PROX1 (NM_002763) Human Tagged ORF Clone

CAT#: RG201140

PROX1 (tGFP-tagged) - Human prospero homeobox 1 (PROX1)

Product Images

Circular map for RG201140

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PROX1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201140 representing NM_002763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGACCATGACAGCACAGCCCTCTTAAGCCGGCAAACCAAGAGGAGAAGAGTTGACATTGGAGTGA
  AAAGGACGGTAGGGACAGCATCTGCATTTTTTGCTAAGGCAAGAGCAACGTTTTTTAGTGCCATGAATCC
  CCAAGGTTCTGAGCAGGATGTTGAGTATTCAGTGGTGCAGCATGCAGATGGGGAAAAGTCAAATGTACTC
  CGCAAGCTGCTGAAGAGGGCGAACTCGTATGAAGATGCCATGATGCCTTTTCCAGGAGCAACCATAATTT
  CCCAGCTGTTGAAAAATAACATGAACAAAAATGGTGGCACGGAGCCCAGTTTCCAAGCCAGCGGTCTCTC
  TAGTACAGGCTCCGAAGTACATCAGGAGGATATATGCAGCAACTCTTCAAGAGACAGCCCCCCAGAGTGT
  CTTTCCCCTTTTGGCAGGCCTACTATGAGCCAGTTTGATATGGATCGCTTATGTGATGAGCACCTGAGAG
  CAAAGCGCGCCCGGGTTGAGAATATAATTCGGGGTATGAGCCATTCCCCCAGTGTGGCATTAAGGGGCAA
  TGAAAATGAAAGAGAGATGGCCCCGCAGTCTGTGAGTCCCCGAGAAAGTTACAGAGAAAACAAACGCAAG
  CAAAAGCTTCCCCAGCAGCAGCAACAGAGTTTCCAGCAGCTGGTTTCAGCCCGAAAAGAACAGAAGCGAG
  AGGAGCGCCGACAGCTGAAACAGCAGCTGGAGGACATGCAGAAACAGCTGCGCCAGCTGCAGGAAAAGTT
  CTACCAAATCTATGACAGCACTGATTCGGAAAATGATGAAGATGGTAACCTGTCTGAAGACAGCATGCGC
  TCGGAGATCCTGGATGCCAGGGCCCAGGACTCTGTCGGAAGGTCAGATAATGAGATGTGCGAGCTAGACC
  CAGGACAGTTTATTGACCGAGCTCGAGCCCTGATCAGAGAGCAGGAAATGGCTGAAAACAAGCCGAAGCG
  AGAAGGCAACAACAAAGAAAGAGACCATGGGCCAAACTCCTTACAACCGGAAGGCAAACATTTGGCTGAG
  ACCTTGAAACAGGAACTGAACACTGCCATGTCGCAAGTTGTGGACACTGTGGTCAAAGTCTTTTCGGCCA
  AGCCCTCCCGCCAGGTTCCTCAGGTCTTCCCACCTCTCCAGATCCCCCAGGCCAGATTTGCAGTCAATGG
  GGAAAACCACAATTTCCACACCGCCAACCAGCGCCTGCAGTGCTTTGGCGACGTCATCATTCCGAACCCC
  CTGGACACCTTTGGCAATGTGCAGATGGCCAGTTCCACTGACCAGACAGAAGCACTGCCCCTGGTTGTCC
  GCAAAAACTCCTCTGACCAGTCTGCCTCCGGCCCTGCCGCTGGCGGCCACCACCAGCCCCTGCACCAGTC
  GCCTCTCTCTGCCACCACGGGCTTCACCACGTCCACCTTCCGCCACCCCTTCCCCCTTCCCTTGATGGCC
  TATCCATTTCAGAGCCCATTAGGTGCTCCCTCCGGCTCCTTCTCTGGAAAAGACAGAGCCTCTCCTGAAT
  CCTTAGACTTAACTAGGGATACCACGAGTCTGAGGACCAAGATGTCATCTCACCACCTGAGCCACCACCC
  TTGTTCACCAGCACACCCGCCCAGCACCGCCGAAGGGCTCTCCTTGTCGCTCATAAAGTCCGAGTGCGGC
  GATCTTCAAGATATGTCTGAAATATCACCTTATTCGGGAAGTGCAATGCAGGAAGGATTGTCACCCAATC
  ACTTGAAAAAAGCAAAGCTCATGTTTTTTTATACCCGTTATCCCAGCTCCAATATGCTGAAGACCTACTT
  CTCCGACGTAAAGTTCAACAGATGCATTACCTCTCAGCTCATCAAGTGGTTTAGCAATTTCCGTGAGTTT
  TACTACATTCAGATGGAGAAGTACGCACGTCAAGCCATCAACGATGGGGTCACCAGTACTGAAGAGCTGT
  CTATAACCAGAGACTGTGAGCTGTACAGGGCTCTGAACATGCACTACAATAAAGCAAATGACTTTGAGGT
  TCCAGAGAGATTCCTGGAAGTTGCTCAGATCACATTACGGGAGTTTTTCAATGCCATTATCGCAGGCAAA
  GATGTTGATCCTTCCTGGAAGAAGGCCATATACAAGGTCATCTGCAAGCTGGATAGTGAAGTCCCTGAGA
  TTTTCAAATCCCCGAACTGCCTACAAGAGCTGCTTCATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201140 representing NM_002763
  Red=Cloning site Green=Tags(s)
  
  MPDHDSTALLSRQTKRRRVDIGVKRTVGTASAFFAKARATFFSAMNPQGSEQDVEYSVVQHADGEKSNVL
  RKLLKRANSYEDAMMPFPGATIISQLLKNNMNKNGGTEPSFQASGLSSTGSEVHQEDICSNSSRDSPPEC
  LSPFGRPTMSQFDMDRLCDEHLRAKRARVENIIRGMSHSPSVALRGNENEREMAPQSVSPRESYRENKRK
  QKLPQQQQQSFQQLVSARKEQKREERRQLKQQLEDMQKQLRQLQEKFYQIYDSTDSENDEDGNLSEDSMR
  SEILDARAQDSVGRSDNEMCELDPGQFIDRARALIREQEMAENKPKREGNNKERDHGPNSLQPEGKHLAE
  TLKQELNTAMSQVVDTVVKVFSAKPSRQVPQVFPPLQIPQARFAVNGENHNFHTANQRLQCFGDVIIPNP
  LDTFGNVQMASSTDQTEALPLVVRKNSSDQSASGPAAGGHHQPLHQSPLSATTGFTTSTFRHPFPLPLMA
  YPFQSPLGAPSGSFSGKDRASPESLDLTRDTTSLRTKMSSHHLSHHPCSPAHPPSTAEGLSLSLIKSECG
  DLQDMSEISPYSGSAMQEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREF
  YYIQMEKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIIAGK
  DVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002763
• ORF Size: 2211 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002763.5
• RefSeq Size: 3088 bp
• RefSeq ORF: 2214 bp
• Locus ID: 5629
• UniProt ID: Q92786
• Cytogenetics: 1q32.3
• Domains: Prox1
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS
• Gene Summary: The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]