RALB (NM_002881) Human Tagged ORF Clone

CAT#: RG201982

RALB (tGFP-tagged) - Human v-ral simian leukemia viral oncogene homolog B (ras related; GTP binding protein) (RALB)

Product Images

Circular map for RG201982

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RALB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201982 representing NM_002881
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCAACAAGAGTAAGGGCCAGAGCTCCTTGGCCCTCCACAAGGTGATCATGGTTGGCAGCGGAG
  GCGTTGGCAAGTCAGCCCTGACGCTTCAGTTCATGTATGACGAGTTTGTAGAAGACTATGAACCTACCAA
  AGCTGACAGTTATAGAAAGAAAGTGGTTCTTGATGGGGAAGAAGTTCAGATAGATATTCTGGACACCGCT
  GGGCAAGAGGACTACGCAGCCATTCGAGATAACTACTTTCGGAGTGGGGAAGGGTTTCTTCTTGTGTTCT
  CAATCACAGAACATGAATCCTTTACAGCAACTGCCGAATTCAGGGAACAGATTCTCCGTGTGAAGGCTGA
  AGAAGATAAAATTCCACTGCTCGTCGTGGGAAACAAGTCTGACCTAGAGGAGCGGAGGCAGGTGCCTGTG
  GAGGAGGCCAGGAGTAAAGCCGAAGAGTGGGGCGTGCAGTACGTGGAGACGTCAGCGAAGACCCGGGCCA
  ACGTGGACAAGGTGTTCTTTGACCTAATGAGAGAAATCAGAACAAAGAAGATGTCAGAAAACAAAGACAA
  GAATGGCAAGAAAAGCAGCAAGAACAAGAAAAGTTTTAAAGAAAGATGTTGCTTACTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201982 representing NM_002881
  Red=Cloning site Green=Tags(s)
  
  MAANKSKGQSSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTA
  GQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPV
  EEARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRTKKMSENKDKNGKKSSKNKKSFKERCCLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002881
• ORF Size: 618 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002881.3
• RefSeq Size: 2275 bp
• RefSeq ORF: 621 bp
• Locus ID: 5899
• UniProt ID: P11234
• Cytogenetics: 2q14.2
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Pancreatic cancer, Pathways in cancer
• Gene Summary: This gene encodes a GTP-binding protein that belongs to the small GTPase superfamily and Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. [provided by RefSeq, Jul 2008]