POLD1 (NM_002691) Human Tagged ORF Clone

CAT#: RG201238

POLD1 (tGFP-tagged) - Human polymerase (DNA directed), delta 1, catalytic subunit 125kDa (POLD1)

Product Images

Circular map for RG201238

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: POLD1
• Synonyms: CDC2; CRCS10; MDPL; POLD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201238 representing NM_002691
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGCAAGCGGCGGCCAGGCCCAGGGCCCGGGGTGCCCCCAAAGCGGGCCCGTGGGGGCCTCTGGG
  ATGATGATGATGCACCTCGGCCATCCCAATTCGAGGAGGACCTGGCACTGATGGAGGAGATGGAGGCAGA
  ACACAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCAGTCCTGGAGGGGGTTGCAGACGGGCAGGTC
  CCACCATCAGCCATAGATCCTCGCTGGCTTCGGCCCACACCACCAGCGCTGGACCCCCAGACAGAGCCCC
  TCATCTTCCAACAGTTGGAGATTGACCATTATGTGGGCCCAGCACAGCCTGTGCCTGGGGGGCCCCCACC
  ATCCCACGGCTCCGTGCCTGTGCTCCGCGCCTTCGGGGTCACCGATGAGGGGTTCTCTGTCTGCTGCCAC
  ATCCACGGCTTCGCTCCCTACTTCTACACCCCAGCGCCCCCTGGTTTCGGGCCCGAGCACATGGGTGACC
  TGCAACGGGAGCTGAACTTGGCCATCAGCCGGGACAGTCGCGGGGGGAGGGAGCTGACTGGGCCGGCCGT
  GCTGGCTGTGGAACTGTGCTCCCGAGAGAGCATGTTTGGGTACCACGGGCACGGCCCCTCCCCGTTCCTG
  CGCATCACCGTGGCGCTGCCGCGCCTCGTGGCCCCGGCCCGCCGTCTCCTGGAACAGGGCATCCGTGTGG
  CAGGCCTGGGCACGCCCAGCTTCGCGCCCTACGAGGCCAACGTCGACTTTGAGATCCGGTTCATGGTGGA
  CACGGACATCGTCGGCTGCAACTGGCTGGAGCTCCCAGCTGGGAAATACGCCCTGAGGCTGAAGGAGAAG
  GCTACGCAGTGCCAGCTGGAGGCGGACGTGCTGTGGTCTGACGTGGTCAGTCACCCACCGGAAGGGCCAT
  GGCAGCGCATTGCGCCCTTGCGCGTGCTCAGCTTCGATATCGAGTGCGCCGGCCGCAAAGGCATCTTCCC
  TGAGCCTGAGCGGGACCCTGTCATCCAGATCTGCTCGCTGGGCCTGCGCTGGGGGGAGCCGGAGCCCTTC
  CTACGCCTGGCGCTCACCCTGCGGCCCTGTGCCCCCATCCTGGGTGCCAAGGTGCAGAGCTACGAGAAGG
  AGGAGGACCTGCTGCAGGCCTGGTCCACCTTCATCCGTATCATGGACCCCGACGTGATCACCGGTTACAA
  CATCCAGAACTTCGACCTTCCGTACCTCATCTCTCGGGCCCAGACCCTCAAGGTACAAACATTCCCTTTC
  CTGGGCCGTGTGGCCGGCCTTTGCTCCAACATCCGGGACTCTTCATTCCAGTCCAAGCAGACGGGCCGGC
  GGGACACCAAGGTTGTCAGCATGGTGGGCCGCGTGCAGATGGACATGCTGCAGGTGCTGCTGCGGGAGTA
  CAAGCTCCGCTCCTACACGCTCAATGCCGTGAGCTTCCACTTCCTGGGCGAGCAGAAGGAGGACGTGCAG
  CACAGCATCATCACCGACCTGCAGAATGGGAACGACCAGACCCGCCGCCGCCTGGCTGTGTACTGCCTGA
  AGGATGCCTACCTGCCACTGCGGCTGCTGGAGCGGCTCATGGTGCTGGTGAACGCCGTGGAGATGGCGAG
  GGTCACTGGCGTGCCCCTCAGCTACCTGCTCAGTCGTGGCCAGCAGGTCAAGGTCGTATCCCAGCTGTTG
  CGGCAGGCCATGCACGAGGGGCTGCTGATGCCCGTGGTGAAGTCAGAGGGCGGCGAGGACTACACGGGAG
  CCACTGTCATCGAGCCCCTCAAAGGGTACTACGACGTCCCCATCGCCACCCTGGACTTCTCCTCGCTGTA
  CCCGTCCATCATGATGGCCCACAACCTGTGTTACACCACGCTCCTTCGGCCCGGGACTGCACAGAAACTG
  GGCCTGACTGAGGATCAGTTCATCAGGACCCCCACCGGGGACGAGTTTGTGAAGACCTCAGTGCGGAAGG
  GGCTGCTGCCCCAGATCCTGGAGAACCTGCTCAGTGCCCGGAAGAGGGCCAAGGCCGAGCTGGCCAAGGA
  GACAGACCCCCTCCGGCGCCAGGTCCTGGATGGACGGCAGCTGGCGCTGAAGGTGAGCGCCAACTCCGTA
  TACGGCTTCACTGGCGCCCAGGTGGGCAAGTTGCCGTGCCTGGAGATCTCACAGAGCGTCACGGGGTTCG
  GACGTCAGATGATCGAGAAAACCAAGCAGCTGGTGGAGTCTAAGTACACAGTGGAGAATGGCTACAGCAC
  CAGTGCCAAGGTGGTGTATGGTGACACTGACTCCGTCATGTGCCGATTCGGCGTGTCCTCGGTGGCTGAG
  GCGATGGCCCTGGGGCGGGAGGCCGCGGACTGGGTGTCAGGTCACTTCCCGTCGCCCATCCGGCTGGAGT
  TTGAGAAGGTCTACTTCCCATACCTGCTTATCAGCAAGAAGCGCTACGCGGGCCTGCTCTTCTCCTCCCG
  GCCCGACGCCCACGACCGCATGGACTGCAAGGGCCTGGAGGCCGTGCGCAGGGACAACTGCCCCCTCGTG
  GCCAACCTGGTCACTGCCTCACTGCGCCGCCTGCTCATCGACCGAGACCCTGAGGGCGCGGTGGCTCACG
  CACAGGACGTCATCTCGGACCTGCTGTGCAACCGCATCGATATCTCCCAGCTGGTCATCACCAAGGAGCT
  GACCCGCGCGGCCTCCGACTATGCCGGCAAGCAGGCCCACGTGGAGCTGGCCGAGAGGATGAGGAAGCGG
  GACCCCGGGAGTGCGCCCAGCCTGGGCGACCGCGTCCCCTACGTGATCATCAGTGCCGCCAAGGGTGTGG
  CCGCCTACATGAAGTCGGAGGACCCGCTGTTCGTGCTGGAGCACAGCCTGCCCATTGACACGCAGTACTA
  CCTGGAGCAGCAGCTGGCCAAGCCCCTCCTGCGCATCTTCGAGCCCATCCTGGGCGAGGGCCGTGCCGAG
  GCTGTGCTACTGCGGGGGGACCACACGCGCTGCAAGACGGTGCTCACGGGCAAGGTGGGCGGCCTCCTGG
  CCTTCGCCAAACGCCGCAACTGCTGCATTGGCTGCCGCACAGTGCTCAGCCACCAGGGAGCCGTGTGTGA
  GTTCTGCCAGCCCCGGGAGTCTGAGCTGTATCAGAAGGAGGTATCCCATCTGAATGCCCTGGAGGAGCGC
  TTCTCGCGCCTCTGGACGCAGTGCCAGCGCTGCCAGGGCAGCCTGCACGAGGACGTCATCTGCACCAGCC
  GGGACTGCCCCATCTTCTACATGCGCAAGAAGGTGCGGAAGGACCTGGAAGACCAGGAGCAGCTCCTGCG
  GCGCTTCGGACCCCCTGGACCTGAGGCCTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201238 representing NM_002691
  Red=Cloning site Green=Tags(s)
  
