SNX1 (NM_003099) Human Tagged ORF Clone

CAT#: RG201844

SNX1 (tGFP-tagged) - Human sorting nexin 1 (SNX1), transcript variant 1

Product Images

Circular map for RG201844

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SNX1
• Synonyms: HsT17379; VPS5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201844 representing NM_003099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGGTGGTGGTGGCTGTAGCGCTTCGGAGAGACTGCCTCCGCCCTTCCCCGGCCTGGAGCCGG
  AGTCCGAGGGGGCGGCCGGGGGATCAGAACCCGAGGCTGGGGACAGCGACACCGAGGGGGAGGACATTTT
  CACCGGCGCCGCGGTGGTCAGTAAACATCAGTCTCCAAAGATAACTACATCCCTTCTTCCCATCAACAAT
  GGCTCCAAAGAAAATGGGATCCATGAAGAACAAGACCAAGAGCCACAGGATCTCTTTGCAGATGCCACAG
  TGGAGCTATCCTTGGACAGCACACAAAATAATCAGAAGAAGGTGCTAGCCAAAACACTCATTTCTCTTCC
  TCCTCAGGAAGCCACAAATTCTTCGAAGCCCCAGCCAACCTATGAGGAGCTAGAGGAAGAAGAACAGGAG
  GATCAATTTGATTTGACAGTCGGTATAACTGATCCTGAGAAGATAGGGGATGGTATGAATGCATATGTAG
  CCTACAAAGTTACAACACAGACAAGCTTACCATTGTTCAGAAGCAAACAGTTTGCAGTAAAAAGAAGATT
  TAGTGACTTTCTGGGTCTTTATGAGAAGCTTTCCGAGAAGCACTCTCAGAATGGCTTCATTGTCCCTCCA
  CCCCCGGAGAAGAGCCTCATAGGGATGACAAAAGTGAAAGTTGGGAAGGAAGATTCTTCTTCTGCAGAAT
  TTCTTGAAAAACGGAGGGCCGCTTTAGAAAGGTACCTTCAGAGGATTGTAAATCATCCTACCATGTTACA
  GGACCCTGACGTCAGAGAGTTCTTGGAAAAAGAAGAGCTGCCACGTGCCGTGGGTACCCAGACATTGAGT
  GGTGCTGGTCTCCTCAAGATGTTCAACAAAGCCACAGATGCCGTCAGCAAAATGACCATCAAGATGAATG
  AATCAGACATTTGGTTTGAGGAGAAGCTCCAGGAGGTAGAGTGTGAGGAGCAGCGCTTACGGAAACTGCA
  TGCTGTTGTAGAAACTCTAGTCAACCATAGGAAAGAGCTAGCGCTGAACACAGCCCAGTTTGCAAAGAGT
  CTAGCCATGCTTGGGAGCTCTGAGGACAACACGGCATTGTCACGGGCACTCTCCCAGCTGGCTGAGGTGG
  AAGAAAAAATTGAGCAGCTCCACCAGGAACAGGCCAACAATGACTTCTTCCTCCTTGCTGAGCTCCTGAG
  TGACTACATTCGCCTCCTGGCCATAGTCCGCGCTGCCTTCGACCAGCGCATGAAGACATGGCAGCGCTGG
  CAGGATGCCCAAGCCACACTGCAGAAGAAGCGGGAGGCCGAGGCTCGGCTGCTGTGGGCCAACAAGCCTG
  ATAAGCTGCAGCAGGCCAAGGACGAGATCCTCGAGTGGGAGTCTCGGGTGACTCAATATGAAAGGGACTT
  CGAGAGGATTTCAACAGTGGTCCGAAAAGAAGTGATACGGTTTGAGAAAGAGAAATCCAAGGACTTCAAG
  AACCACGTGATCAAGTACCTTGAGACACTCCTTTACTCACAGCAGCAGCTGGCAAAGTACTGGGAAGCCT
  TCCTTCCTGAGGCAAAGGCCATCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201844 representing NM_003099
  Red=Cloning site Green=Tags(s)
  
  MASGGGGCSASERLPPPFPGLEPESEGAAGGSEPEAGDSDTEGEDIFTGAAVVSKHQSPKITTSLLPINN
  GSKENGIHEEQDQEPQDLFADATVELSLDSTQNNQKKVLAKTLISLPPQEATNSSKPQPTYEELEEEEQE
  DQFDLTVGITDPEKIGDGMNAYVAYKVTTQTSLPLFRSKQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPP
  PPEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVGTQTLS
  GAGLLKMFNKATDAVSKMTIKMNESDIWFEEKLQEVECEEQRLRKLHAVVETLVNHRKELALNTAQFAKS
  LAMLGSSEDNTALSRALSQLAEVEEKIEQLHQEQANNDFFLLAELLSDYIRLLAIVRAAFDQRMKTWQRW
  QDAQATLQKKREAEARLLWANKPDKLQQAKDEILEWESRVTQYERDFERISTVVRKEVIRFEKEKSKDFK
  NHVIKYLETLLYSQQQLAKYWEAFLPEAKAIS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003099
• ORF Size: 1566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003099.3, NP_003090.2
• RefSeq Size: 8221 bp
• RefSeq ORF: 1569 bp
• Locus ID: 6642
• UniProt ID: Q13596
• Cytogenetics: 15q22.31
• Domains: PX, Sorting_nexin
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]