P2Y6 (P2RY6) (NM_176797) Human Tagged ORF Clone
P2RY6 (GFP-tagged) - Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 1
|Product Name||P2Y6 (P2RY6) (NM_176797) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Ampicillin (100 ug/mL)|
>RG200617 representing NM_176797
Red=Cloning site Blue=ORF Green=Tags(s)
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG200617 representing NM_176797
Red=Cloning site Green=Tags(s)
TRTRPLE - GFP Tag - V
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||984 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|Protein Families||Druggable Genome, GPCR, Transmembrane|
|Protein Pathways||Neuroactive ligand-receptor interaction|
|Gene Summary||The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013]|
|cDNA Clone Resources|
|SC109534||P2RY6 (untagged)-Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 1||
|SC320606||P2RY6 (untagged)-Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 1||
|RC200617||P2RY6 (Myc-DDK-tagged)-Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 1||
|RC200617L3||Lenti ORF clone of Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 1, Myc-DDK-tagged||
|RC200617L4||Lenti ORF clone of Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 1, mGFP tagged||