  MDGKRRPGPGPGVPPKRARGGLWDDDDAPRPSQFEEDLALMEEMEAEHRLQEQEEEELQSVLEGVADGQV
  PPSAIDPRWLRPTPPALDPQTEPLIFQQLEIDHYVGPAQPVPGGPPPSHGSVPVLRAFGVTDEGFSVCCH
  IHGFAPYFYTPAPPGFGPEHMGDLQRELNLAISRDSRGGRELTGPAVLAVELCSRESMFGYHGHGPSPFL
  RITVALPRLVAPARRLLEQGIRVAGLGTPSFAPYEANVDFEIRFMVDTDIVGCNWLELPAGKYALRLKEK
  ATQCQLEADVLWSDVVSHPPEGPWQRIAPLRVLSFDIECAGRKGIFPEPERDPVIQICSLGLRWGEPEPF
  LRLALTLRPCAPILGAKVQSYEKEEDLLQAWSTFIRIMDPDVITGYNIQNFDLPYLISRAQTLKVQTFPF
  LGRVAGLCSNIRDSSFQSKQTGRRDTKVVSMVGRVQMDMLQVLLREYKLRSYTLNAVSFHFLGEQKEDVQ
  HSIITDLQNGNDQTRRRLAVYCLKDAYLPLRLLERLMVLVNAVEMARVTGVPLSYLLSRGQQVKVVSQLL
  RQAMHEGLLMPVVKSEGGEDYTGATVIEPLKGYYDVPIATLDFSSLYPSIMMAHNLCYTTLLRPGTAQKL
  GLTEDQFIRTPTGDEFVKTSVRKGLLPQILENLLSARKRAKAELAKETDPLRRQVLDGRQLALKVSANSV
  YGFTGAQVGKLPCLEISQSVTGFGRQMIEKTKQLVESKYTVENGYSTSAKVVYGDTDSVMCRFGVSSVAE
  AMALGREAADWVSGHFPSPIRLEFEKVYFPYLLISKKRYAGLLFSSRPDAHDRMDCKGLEAVRRDNCPLV
  ANLVTASLRRLLIDRDPEGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKR
  DPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAE
  AVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEER
  FSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002691
• ORF Size: 3321 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002691.1, NP_002682.1
• RefSeq Size: 3443 bp
• RefSeq ORF: 3324 bp
• Locus ID: 5424
• UniProt ID: P28340
• Cytogenetics: 19q13.33
• Domains: DNA_pol_B_exo, DNA_pol_B, POLBc
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]