LENG4 (MBOAT7) (NM_024298) Human Tagged ORF Clone

CAT#: RG201320

MBOAT7 (tGFP-tagged) - Human membrane bound O-acyltransferase domain containing 7 (MBOAT7), transcript variant 1

Product Images

Circular map for RG201320

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LENG4
• Synonyms: BB1; hMBOA-7; LENG4; LPIAT; LPLAT; LRC4; MBOA7; MRT57; OACT7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201320 representing NM_024298
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCTGAAGAATGGACGTATCTAGTGGTTCTTCTTATCTCCATCCCCATCGGCTTCCTCTTTAAGA
  AAGCCGGTCCTGGGCTGAAGAGATGGGGAGCAGCCGCTGTGGGCCTGGGGCTCACCCTGTTCACCTGTGG
  CCCCCACACTTTGCATTCTCTGGTCACCATCCTCGGGACCTGGGCCCTCATTCAGGCCCAGCCCTGCTCC
  TGCCACGCCCTGGCTCTGGCCTGGACTTTCTCCTATCTCCTGTTCTTCCGAGCCCTCAGCCTCCTGGGCC
  TGCCCACTCCCACGCCCTTCACCAATGCCGTCCAGCTGCTGCTGACGCTGAAGCTGGTGAGCCTGGCCAG
  TGAAGTCCAGGACCTGCATCTGGCCCAGAGGAAGGAAATGGCCTCAGGCTTCAGCAAGGGGCCCACCCTG
  GGGCTGCTGCCCGACGTGCCCTCCCTGATGGAGACACTCAGCTACAGCTACTGCTACGTGGGAATCATGA
  CAGGCCCGTTCTTCCGCTACCGCACCTACCTGGACTGGCTGGAGCAGCCCTTCCCCGGGGCAGTGCCCAG
  CCTGCGGCCCCTGCTGCGCCGCGCCTGGCCGGCCCCGCTCTTCGGCCTGCTGTTCCTGCTCTCCTCTCAC
  CTCTTCCCGCTGGAGGCCGTGCGCGAGGACGCCTTCTACGCCCGCCCGCTGCCCGCCCGCCTCTTCTACA
  TGATCCCCGTCTTCTTCGCCTTCCGCATGCGCTTCTACGTGGCCTGGATTGCCGCCGAGTGCGGCTGCAT
  TGCCGCCGGCTTTGGGGCCTACCCCGTGGCCGCCAAAGCCCGGGCCGGAGGCGGCCCCACCCTCCAATGC
  CCACCCCCCAGCAGTCCGGAGAAGGCGGCTTCCTTGGAGTATGACTATGAGACCATCCGCAACATCGACT
  GCTACAGCACAGATTTCTGCGTGCGGGTGCGCGATGGCATGCGGTACTGGAACATGACGGTGCAGTGGTG
  GCTGGCGCAGTATATCTACAAGAGCGCACCTGCCCGTTCCTATGTCCTGCGGAGCGCCTGGACCATGCTG
  CTGAGCGCCTACTGGCACGGCCTCCACCCGGGCTACTACCTGAGCTTCCTGACCATCCCGCTGTGCCTGG
  CTGCCGAGGGCCGGCTGGAGTCAGCCCTGCGGGGGCGGCTGAGCCCAGGGGGCCAGAAGGCCTGGGACTG
  GGTGCACTGGTTCCTGAAGATGCGCGCCTATGACTACATGTGCATGGGCTTCGTGCTGCTCTCCTTGGCC
  GACACCCTTCGGTACTGGGCCTCCATCTACTTCTGTATCCACTTCCTGGCCCTGGCAGCCCTGGGGCTGG
  GGCTGGCTTTAGGTGGGGGCAGCCCCAGCCGGCGGAAGGCAGCATCCCAGCCCACCAGCCTTGCCCCGGA
  GAAGCTCCGGGAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201320 representing NM_024298
  Red=Cloning site Green=Tags(s)
  
  MSPEEWTYLVVLLISIPIGFLFKKAGPGLKRWGAAAVGLGLTLFTCGPHTLHSLVTILGTWALIQAQPCS
  CHALALAWTFSYLLFFRALSLLGLPTPTPFTNAVQLLLTLKLVSLASEVQDLHLAQRKEMASGFSKGPTL
  GLLPDVPSLMETLSYSYCYVGIMTGPFFRYRTYLDWLEQPFPGAVPSLRPLLRRAWPAPLFGLLFLLSSH
  LFPLEAVREDAFYARPLPARLFYMIPVFFAFRMRFYVAWIAAECGCIAAGFGAYPVAAKARAGGGPTLQC
  PPPSSPEKAASLEYDYETIRNIDCYSTDFCVRVRDGMRYWNMTVQWWLAQYIYKSAPARSYVLRSAWTML
  LSAYWHGLHPGYYLSFLTIPLCLAAEGRLESALRGRLSPGGQKAWDWVHWFLKMRAYDYMCMGFVLLSLA
  DTLRYWASIYFCIHFLALAALGLGLALGGGSPSRRKAASQPTSLAPEKLREE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024298
• ORF Size: 1416 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024298.5
• RefSeq Size: 2529 bp
• RefSeq ORF: 1419 bp
• Locus ID: 79143
• UniProt ID: Q96N66
• Cytogenetics: 19q13.42
• Domains: MBOAT
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the membrane-bound O-acyltransferases family of integral membrane proteins that have acyltransferase activity. The encoded protein is a lysophosphatidylinositol acyltransferase that has specificity for arachidonoyl-CoA as an acyl donor. This protein is involved in the reacylation of phospholipids as part of the phospholipid remodeling pathway known as the Land cycle. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2009]