alpha 1 Catenin (CTNNA1) (NM_001290309) Human Tagged ORF Clone

CAT#: RC239595

CTNNA1 (myc-DDK-tagged) - Human catenin (cadherin-associated protein), alpha 1, 102kDa (CTNNA1), transcript variant 3

Product Images

Circular map for RC239595

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: alpha 1 Catenin
• Synonyms: CAP102; MDPT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239595 representing NM_001290309
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCTGCTGCAGGAGAGTTCGCAGATGATCCCTGCTCTTCTGTGAAGCGAGGCAACATGGTTCGGG
  CAGCTCGAGCTTTGCTCTCTGCTGTTACCCGGTTGCTGATTTTGGCTGACATGGCAGATGTCTACAAATT
  ACTTGTTCAGCTGAAAGTTGTGGAAGATGGTATCTTGAAGTTGAGGAATGCTGGCAATGAACAAGACTTA
  GGAATCCAGTATAAAGCCCTAAAACCTGAAGTGGATAAGCTGAACATTATGGCAGCCAAAAGACAACAGG
  AATTGAAAGATGTTGGCCATCGTGATCAGATGGCTGCAGCTAGAGGAATCCTGCAGAAGAACGTTCCGAT
  CCTCTATACTGCATCCCAGGCATGCCTACAGCACCCTGATGTCGCAGCCTATAAGGCCAACAGGGACCTG
  ATATACAAGCAGCTGCAGCAGGCGGTCACAGGCATTTCCAATGCAGCCCAGGCCACTGCCTCAGACGATG
  CCTCACAGCACCAGGGTGGAGGAGGAGGAGAACTGGCATATGCACTCAATAACTTTGACAAACAAATCAT
  TGTGGACCCCTTGAGCTTCAGCGAGGAGCGCTTTAGGCCTTCCCTGGAGGAGCGTCTGGAAAGCATCATT
  AGTGGGGCTGCCTTGATGGCCGACTCGTCCTGCACGCGTGATGACCGTCGTGAGCGAATTGTGGCAGAGT
  GTAATGCTGTCCGCCAGGCCCTGCAGGACCTGCTTTCGGAGTACATGGGCAATGCTGGACGTAAAGAAAG
  AAGTGATGCACTCAATTCTGCAATAGATAAAATGACCAAGAAGACCAGGGACTTGCGTAGACAGCTCCGC
  AAAGCTGTCATGGACCACGTTTCAGATTCTTTCCTGGAAACCAATGTTCCACTTTTGGTATTGATTGAAG
  CTGCAAAGAATGGAAATGAGAAAGAAGTTAAGGAGTATGCCCAAGTTTTCCGTGAACATGCCAACAAATT
  GATTGAGGTTGCCAACTTGGCCTGTTCCATCTCAAATAATGAAGAAGGTGTAAAGCTTGTTCGAATGTCT
  GCAAGCCAGTTAGAAGCCCTCTGTCCTCAGGTTATTAATGCTGCACTGGCTTTAGCAGCAAAACCACAGA
  GTAAACTGGCCCAAGAGAACATGGATCTTTTTAAAGAACAATGGGAAAAACAAGTCCGTGTTCTCACAGA
  TGCTGTCGATGACATTACTTCCATTGATGACTTCTTGGCTGTCTCAGAGAATCACATTTTGGAAGATGTG
  AACAAATGTGTCATTGCTCTCCAAGAGAAGGATGTGGATGGCCTGGACCGCACAGCTGGTGCAATTCGAG
  GCCGGGCAGCCCGGGTCATTCACGTAGTCACCTCAGAGATGGACAACTATGAGCCAGGAGTCTACACAGA
  GAAGGTTCTGGAAGCCACTAAGCTGCTCTCCAACACAGTCATGCCACGTTTTACTGAGCAAGTAGAAGCA
  GCCGTGGAAGCCCTCAGCTCGGACCCTGCCCAGCCCATGGATGAGAATGAGTTTATCGATGCTTCCCGCC
  TGGTATATGATGGCATCCGGGACATCAGGAAAGCAGTGCTGATGATAAGGACCCCTGAGGAGTTGGATGA
  CTCTGACTTTGAGACAGAAGATTTTGATGTCAGAAGCAGGACGAGCGTCCAGACAGAAGACGATCAGCTG
  ATAGCTGGCCAGAGTGCCCGGGCGATCATGGCTCAGCTTCCCCAGGAGCAAAAAGCGAAGATTGCGGAAC
  AGGTGGCCAGCTTCCAGGAAGAAAAGAGCAAGCTGGATGCTGAAGTGTCCAAATGGGACGACAGTGGCAA
  TGACATCATTGTGCTGGCCAAGCAGATGTGCATGATTATGATGGAGATGACAGACTTTACCCGAGGTAAA
  GGACCACTCAAAAATACATCGGATGTCATCAGTGCTGCCAAGAAAATTGCTGAGGCAGGATCCAGGATGG
  ACAAGCTTGGCCGCACCATTGCAGACCATTGCCCCGACTCGGCTTGCAAGCAGGACCTGCTGGCCTACCT
  GCAACGCATCGCCCTCTACTGCCACCAGCTGAACATCTGCAGCAAGGTCAAGGCCGAGGTGCAGAATCTC
  GGCGGGGAGCTTGTTGTCTCTGGGGTGGACAGCGCCATGTCCCTGATCCAGGCAGCCAAGAACTTGATGA
  ATGCTGTGGTGCAGACAGTGAAGGCATCCTACGTCGCCTCTACCAAATACCAAAAGTCACAGGGTATGGC
  TTCCCTCAACCTTCCTGCTGTGTCATGGAAGATGAAGGCACCAGAGAAAAAGCCATTGGTGAAGAGAGAG
  AAACAGGATGAGACACAGACCAAGATTAAACGGGCATCTCAGAAGAAGCACGTGAACCCGGTGCAGGCCC
  TCAGCGAGTTCAAAGCTATGGACAGCATC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239595 representing NM_001290309
  Red=Cloning site Green=Tags(s)
  
  MKAAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLILADMADVYKLLVQLKVVEDGILKLRNAGNEQDL
  GIQYKALKPEVDKLNIMAAKRQQELKDVGHRDQMAAARGILQKNVPILYTASQACLQHPDVAAYKANRDL
  IYKQLQQAVTGISNAAQATASDDASQHQGGGGGELAYALNNFDKQIIVDPLSFSEERFRPSLEERLESII
  SGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLR
  KAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQVFREHANKLIEVANLACSISNNEEGVKLVRMS
  ASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDV
  NKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEA
  AVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQL
  IAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGK
  GPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNL
  GGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKRE
  KQDETQTKIKRASQKKHVNPVQALSEFKAMDSI
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290309
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290309.3
• RefSeq Size: 3595 bp
• RefSeq ORF: 2412 bp
• Locus ID: 1495
• UniProt ID: P35221
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Endometrial cancer, Leukocyte transendothelial migration, Pathways in cancer, Tight junction
• MW: 89.1 kDa
• Gene Summary: This gene encodes a member of the catenin family of proteins that play an important role in cell adhesion process by connecting cadherins located on the plasma membrane to the actin filaments inside the cell. The encoded mechanosensing protein contains three vinculin homology domains and undergoes conformational changes in response to cytoskeletal tension, resulting in the reconfiguration of cadherin-actin filament connections. Certain mutations in this gene cause butterfly-shaped pigment dystrophy. [provided by RefSeq, May 2016]