C20orf31 (EDEM2) (NM_018217) Human Tagged ORF Clone

CAT#: RG200124

EDEM2 (tGFP-tagged) - Human ER degradation enhancer, mannosidase alpha-like 2 (EDEM2), transcript variant 1

Product Images

Circular map for RG200124

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C20orf31
• Synonyms: bA4204.1; C20orf31; C20orf49
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200124 representing NM_018217
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTTCCGGCTGCTCATCCCGCTCGGCCTCCTGTGCGCGCTGCTGCCTCAGCACCATGGTGCGCCAG
  GTCCCGACGGCTCCGCGCCAGATCCCGCCCACTACAGGGAGCGAGTCAAGGCCATGTTCTACCACGCCTA
  CGACAGCTACCTGGAGAATGCCTTTCCCTTCGATGAGCTGCGACCTCTCACCTGTGACGGGCACGACACC
  TGGGGCAGTTTTTCTCTGACTCTAATTGATGCACTGGACACCTTGCTGATTTTGGGGAATGTCTCAGAAT
  TCCAAAGAGTGGTTGAAGTGCTCCAGGACAGCGTGGACTTTGATATTGATGTGAACGCCTCTGTGTTTGA
  AACAAACATTCGAGTGGTAGGAGGACTCCTGTCTGCTCATCTGCTCTCCAAGAAGGCTGGGGTGGAAGTA
  GAGGCTGGATGGCCCTGTTCCGGGCCTCTCCTGAGAATGGCTGAGGAGGCGGCCCGAAAACTCCTCCCAG
  CCTTTCAGACCCCCACTGGCATGCCATATGGAACAGTGAACTTACTTCATGGCGTGAACCCAGGAGAGAC
  CCCTGTCACCTGTACGGCAGGGATTGGGACCTTCATTGTTGAATTTGCCACCCTGAGCAGCCTCACTGGT
  GACCCGGTGTTCGAAGATGTGGCCAGAGTGGCTTTGATGCGCCTCTGGGAGAGCCGGTCAGATATCGGGC
  TGGTCGGCAACCACATTGATGTGCTCACTGGCAAGTGGGTGGCCCAGGACGCAGGCATCGGGGCTGGCGT
  GGACTCCTACTTTGAGTACTTGGTGAAAGGAGCCATCCTGCTTCAGGATAAGAAGCTCATGGCCATGTTC
  CTAGAGTATAACAAAGCCATCCGGAACTACACCCGCTTCGATGACTGGTACCTGTGGGTTCAGATGTACA
  AGGGGACTGTGTCCATGCCAGTCTTCCAGTCCTTGGAGGCCTACTGGCCTGGTCTTCAGAGCCTCATTGG
  AGACATTGACAATGCCATGAGGACCTTCCTCAACTACTACACTGTATGGAAGCAGTTTGGGGGGCTCCCG
  GAATTCTACAACATTCCTCAGGGATACACAGTGGAGAAGCGAGAGGGCTACCCACTTCGGCCAGAACTTA
  TTGAAAGCGCAATGTACCTCTACCGTGCCACGGGGGATCCCACCCTCCTAGAACTCGGAAGAGATGCTGT
  GGAATCCATTGAAAAAATCAGCAAGGTGGAGTGCGGATTTGCAACAATCAAAGATCTGCGAGACCACAAG
  CTGGACAACCGCATGGAGTCGTTCTTCCTGGCCGAGACTGTGAAATACCTCTACCTCCTGTTTGACCCAA
  CCAACTTCATCCACAACAATGGGTCCACCTTCGACACGGTGATCACCCCCTATGGGGAGTGCATCCTGGG
  GGCTGGGGGGTACATCTTCAACACAGAAGCTCACCCCATCGACCCTGCCGCCCTGCACTGCTGCCAGAGG
  CTGAAGGAAGAGCAGTGGGAGGTGGAGGACTTGATGAGGGAATTCTACTCTCTCAAACGGAGCAGGTCGA
  AATTTCAGAAAAACACTGTTAGTTCGGGGCCATGGGAACCTCCAGCAAGGCCAGGAACACTCTTCTCACC
  AGAAAACCATGACCAGGCAAGGGAGAGGAAGCCTGCCAAACAGAAGGTCCCACTTCTCAGCTGCCCCAGT
  CAGCCCTTCACCTCCAAGTTGGCATTACTGGGACAGGTTTTCCTAGACTCCTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200124 representing NM_018217
  Red=Cloning site Green=Tags(s)
  
  MPFRLLIPLGLLCALLPQHHGAPGPDGSAPDPAHYRERVKAMFYHAYDSYLENAFPFDELRPLTCDGHDT
  WGSFSLTLIDALDTLLILGNVSEFQRVVEVLQDSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEV
  EAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTG
  DPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKLMAMF
  LEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMRTFLNYYTVWKQFGGLP
  EFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHK
  LDNRMESFFLAETVKYLYLLFDPTNFIHNNGSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQR
  LKEEQWEVEDLMREFYSLKRSRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPS
  QPFTSKLALLGQVFLDSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018217
• ORF Size: 1734 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018217.1, NP_060687.1
• RefSeq Size: 1885 bp
• RefSeq ORF: 1737 bp
• Locus ID: 55741
• UniProt ID: Q9BV94
• Cytogenetics: 20q11.22
• Domains: Glyco_hydro_47
• Protein Families: Secreted Protein
• Gene Summary: In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM, Mar 2008]