POLR2F (NM_021974) Human Tagged ORF Clone
CAT#: RG200855
- TrueORF®
POLR2F (tGFP-tagged) - Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F)
ORF Plasmid: DDK
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_021974" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | POLR2F |
Synonyms | HRBP14.4; POLRF; RPABC2; RPABC14.4; RPB6; RPB14.4; RPC15 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG200855 representing NM_021974
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGACAACGAGGACAATTTTGATGGCGACGACTTTGATGATGTGGAGGAGGATGAAGGGCTAGATG ACTTGGAGAATGCCGAAGAGGAAGGCCAGGAGAATGTCGAGATCCTCCCCTCTGGGGAGCGACCGCAGGC CAACCAGAAGCGAATCACCACACCATACATGACCAAGTACGAGCGAGCCCGCGTGCTGGGCACCCGAGCG CTCCAGATTGCGATGTGTGCCCCTGTGATGGTGGAGCTGGAGGGGGAGACAGATCCTCTGCTCATTGCCA TGAAGGAACTCAAGGCCCGAAAGATCCCCATCATCATTCGCCGTTACCTGCCAGATGGGAGCTATGAAGA CTGGGGGGTGGACGAGCTCATCATCACCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG200855 representing NM_021974
Red=Cloning site Green=Tags(s) MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRA LQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_021974 |
ORF Size | 381 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021974.5 |
RefSeq Size | 546 bp |
RefSeq ORF | 384 bp |
Locus ID | 5435 |
UniProt ID | P61218 |
Cytogenetics | 22q13.1 |
Domains | RNA_pol_Rpb6 |
Protein Families | Transcription Factors |
Protein Pathways | Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
Gene Summary | This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200855 | POLR2F (Myc-DDK-tagged)-Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F) |
USD 150.00 |
|
RC200855L1 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), Myc-DDK-tagged |
USD 450.00 |
|
RC200855L2 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mGFP tagged |
USD 450.00 |
|
RC200855L3 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), Myc-DDK-tagged |
USD 450.00 |
|
RC200855L4 | Lenti ORF clone of Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F), mGFP tagged |
USD 450.00 |
|
SC319478 | POLR2F (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide F (POLR2F) |
USD 150.00 |
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