MAN2B1 (NM_000528) Human Tagged ORF Clone

CAT#: RG200638

MAN2B1 (tGFP-tagged) - Human mannosidase, alpha, class 2B, member 1 (MAN2B1), transcript variant 1

Product Images

Circular map for RG200638

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAN2B1
• Synonyms: LAMAN; MANB
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200638 representing NM_000528
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCTACGCGCGGGCTTCGGGGGTCTGCGCTCGCGGCTGCCTGGACTCAGCAGGCCCCTGGACCA
  TGTCCCGCGCCCTGCGGCCACCGCTCCCGCCTCTCTGCTTTTTCCTTTTGTTGCTGGCGGCTGCCGGTGC
  TCGGGCCGGGGGATACGAGACATGCCCCACAGTGCAGCCGAACATGCTGAACGTGCACCTGCTGCCTCAC
  ACACATGATGACGTGGGCTGGCTCAAAACCGTGGACCAGTACTTTTATGGAATCAAGAATGACATCCAGC
  ACGCCGGTGTGCAGTACATCCTGGACTCGGTCATCTCTGCCTTGCTGGCAGATCCCACCCGTCGCTTCAT
  TTACGTGGAGATTGCCTTCTTCTCCCGTTGGTGGCACCAGCAGACAAATGCCACACAGGAAGTCGTGCGA
  GACCTTGTGCGCCAGGGGCGCCTGGAGTTCGCCAATGGTGGCTGGGTGATGAACGATGAGGCAGCCACCC
  ACTACGGTGCCATCGTGGACCAGATGACACTTGGGCTGCGCTTTCTGGAGGACACATTTGGCAATGATGG
  GCGACCCCGTGTGGCCTGGCACATTGACCCCTTCGGCCACTCTCGGGAGCAGGCCTCGCTGTTTGCGCAG
  ATGGGCTTCGACGGCTTCTTCTTTGGGCGCCTTGATTATCAAGATAAGTGGGTACGGATGCAGAAGCTGG
  AGATGGAGCAGGTGTGGCGGGCCAGCACCAGCCTGAAGCCCCCGACCGCGGACCTCTTCACTGGTGTGCT
  TCCCAATGGTTACAACCCGCCAAGGAATCTGTGCTGGGATGTGCTGTGTGTCGATCAGCCGCTGGTGGAG
  GACCCTCGCAGCCCCGAGTACAACGCCAAGGAGCTGGTCGATTACTTCCTAAATGTGGCCACTGCCCAGG
  GCCGGTATTACCGCACCAACCACACTGTGATGACCATGGGCTCGGACTTCCAATATGAGAATGCCAACAT
  GTGGTTCAAGAACCTTGACAAGCTCATCCGGCTGGTAAATGCGCAGCAGGCAAAAGGAAGCAGTGTCCAT
  GTTCTCTACTCCACCCCCGCTTGTTACCTCTGGGAGCTGAACAAGGCCAACCTCACCTGGTCAGTGAAAC
  ATGACGACTTCTTCCCTTACGCGGATGGCCCCCACCAGTTCTGGACCGGTTACTTTTCCAGTCGGCCGGC
  CCTCAAACGCTACGAGCGCCTCAGCTACAACTTCCTGCAGGTGTGCAACCAGCTGGAGGCGCTGGTGGGC
  CTGGCGGCCAACGTGGGACCCTATGGCTCCGGAGACAGTGCACCCCTCAATGAGGCGATGGCTGTGCTCC
  AGCATCACGACGCCGTCAGCGGCACCTCCCGCCAGCACGTGGCCAACGACTACGCGCGCCAGCTTGCGGC
  AGGCTGGGGGCCTTGCGAGGTTCTTCTGAGCAACGCGCTGGCGCGGCTCAGAGGCTTCAAAGATCACTTC
  ACCTTTTGCCAACAGCTAAACATCAGCATCTGCCCGCTCAGCCAGACGGCGGCGCGCTTCCAGGTCATCG
  TTTATAATCCCCTGGGGCGGAAGGTGAATTGGATGGTACGGCTGCCGGTCAGCGAAGGCGTTTTCGTTGT
  GAAGGACCCCAATGGCAGGACAGTGCCCAGCGATGTGGTAATATTTCCCAGCTCAGACAGCCAGGCGCAC
  CCTCCGGAGCTGCTGTTCTCAGCCTCACTGCCCGCCCTGGGCTTCAGCACCTATTCAGTAGCCCAGGTGC
  CTCGCTGGAAGCCCCAGGCCCGCGCACCACAGCCCATCCCCAGAAGATCCTGGTCCCCTGCTTTAACCAT
  CGAAAATGAGCACATCCGGGCAACGTTTGATCCTGACACAGGGCTGTTGATGGAGATTATGAACATGAAT
  CAGCAACTCCTGCTGCCTGTTCGCCAGACCTTCTTCTGGTACAACGCCAGTATAGGTGACAACGAAAGTG
  ACCAGGCCTCAGGTGCCTACATCTTCAGACCCAACCAACAGAAACCGCTGCCTGTGAGCCGCTGGGCTCA
