PECR (NM_018441) Human Tagged ORF Clone

CAT#: RG200822

PECR (tGFP-tagged) - Human peroxisomal trans-2-enoyl-CoA reductase (PECR)

Product Images

Circular map for RG200822

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PECR
• Synonyms: DCRRP; HPDHASE; HSA250303; PVIARL; SDR29C1; TERP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200822 representing NM_018441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTGGGCTAAGGGCAGGAGCTACCTGGCGCCTGGTTTGCTGCAGGGCCAAGTGGCCATCGTCA
  CCGGCGGGGCCACGGGCATCGGAAAAGCCATCGTGAAGGAGCTCCTGGAGCTGGGGAGTAATGTGGTCAT
  TGCATCCCGTAAGTTGGAGAGATTGAAGTCTGCGGCAGATGAACTGCAGGCCAACCTACCTCCCACAAAG
  CAGGCACGAGTCATTCCCATACAATGCAACATCCGGAATGAGGAGGAGGTGAATAATTTGGTCAAATCTA
  CCTTAGATACTTTTGGTAAGATCAATTTCTTGGTGAACAATGGAGGAGGCCAGTTTCTTTCCCCTGCTGA
  ACACATCAGTTCTAAGGGATGGCACGCTGTGCTTGAGACCAACCTGACGGGTACCTTCTACATGTGCAAA
  GCAGTTTACAGCTCCTGGATGAAAGAGCATGGAGGATCTATCGTCAATATCATTGTCCCTACTAAAGCTG
  GATTTCCATTAGCTGTGCATTCTGGAGCTGCAAGAGCAGGTGTTTACAACCTCACCAAATCTTTAGCTTT
  GGAATGGGCCTGCAGTGGAATACGGATCAATTGTGTTGCCCCTGGAGTTATTTATTCCCAGACTGCTGTG
  GAGAACTATGGTTCCTGGGGACAAAGCTTCTTTGAAGGGTCTTTTCAGAAAATCCCCGCTAAACGAATTG
  GTGTTCCTGAGGAGGTCTCCTCTGTGGTCTGCTTCCTACTGTCTCCTGCAGCTTCCTTCATCACTGGACA
  GTCAGTGGATGTGGATGGGGGCCGGAGTCTCTATACTCACTCGTATGAGGTACCAGATCATGACAACTGG
  CCCAAGGGAGCAGGGGACCTTTCTGTTGTCAAAAAGATGAAGGAGACCTTTAAGGAGAAAGCTAAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200822 representing NM_018441
  Red=Cloning site Green=Tags(s)
  
  MASWAKGRSYLAPGLLQGQVAIVTGGATGIGKAIVKELLELGSNVVIASRKLERLKSAADELQANLPPTK
  QARVIPIQCNIRNEEEVNNLVKSTLDTFGKINFLVNNGGGQFLSPAEHISSKGWHAVLETNLTGTFYMCK
  AVYSSWMKEHGGSIVNIIVPTKAGFPLAVHSGAARAGVYNLTKSLALEWACSGIRINCVAPGVIYSQTAV
  ENYGSWGQSFFEGSFQKIPAKRIGVPEEVSSVVCFLLSPAASFITGQSVDVDGGRSLYTHSYEVPDHDNW
  PKGAGDLSVVKKMKETFKEKAKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018441
• ORF Size: 909 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018441.2, NP_060911.2
• RefSeq Size: 1863 bp
• RefSeq ORF: 912 bp
• Locus ID: 55825
• UniProt ID: Q9BY49
• Cytogenetics: 2q35
• Domains: adh_short
• Protein Families: Druggable Genome
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• Gene Summary: Participates in chain elongation of fatty acids. Has no 2,4-dienoyl-CoA reductase activity.[UniProtKB/Swiss-Prot Function]