGCTCGCCGCACAAGGGCTC
  CTACCTGTACAGCCTGAAGGCCCACCACGTCAAGCACCACTTTGCACATCAGAAGTCAGGATTTGGTATC
  AGCACTAAATTGTGGGATTACTGTTTCCACACCCTCACTCCAGAGAAACCCCACCTGAAGACGCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG202093 representing NM_024306
  Red=Cloning site Green=Tags(s)
  
  MAPAPPPAASFSPSEVQRRLAAGACWVRRGARLYDLSSFVRHHPGGEQLLRARAGQDISADLDGPPHRHS
  ANARRWLEQYYVGELRGEQQGSMENEPVALEETQKTDPAMEPRFKVVDWDKDLVDWRKPLLWQVGHLGEK
  YDEWVHQPVTRPIRLFHSDLIEGLSKTVWYSVPIIWVPLVLYLSWSYYRTFAQGNVRLFTSFTTEYTVAV
  PKSMFPGLFMLGTFLWSLIEYLIHRFLFHMKPPSDSYYLIMLHFVMHGQHHKAPFDGSRLVFPPVPASLV
  IGVFYLCMQLILPEAVGGTVFAGGLLGYVLYDMTHYYLHFGSPHKGSYLYSLKAHHVKHHFAHQKSGFGI
  STKLWDYCFHTLTPEKPHLKTQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024306
• ORF Size: 1116 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024306.5
• RefSeq Size: 2419 bp
• RefSeq ORF: 1119 bp
• Locus ID: 79152
• UniProt ID: Q7L5A8
• Cytogenetics: 16q23.1
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein that catalyzes the synthesis of 2-hydroxysphingolipids, a subset of sphingolipids that contain 2-hydroxy fatty acids. Sphingolipids play roles in many cellular processes and their structural diversity arises from modification of the hydrophobic ceramide moiety, such as by 2-hydroxylation of the N-acyl chain, and the existence of many different head groups. Mutations in this gene have been associated with leukodystrophy dysmyelinating with spastic paraparesis with or without dystonia.[provided by RefSeq, Mar 2010]