GPIP137 (CAPRIN1) (NM_005898) Human Tagged ORF Clone

CAT#: RG200300

CAPRIN1 (tGFP-tagged) - Human cell cycle associated protein 1 (CAPRIN1), transcript variant 1

Product Images

Circular map for RG200300

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPIP137
• Synonyms: GPIAP1; GPIP137; GRIP137; M11S1; p137GPI; RNG105
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200300 representing NM_005898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCGGCCACCAGCCACAGCGGGAGCGGCAGCAAGTCGTCCGGACCGCCACCGCCGTCGGGTTCCT
  CCGGGAGTGAGGCGGCCGCGGGAGCCGGGGCCGCCGCGCCGGCTTCTCAGCACCCCGCAACCGGCACCGG
  CGCTGTCCAGACCGAGGCCATGAAGCAGATTCTCGGGGTGATCGACAAGAAACTTCGGAACCTGGAGAAG
  AAAAAGGGTAAGCTTGATGATTACCAGGAACGAATGAACAAAGGGGAAAGGCTTAATCAAGATCAGCTGG
  ATGCCGTTTCTAAGTACCAGGAAGTCACAAATAATTTGGAGTTTGCAAAAGAATTACAGAGGAGTTTCAT
  GGCACTAAGTCAAGATATTCAGAAAACAATAAAGAAGACAGCACGTCGGGAGCAGCTTATGAGAGAAGAA
  GCTGAACAGAAACGTTTAAAAACTGTACTTGAGCTACAGTATGTTTTGGACAAATTGGGAGATGATGAAG
  TGCGGACTGACCTGAAACAAGGTTTGAATGGAGTGCCAATATTGTCCGAAGAGGAGTTGTCATTGTTGGA
  TGAATTCTATAAGCTAGTAGACCCTGAACGGGACATGAGCTTGAGGTTGAATGAACAGTATGAACATGCC
  TCCATTCACCTGTGGGACCTGCTGGAAGGGAAGGAAAAACCTGTATGTGGAACCACCTATAAAGTTCTAA
  AGGAAATTGTTGAGCGTGTTTTTCAGTCAAACTACTTTGACAGCACCCACAACCACCAGAATGGGCTGTG
  TGAGGAAGAAGAGGCAGCCTCAGCACCTGCAGTTGAAGACCAGGTACCTGAAGCTGAACCTGAGCCAGCA
  GAAGAGTACACTGAGCAAAGTGAAGTTGAATCAACAGAGTATGTAAATAGACAGTTCATGGCAGAAACAC
  AGTTCACCAGTGGTGAAAAGGAGCAGGTAGATGAGTGGACAGTTGAAACGGTTGAGGTGGTAAATTCACT
  CCAGCAGCAACCTCAGGCTGCATCCCCTTCAGTACCAGAGCCCCACTCTTTGACTCCAGTGGCTCAGGCA
  GATCCCCTTGTGAGAAGACAGCGAGTACAAGACCTTATGGCACAAATGCAGGGTCCCTATAATTTCATAC
  AGGATTCAATGCTGGATTTTGAAAATCAGACACTTGATCCTGCCATTGTATCTGCACAGCCTATGAATCC
  AACACAAAACATGGACATGCCCCAGCTGGTTTGCCCTCCAGTTCATTCTGAATCTAGACTTGCTCAGCCT
  AATCAAGTTCCTGTACAACCAGAAGCGACACAGGTTCCTTTGGTATCATCCACAAGTGAGGGGTACACAG
  CATCTCAACCCTTGTACCAGCCTTCTCATGCTACAGAGCAACGACCACAGAAGGAACCAATTGATCAGAT
  TCAGGCAACAATCTCTTTAAATACAGACCAGACTACAGCATCATCATCCCTTCCTGCTGCGTCTCAGCCT
  CAAGTATTTCAGGCTGGGACAAGCAAACCTTTACATAGCAGTGGAATCAATGTAAATGCAGCTCCATTCC
  AATCCATGCAAACGGTGTTCAATATGAATGCCCCAGTTCCTCCTGTTAATGAACCAGAAACTTTAAAACA
  GCAAAATCAGTACCAGGCCAGTTATAACCAGAGCTTTTCTAGTCAGCCTCACCAAGTAGAACAAACAGAG
  CTTCAGCAAGAACAGCTTCAAACAGTGGTTGGCACTTACCATGGTTCCCCAGACCAGTCCCATCAAGTGA
  CTGGTAACCACCAGCAGCCTCCTCAGCAGAACACTGGATTTCCACGTAGCAATCAGCCCTATTACAATAG
  TCGTGGTGTGTCTCGTGGAGGCTCCCGTGGTGCTAGAGGCTTGATGAATGGATACCGGGGCCCTGCCAAT
  GGATTCAGAGGAGGATATGATGGTTACCGCCCTTCATTCTCTAACACTCCAAACAGTGGTTATACACAGT
  CTCAGTTCAGTGCTCCCCGGGATTACTCTGGCTATCAACGGGATGGATATCAGCAGAATTTCAAGCGAGG
  CTCTGGGCAGAGTGGACCACGGGGAGCCCCACGAGGTCGTGGAGGGCCCCCAAGACCCAACAGAGGGATG
  CCGCAAATGAACACTCAGCAAGTGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200300 representing NM_005898
  Red=Cloning site Green=Tags(s)
  
  MPSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATGTGAVQTEAMKQILGVIDKKLRNLEK
  KKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTNNLEFAKELQRSFMALSQDIQKTIKKTARREQLMREE
  AEQKRLKTVLELQYVLDKLGDDEVRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHA
  SIHLWDLLEGKEKPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPA
  EEYTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPEPHSLTPVAQA
  DPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQNMDMPQLVCPPVHSESRLAQP
  NQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQRPQKEPIDQIQATISLNTDQTTASSSLPAASQP
  QVFQAGTSKPLHSSGINVNAAPFQSMQTVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTE
  LQQEQLQTVVGTYHGSPDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPAN
  GFRGGYDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPPRPNRGM
  PQMNTQQVN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005898
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005898.5
• RefSeq Size: 5562 bp
• RefSeq ORF: 2130 bp
• Locus ID: 4076
• UniProt ID: Q14444
• Cytogenetics: 11p13
• Gene Summary: May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Binds directly and selectively to MYC and CCND2 RNAs. In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs.[UniProtKB/Swiss-Prot Function]