SSRP1 (NM_003146) Human Tagged ORF Clone

CAT#: RG200414

SSRP1 (tGFP-tagged) - Human structure specific recognition protein 1 (SSRP1)

Product Images

Circular map for RG200414

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SSRP1
• Synonyms: FACT; FACT80; T160
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200414 representing NM_003146
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGACACTGGAGTTCAACGACGTCTATCAGGAGGTGAAAGGTTCCATGAATGATGGTCGACTGA
  GGTTGAGCCGTCAGGGCATCATCTTCAAGAATAGCAAGACAGGCAAAGTGGACAACATCCAGGCTGGGGA
  GTTAACAGAAGGTATCTGGCGCCGTGTTGCTCTGGGCCATGGACTTAAACTGCTTACAAAGAATGGCCAT
  GTCTACAAGTATGATGGCTTCCGAGAATCGGAGTTTGAGAAACTCTCTGATTTCTTCAAAACTCACTATC
  GCCTTGAGCTAATGGAGAAGGACCTTTGTGTGAAGGGCTGGAACTGGGGGACAGTGAAATTTGGTGGGCA
  GCTGCTTTCCTTTGACATTGGTGACCAGCCAGTCTTTGAGATACCCCTCAGCAATGTGTCCCAGTGCACC
  ACAGGCAAGAATGAGGTGACACTGGAATTCCACCAAAACGATGACGCAGAGGTGTCTCTCATGGAGGTGC
  GCTTCTACGTCCCACCCACCCAGGAGGATGGTGTGGACCCTGTTGAGGCCTTTGCCCAGAATGTGTTGTC
  AAAGGCGGATGTAATCCAGGCCACGGGAGATGCCATCTGCATCTTCCGGGAGCTGCAGTGTCTGACTCCT
  CGTGGTCGTTATGACATTCGGATCTACCCCACCTTTCTGCACCTGCATGGCAAGACCTTTGACTACAAGA
  TCCCCTACACCACAGTACTGCGTCTGTTTTTGTTACCCCACAAGGACCAGCGCCAGATGTTCTTTGTGAT
  CAGCCTGGATCCCCCAATCAAGCAAGGCCAAACTCGCTACCACTTCCTGATCCTCCTCTTCTCCAAGGAC
  GAGGACATTTCGTTGACTCTGAACATGAACGAGGAAGAAGTGGAGAAGCGCTTTGAGGGTCGGCTCACCA
  AGAACATGTCAGGATCCCTCTATGAGATGGTCAGCCGGGTCATGAAAGCACTGGTAAACCGCAAGATCAC
  AGTGCCAGGCAACTTCCAAGGGCACTCAGGGGCCCAGTGCATTACCTGTTCCTACAAGGCAAGCTCAGGA
  CTGCTCTACCCGCTGGAGCGGGGCTTCATCTACGTCCACAAGCCACCTGTGCACATCCGCTTCGATGAGA
  TCTCCTTTGTCAACTTTGCTCGTGGTACCACTACTACTCGTTCCTTTGACTTTGAAATTGAGACCAAGCA
  GGGCACTCAGTATACCTTCAGCAGCATTGAGAGGGAGGAGTACGGGAAACTGTTTGATTTTGTCAACGCG
  AAAAAGCTCAACATCAAAAACCGAGGATTGAAAGAGGGCATGAACCCAAGCTACGATGAATATGCTGACT
  CTGATGAGGACCAGCATGATGCCTACTTGGAGAGGATGAAGGAGGAAGGCAAGATCCGGGAGGAGAATGC
  CAATGACAGCAGCGATGACTCAGGAGAAGAAACCGATGAGTCATTCAACCCAGGTGAAGAGGAGGAAGAT
  GTGGCAGAGGAGTTTGACAGCAACGCCTCTGCCAGCTCCTCCAGTAATGAGGGTGACAGTGACCGGGATG
  AGAAGAAGCGGAAACAGCTCAAAAAGGCCAAGATGGCCAAGGACCGCAAGAGCCGCAAGAAGCCTGTGGA
  GGTGAAGAAGGGCAAAGACCCCAATGCCCCCAAGAGGCCCATGTCTGCATACATGCTGTGGCTCAATGCC
  AGCCGAGAGAAGATCAAGTCAGACCATCCTGGCATCAGCATCACGGATCTTTCCAAGAAGGCAGGCGAGA
  TCTGGAAGGGAATGTCCAAAGAGAAGAAAGAGGAGTGGGATCGCAAGGCTGAGGATGCCAGGAGGGACTA
  TGAAAAAGCCATGAAAGAATATGAAGGGGGCCGAGGCGAGTCTTCTAAGAGGGACAAGTCAAAGAAGAAG
  AAGAAAGTAAAGGTAAAGATGGAAAAGAAATCCACGCCCTCTAGGGGCTCATCATCCAAGTCGTCCTCAA
  GGCAGCTAAGCGAGAGCTTCAAGAGCAAAGAGTTTGTGTCTAGTGATGAGAGCTCTTCGGGAGAGAACAA
  GAGCAAAAAGAAGAGGAGGAGGAGCGAGGACTCTGAAGAAGAAGAACTAGCCAGTACTCCCCCCAGCTCA
  GAGGACTCAGCGTCAGGATCCGATGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200414 representing NM_003146
  Red=Cloning site Green=Tags(s)
  
  MAETLEFNDVYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGH
  VYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCT
  TGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTP
  RGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKD
  EDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSG
  LLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNA
  KKLNIKNRGLKEGMNPSYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEED
  VAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPVEVKKGKDPNAPKRPMSAYMLWLNA
  SREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARRDYEKAMKEYEGGRGESSKRDKSKKK
  KKVKVKMEKKSTPSRGSSSKSSSRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSS
  EDSASGSDE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003146
• ORF Size: 2127 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003146.3
• RefSeq Size: 2825 bp
• RefSeq ORF: 2130 bp
• Locus ID: 6749
• UniProt ID: Q08945
• Cytogenetics: 11q12.1
• Domains: HMG, SSrecog
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is a subunit of a heterodimer that, along with SUPT16H, forms chromatin transcriptional elongation factor FACT. FACT interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT and cisplatin-damaged DNA may be crucial to the anticancer mechanism of cisplatin. This encoded protein contains a high mobility group box which most likely constitutes the structure recognition element for cisplatin-modified DNA. This protein also functions as a co-activator of the transcriptional activator p63. An alternatively spliced transcript variant of this gene has been described, but its full-length nature is not known. [provided by RefSeq, Jul 2008]