DRP1 (DNM1L) (NM_001278465) Human Tagged ORF Clone

CAT#: RC239452

DNM1L (myc-DDK-tagged) - Human dynamin 1-like (DNM1L), transcript variant 6

Product Images

Circular map for RC239452

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DRP1
• Synonyms: DLP1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; OPA5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239452 representing NM_001278465
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCGCTAATTCCTGTCATAAACAAGCTCCAGGACGTCTTCAACACGGTGGGCGCCGACATCATCC
  AGCTGCCTCAAATCGTCGTAGTGGGAACGCAGAGCAGCGGAAAGAGCTCAGTGCTAGAAAGCCTGGTGGG
  GAGGGACCTGCTTCCCAGAGGTACTGGAATTGTCACCCGGAGACCTCTCATTCTGCAACTGGTCCATGTT
  TCACAAGAAGATAAACGGAAAACAACAGGAGAAGAAAATGACCCTGCTACATGGAAAAACTCAAGACACC
  TTTCTAAAGGGGTGGAAGCAGAAGAATGGGGTAAATTTCTTCACACCAAAAATAAGCTTTACACGGATTT
  TGATGAAATTCGACAAGAAATTGAAAATGAAACAGAAAGAATTTCAGGAAATAATAAGGGAGTAAGCCCT
  GAACCAATTCATCTTAAGATTTTTTCACCCAACGTTGTCAATTTGACACTTGTGGATTTGCCAGGAATGA
  CCAAGGTGCCTGTAGGTGATCAACCTAAGGATATTGAGCTTCAAATCAGAGAGCTCATTCTTCGGTTCAT
  CAGTAATCCTAATTCCATTATCCTCGCTGTCACTGCTGCTAATACAGATATGGCAACATCAGAGGCACTT
  AAAATTTCAAGAGAGGTAGATCCAGATGGTCGCAGAACCCTAGCTGTAATCACTAAACTTGATCTCATGG
  ATGCGGGTACTGATGCCATGGATGTATTGATGGGAAGGGTTATTCCAGTCAAACTTGGAATAATTGGAGT
  AGTTAACAGGAGCCAGCTAGATATTAACAACAAGAAGAGTGTAACTGATTCAATCCGTGATGAGTATGCT
  TTTCTTCAAAAGAAATATCCATCTCTGGCCAATAGAAATGGAACAAAGTATCTTGCTAGGACTCTAAACA
  GGTTACTGATGCATCACATCAGAGATTGTTTACCAGAGTTGAAAACAAGAATAAATGTTCTAGCTGCTCA
  GTATCAGTCTCTTCTAAATAGCTACGGTGAACCCGTGGATGATAAAAGTGCTACTTTACTCCAACTTATT
  ACCAAATTTGCCACAGAATATTGTAACACTATTGAAGGAACTGCAAAATATATTGAAACTTCGGAGCTAT
  GCGGTGGTGCTAGAATTTGTTATATTTTCCATGAGACTTTTGGGCGAACCTTAGAATCTGTTGATCCACT
  TGGTGGCCTTAACACTATTGACATTTTGACTGCCATTAGAAATGCTACTGGTCCTCGTCCTGCTTTATTT
  GTGCCTGAGGTTTCATTTGAGTTACTGGTGAAGCGGCAAATCAAACGTCTAGAAGAGCCCAGCCTCCGCT
  GTGTGGAACTGGTTCATGAGGAAATGCAAAGGATCATTCAGCACTGTAGCAATTACAGTACACAGGAATT
  GTTACGATTTCCTAAACTTCATGATGCCATAGTTGAAGTGGTGACTTGTCTTCTTCGTAAAAGGTTGCCT
  GTTACAAATGAAATGGTCCATAACTTAGTGGCAATTGAACTGGCTTATATCAACACAAAACATCCAGACT
  TTGCTGATGCTTGTGGGCTAATGAACAATAATATAGAGGAACAAAGGAGAAACAGGCTAGCCAGAGAATT
  ACCTTCAGCTGTATCACGAGACAAGTCTTCTAAAGTTCCAAGTGCTTTGGCACCTGCCTCCCAGGAGCCC
  TCCCCCGCTGCTTCTGCTGAGGCTGATGGCAAGGTTGCATCTGGAGGTGGTGGGGTTGGAGATGGTGTTC
  AAGAACCAACCACAGGCAACTGGAGAGGAATGCTGAAAACTTCAAAAGCTGAAGAGTTATTAGCAGAAGA
  AAAATCAAAACCCATTCCAATTATGCCAGCCAGTCCACAAAAAGGTCATGCCGTGAACCTGCTAGATGTG
  CCAGTTCCTGTTGCACGAAAACTATCTGCTCGGGAACAGCGAGATTGTGAGGTTATTGAACGACTCATTA
  AATCATATTTTCTCATTGTCAGAAAGAATATTCAAGACAGTGTGCCAAAGGCAGTAATGCATTTTTTGGT
  TAATCATGTGAAAGACACTCTTCAGAGTGAGCTAGTAGGCCAGCTGTATAAATCATCCTTATTGGATGAT
  CTTCTGACAGAATCTGAGGACATGGCACAGCGCAGGAAAGAAGCAGCTGATATGCTAAAGGCATTACAAG
  GAGCCAGTCAAATTATTGCTGAAATCCGGGAGACTCATCTTTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239452 representing NM_001278465
  Red=Cloning site Green=Tags(s)
  
  MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHV
  SQEDKRKTTGEENDPATWKNSRHLSKGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSP
  EPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEAL
  KISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYA
  FLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLI
  TKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALF
  VPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLP
  VTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEP
  SPAASAEADGKVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDV
  PVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDD
  LLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278465
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278465.1, NP_001265394.1
• RefSeq Size: 4621 bp
• RefSeq ORF: 2217 bp
• Locus ID: 10059
• UniProt ID: O00429
• Cytogenetics: 12p11.21
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• MW: 82.5 kDa
• Gene Summary: This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]