C8orf30A (HGH1) (NM_016458) Human Tagged ORF Clone

CAT#: RG200877

FAM203A (tGFP-tagged) - Human family with sequence similarity 203, member A (FAM203A)

Product Images

Circular map for RG200877

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C8orf30A
• Synonyms: BRP16; BRP16L; C8orf30A; C8orf30B; FAM203A; FAM203B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200877 representing NM_016458
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAGGCCGGGGCTGGCGCTGGCGCCTCGGGAGGGCCGGAGGCAAGCCCGGAGGCAGAGGTGGTGA
  AGCTGCTGCCCTTCCTGGCGCCGGGCGCGCGGGCGGACCTGCAGGCGGCGGCGGTGCGGCACGTGCTGGC
  GCTGACTGGCTGCGGACCCGGCCGCGCGCTGTTGGCGGGGCAGGCGGCGCTGCTGCAGGCGCTGATGGAG
  CTGGCGCCGGCCTCTGCCCCGGCCCGGGACGCCGCCCGCGCGCTCGTGAACTTGGCCGCCGACCCCGGCC
  TGCACGAGACATTGCTGGCGGCCGACCCCGGGCTGCCAGCGCGCCTGATGGGCCGCGCGTTGGACCCGCA
  GTGGCCCTGGGCCGAGGAGGCGGCCGCCGCGCTAGCCAACCTCAGTCGCGAGCCGGCGCCGTGTGCAGCG
  CTCATGGCGGCGCTGGCGGCCGCGGAGCCGGCAGACTCGGGCCTGGAGCGGCTGGTGCGCGCGCTGTGCA
  CGCCCGGCTACAACGCCCGCGCGCCCCTGCACTACCTAGCGCCGCTGCTCTCCAACCTCAGCCAACGCCC
  TGCGGCGCGGGCCTTCCTACTGGACCCCGACAGGTGCGTGGTCCAGCGGCTGCTGCCCCTTACCCAGTAC
  CCCGACTCCTCTGTACGCAGGGGCGGGGTGGTGGGGACGCTGCGGAATTGCTGCTTCGAGCACCGACATC
  ACGAGTGGTTGCTTGGACCTGAGGTGGACATTCTCCCCTTTTTGCTCCTGCCCTTGGCTGGGCCTGAGGA
  TTTCTCCGAGGAAGAGATGGAACGGCTGCCTGTCGACTTGCAGTACCTGCCACCAGACAAGCAGCGAGAA
  CCTGATGCAGACATCCGCAAGATGCTTGTTGAAGCCATCATGCTGCTGACAGCCACAGCACCAGGTCGGC
  AGCAGGTGCGGGACCAGGGAGCCTACCTGATCCTTCGAGAGCTGCACAGCTGGGAGCCGGAGCCCGACGT
  GCGGACGGCTTGTGAGAAGCTCATCCAGGTGCTTATTGGGGACGAGCCTGAACGTGGCATGGAAAACCTG
  CTGGAGGTGCAGGTGCCTGAGGATGTGGAGCAGCAGCTGCAGCAGCTGGATTGCAGGGAGCAGGAGCAGT
  TGGAGCGGGAGCTGGCCCCAGAGCCATGGGTGGAGAGGGCCACACCCACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200877 representing NM_016458
  Red=Cloning site Green=Tags(s)
  
  MGEAGAGAGASGGPEASPEAEVVKLLPFLAPGARADLQAAAVRHVLALTGCGPGRALLAGQAALLQALME
  LAPASAPARDAARALVNLAADPGLHETLLAADPGLPARLMGRALDPQWPWAEEAAAALANLSREPAPCAA
  LMAALAAAEPADSGLERLVRALCTPGYNARAPLHYLAPLLSNLSQRPAARAFLLDPDRCVVQRLLPLTQY
  PDSSVRRGGVVGTLRNCCFEHRHHEWLLGPEVDILPFLLLPLAGPEDFSEEEMERLPVDLQYLPPDKQRE
  PDADIRKMLVEAIMLLTATAPGRQQVRDQGAYLILRELHSWEPEPDVRTACEKLIQVLIGDEPERGMENL
  LEVQVPEDVEQQLQQLDCREQEQLERELAPEPWVERATPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016458
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016458.4
• RefSeq Size: 2441 bp
• RefSeq ORF: 1173 bp
• Locus ID: 51236
• UniProt ID: Q9BTY7
• Cytogenetics: 8q24.3
• Domains: DUF383, DUF384