PSME3 (NM_005789) Human Tagged ORF Clone

CAT#: RG200308

PSME3 (tGFP-tagged) - Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki) (PSME3), transcript variant 1

Product Images

Circular map for RG200308

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PSME3
• Synonyms: HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; REG-GAMMA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200308 representing NM_005789
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTTGATTCTTTCAGGGAGCGGATCACAA
  GTGAGGCAGAAGACTTGGTGGCAAATTTTTTCCCAAAGAAGTTATTAGAACTTGATAGTTTTCTGAAGGA
  ACCAATCTTAAACATCCATGACCTAACTCAGATCCACTCTGACATGAATCTCCCAGTCCCTGACCCCATT
  CTTCTCACCAATAGCCATGATGGACTGGATGGTCCCACTTATAAGAAGCGAAGGTTGGATGAGTGTGAAG
  AAGCCTTCCAAGGAACCAAGGTGTTTGTGATGCCCAATGGGATGCTGAAAAGCAACCAGCAGCTGGTGGA
  CATTATTGAGAAAGTGAAACCTGAGATCCGGCTGTTGATTGAGAAATGTAACACGGTCAAAATGTGGGTA
  CAGCTCCTGATTCCCAGGATAGAAGATGGAAACAACTTTGGGGTGTCCATTCAGGAGGAAACAGTTGCAG
  AGCTAAGAACTGTTGAGAGTGAAGCTGCATCTTATCTGGACCAGATTTCTAGATATTATATTACAAGAGC
  CAAATTGGTTTCTAAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACCGTGACAGAGATTGAT
  GAGAAAGAATATATCAGCCTTCGGCTCATCATATCAGAGCTGAGGAATCAATATGTCACTCTACATGACA
  TGATCCTGAAAAATATCGAGAAGATCAAACGGCCCCGGAGCAGCAATGCAGAGACTCTGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200308 representing NM_005789
  Red=Cloning site Green=Tags(s)
  
  MASLLKVDQEVKLKVDSFRERITSEAEDLVANFFPKKLLELDSFLKEPILNIHDLTQIHSDMNLPVPDPI
  LLTNSHDGLDGPTYKKRRLDECEEAFQGTKVFVMPNGMLKSNQQLVDIIEKVKPEIRLLIEKCNTVKMWV
  QLLIPRIEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITRAKLVSKIAKYPHVEDYRRTVTEID
  EKEYISLRLIISELRNQYVTLHDMILKNIEKIKRPRSSNAETLY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005789
• ORF Size: 762 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005789.4
• RefSeq Size: 3189 bp
• RefSeq ORF: 765 bp
• Locus ID: 10197
• UniProt ID: P61289
• Cytogenetics: 17q21.31
• Domains: PA28_alpha, PA28_beta
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]