Cytokeratin 7 (KRT7) (NM_005556) Human Tagged ORF Clone

CAT#: RG201124

KRT7 (tGFP-tagged) - Human keratin 7 (KRT7)

Product Images

Circular map for RG201124

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cytokeratin 7
• Synonyms: CK7; K2C7; K7; SCL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201124 representing NM_005556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCATCCACTTCAGCTCCCCGGTATTCACCTCGCGCTCAGCCGCCTTCTCGGGCCGCGGCGCCCAGG
  TGCGCCTGAGCTCCGCTCGCCCCGGCGGCCTTGGCAGCAGCAGCCTCTACGGCCTCGGCGCCTCGCGGCC
  GCGCGTGGCCGTGCGCTCTGCCTATGGGGGCCCGGTGGGCGCCGGCATCCGCGAGGTCACCATTAACCAG
  AGCCTGCTGGCCCCGCTGCGGCTGGACGCCGACCCCTCCCTCCAGCGGGTGCGCCAGGAGGAGAGCGAGC
  AGATCAAGACCCTCAACAACAAGTTTGCCTCCTTCATCGACAAGGTGCGGTTTCTGGAGCAGCAGAACAA
  GCTGCTGGAGACCAAGTGGACGCTGCTGCAGGAGCAGAAGTCGGCCAAGAGCAGCCGCCTCCCAGACATC
  TTTGAGGCCCAGATTGCTGGCCTTCGGGGTCAGCTTGAGGCACTGCAGGTGGATGGGGGCCGCCTGGAGG
  CGGAGCTGCGGAGCATGCAGGATGTGGTGGAGGACTTCAAGAATAAGTACGAAGATGAAATTAACCGCCG
  CACAGCTGCTGAGAATGAGTTTGTGGTGCTGAAGAAGGATGTGGATGCTGCCTACATGAGCAAGGTGGAG
  CTGGAGGCCAAGGTGGATGCCCTGAATGATGAGATCAACTTCCTCAGGACCCTCAATGAGACGGAGTTGA
  CAGAGCTGCAGTCCCAGATCTCCGACACATCTGTGGTGCTGTCCATGGACAACAGTCGCTCCCTGGACCT
  GGACGGCATCATCGCTGAGGTCAAGGCACAGTATGAGGAGATGGCCAAATGCAGCCGGGCTGAGGCTGAA
  GCCTGGTACCAGACCAAGTTTGAGACCCTCCAGGCCCAGGCTGGGAAGCATGGGGACGACCTCCGGAATA
  CCCGGAATGAGATTTCAGAGATGAACCGGGCCATCCAGAGGCTGCAGGCTGAGATCGACAACATCAAGAA
  CCAGCGTGCCAAGTTGGAGGCCGCCATTGCCGAGGCTGAGGAGCGTGGGGAGCTGGCGCTCAAGGATGCT
  CGTGCCAAGCAGGAGGAGCTGGAAGCCGCCCTGCAGCGGGCCAAGCAGGATATGGCACGGCAGCTGCGTG
  AGTACCAGGAACTCATGAGCGTGAAGCTGGCCCTGGACATCGAGATCGCCACCTACCGCAAGCTGCTGGA
  GGGCGAGGAGAGCCGGTTGGCTGGAGATGGAGTGGGAGCCGTGAATATCTCTGTGATGAATTCCACTGGT
  GGCAGTAGCAGTGGCGGTGGCATTGGGCTGACCCTCGGGGGAACCATGGGCAGCAATGCCCTGAGCTTCT
  CCAGCAGTGCGGGTCCTGGGCTCCTGAAGGCTTATTCCATCCGGACCGCATCCGCCAGTCGCAGGAGTGC
  CCGCGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201124 representing NM_005556
  Red=Cloning site Green=Tags(s)
  
  MSIHFSSPVFTSRSAAFSGRGAQVRLSSARPGGLGSSSLYGLGASRPRVAVRSAYGGPVGAGIREVTINQ
  SLLAPLRLDADPSLQRVRQEESEQIKTLNNKFASFIDKVRFLEQQNKLLETKWTLLQEQKSAKSSRLPDI
  FEAQIAGLRGQLEALQVDGGRLEAELRSMQDVVEDFKNKYEDEINRRTAAENEFVVLKKDVDAAYMSKVE
  LEAKVDALNDEINFLRTLNETELTELQSQISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAE
  AWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDA
  RAKQEELEAALQRAKQDMARQLREYQELMSVKLALDIEIATYRKLLEGEESRLAGDGVGAVNISVMNSTG
  GSSSGGGIGLTLGGTMGSNALSFSSSAGPGLLKAYSIRTASASRRSARD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005556
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005556.2
• RefSeq Size: 1753 bp
• RefSeq ORF: 1410 bp
• Locus ID: 3855
• UniProt ID: P08729
• Cytogenetics: 12q13.13
• Domains: filament
• Protein Families: ES Cell Differentiation/IPS
• Gene Summary: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]