COPZ1 (NM_016057) Human Tagged ORF Clone

CAT#: RG201023

COPZ1 (tGFP-tagged) - Human coatomer protein complex, subunit zeta 1 (COPZ1)

Product Images

Circular map for RG201023

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: COPZ1
• Synonyms: CGI-120; COPZ; HSPC181; zeta-COP; zeta1-COP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201023 representing NM_016057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTGATTTTGGAACCTTCCCTGTATACTGTCAAAGCCATCCTGATTCTGGACAATGATGGAG
  ATCGACTTTTTGCCAAGTACTATGACGACACCTACCCCAGTGTCAAGGAGCAAAAGGCCTTTGAGAAGAA
  CATTTTCAACAAGACCCATCGGACTGACAGTGAAATTGCCCTCTTGGAAGGCCTGACAGTGGTATACAAA
  AGCAGTATAGATCTCTATTTCTATGTGATTGGCAGCTCCTATGAAAATGAGCTGATGCTTATGGCTGTTC
  TGAACTGTCTCTTCGACTCATTGAGCCAGATGCTGAGGAAAAATGTAGAAAAGCGAGCACTGCTGGAGAA
  CATGGAGGGGCTGTTCTTGGCTGTGGATGAAATTGTAGATGGAGGGGTGATCCTAGAGAGTGATCCCCAG
  CAGGTGGTACACCGGGTGGCATTAAGGGGTGAAGATGTCCCCCTTACGGAGCAGACCGTGTCTCAGGTGC
  TGCAGTCAGCCAAAGAACAGATCAAGTGGTCACTCCTTCGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201023 representing NM_016057
  Red=Cloning site Green=Tags(s)
  
  MEALILEPSLYTVKAILILDNDGDRLFAKYYDDTYPSVKEQKAFEKNIFNKTHRTDSEIALLEGLTVVYK
  SSIDLYFYVIGSSYENELMLMAVLNCLFDSLSQMLRKNVEKRALLENMEGLFLAVDEIVDGGVILESDPQ
  QVVHRVALRGEDVPLTEQTVSQVLQSAKEQIKWSLLR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016057
• ORF Size: 531 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016057.3
• RefSeq Size: 1900 bp
• RefSeq ORF: 534 bp
• Locus ID: 22818
• UniProt ID: P61923
• Cytogenetics: 12q13.13
• Domains: Clat_adaptor_s
• Gene Summary: This gene encodes a subunit of the cytoplasmic coatamer protein complex, which is involved in autophagy and intracellular protein trafficking. The coatomer protein complex is comprised of seven subunits and functions as the coat protein of coat protein complex (COP)I-vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]