Aspartate Aminotransferase (GOT1) (NM_002079) Human Tagged ORF Clone

CAT#: RG201757

GOT1 (tGFP-tagged) - Human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)

Product Images

Circular map for RG201757

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aspartate Aminotransferase
• Synonyms: AST1; ASTQTL1; cAspAT; cCAT; GIG18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201757 representing NM_002079
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTCCGTCAGTCTTTGCCGAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAGCTCACTGCCG
  ACTTCAGGGAGGATCCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCATATCGCACGGATGACTGCCA
  TCCCTGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAGATTGCTAATGACAATAGCCTAAATCACGAG
  TATCTGCCAATCCTGGGCCTGGCTGAGTTCCGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGATGACAGCC
  CAGCACTCAAGGAGAAGCGGGTAGGAGGTGTGCAATCTTTGGGGGGAACAGGTGCACTTCGAATTGGAGC
  TGATTTCTTAGCGCGTTGGTACAATGGAACAAACAACAAGAACACACCTGTCTATGTGTCCTCACCAACC
  TGGGAGAATCACAATGCTGTGTTTTCCGCTGCTGGTTTTAAAGACATTCGGTCCTATCGCTACTGGGATG
  CAGAGAAGAGAGGATTGGACCTCCAGGGCTTCCTGAATGATCTGGAGAATGCTCCTGAGTTCTCCATTGT
  TGTCCTCCACGCCTGTGCACACAACCCAACTGGGATTGACCCAACTCCGGAGCAGTGGAAGCAGATTGCT
  TCTGTCATGAAGCACCGGTTTCTGTTCCCCTTCTTTGACTCAGCCTATCAGGGCTTCGCATCTGGAAACC
  TGGAGAGAGATGCCTGGGCCATTCGCTATTTTGTGTCTGAAGGCTTCGAGTTCTTCTGTGCCCAGTCCTT
  CTCCAAGAACTTCGGGCTCTACAATGAGAGAGTCGGGAATCTGACTGTGGTTGGAAAAGAACCTGAGAGC
  ATCCTGCAAGTCCTTTCCCAGATGGAGAAGATCGTGCGGATTACTTGGTCCAATCCCCCCGCCCAGGGAG
  CACGAATTGTGGCCAGCACCCTCTCTAACCCTGAGCTCTTTGAGGAATGGACAGGTAATGTGAAGACAAT
  GGCTGACCGGATTCTGACCATGAGATCTGAACTCAGGGCACGACTAGAAGCCCTCAAAACCCCTGGGACC
  TGGAACCACATCACTGATCAAATTGGCATGTTCAGCTTCACTGGGTTGAACCCCAAGCAGGTTGAGTATC
  TGGTCAATGAAAAGCACATCTACCTGCTGCCAAGTGGTCGAATCAACGTGAGTGGCTTAACCACCAAAAA
  TCTAGATTACGTGGCCACCTCCATCCATGAAGCAGTCACCAAAATCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201757 representing NM_002079
  Red=Cloning site Green=Tags(s)
  
  MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHE
  YLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPT
  WENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIA
  SVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPES
  ILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGT
  WNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002079
• ORF Size: 1239 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002079.3
• RefSeq Size: 1941 bp
• RefSeq ORF: 1242 bp
• Locus ID: 2805
• UniProt ID: P17174
• Cytogenetics: 10q24.2
• Domains: aminotran_1_2
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism
• Gene Summary: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]