  GATCCACCTGGTGAAGACACCCTTGGTGCAGGAGGTGCACCAGAACTTCTCAGCTTGGTGTTCCCAGGTG
  GTTCGCCTGTACCCAGGACAGCGGCACCTGGAGCTAGAGTGGTCGGTGGGGCCGATACCTGTGGGCGACA
  CCTGGGGGAAGGAGGTCATCAGCCGTTTTGACACACCGCTGGAGACAAAGGGACGCTTCTACACAGACAG
  CAATGGCCGGGAGATCCTGGAGAGGAGGCGGGATTATCGACCCACCTGGAAACTGAACCAGACGGAGCCC
  GTGGCAGGAAACTACTATCCAGTCAACACCCGGATTTACATCACGGATGGAAACATGCAGCTGACTGTGC
  TGACTGACCGCTCCCAGGGGGGCAGCAGCCTGAGAGATGGCTCGCTGGAGCTCATGGTGCACCGAAGGCT
  GCTGAAGGACGATGGACGCGGAGTATCGGAGCCACTAATGGAGAACGGGTCGGGGGCGTGGGTGCGAGGG
  CGCCACCTGGTGCTGCTGGACACAGCCCAGGCTGCAGCCGCCGGACACCGGCTCCTGGCGGAGCAGGAGG
  TCCTGGCCCCTCAGGTGGTGCTGGCCCCGGGTGGCGGCGCCGCCTACAATCTCGGGGCTCCTCCGCGCAC
  GCAGTTCTCAGGGCTGCGCAGGGACCTGCCGCCCTCGGTGCACCTGCTCACGCTGGCCAGCTGGGGCCCC
  GAAATGGTGCTGCTGCGCTTGGAGCACCAGTTTGCCGTAGGAGAGGATTCCGGACGTAACCTGAGCGCCC
  CCGTTACCTTGAACTTGAGGGACCTGTTCTCCACCTTCACCATCACCCGCCTGCAGGAGACCACGCTGGT
  GGCCAACCAGCTCCGCGAGGCAGCCTCCAGGCTCAAGTGGACAACAAACACAGGCCCCACACCCCACCAA
  ACTCCGTACCAGCTGGACCCGGCCAACATCACGCTGGAACCCATGGAAATCCGCACTTTCCTGGCCTCAG
  TTCAATGGAAGGAGGTGGATGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200638 representing NM_000528
  Red=Cloning site Green=Tags(s)
  
  MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPH
  THDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVR
  DLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQ
  MGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVE
  DPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVH
  VLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVG
  LAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHF
  TFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAH
  PPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMN
  QQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQV
  VRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEP
  VAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRG
  RHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGP
  EMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQ
  TPYQLDPANITLEPMEIRTFLASVQWKEVDG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000528
• ORF Size: 3033 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000528.4
• RefSeq Size: 3468 bp
• RefSeq ORF: 3036 bp
• Locus ID: 4125
• UniProt ID: O00754
• Cytogenetics: 19p13.13
• Domains: Glyco_hydro_38
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Other glycan degradation
• Gene Summary: This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]