ZNF133 (NM_001282995) Human Tagged ORF Clone

CAT#: RC239215

ZNF133 (myc-DDK-tagged) - Human zinc finger protein 133 (ZNF133), transcript variant 3

Product Images

Circular map for RC239215

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF133
• Synonyms: pHZ-13; pHZ-66; ZNF150
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC239215 representing NM_001282995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTCAGGGATGTGGCTGTGGATTTCACCCAGGATGAGTGGAGGCTGCTGAGCCCTGCTCAAAGGA
  CTCTGTACAGAGAGGTGATGCTGGAGAACTACAGCAACCTGGTCTCACTGGGAATTTCATTTTCTAAACC
  AGAACTCATCACCCAGCTGGAGCAAGGGAAAGAGACCTGGAGAGAGGAAAAAAAATGTTCACCGGCAACC
  TGTCCAGATCCAGAGCCAGAGCTCTACCTCGATCCTTTCTGCCCTCCGGGTTTCTCCAGTCAGAAATTCC
  CCATGCAGCATGTGCTGTGTAATCATCCCCCCTGGATCTTCACATGCTTGTGTGCAGAAGGTAACATCCA
  GCCTGGGGATCCAGGCCCAGGGGACCAGGAGAAGCAGCAACAAGCCTCTGAGGGGAGACCCTGGAGTGAT
  CAAGCAGAAGGTCCTGAGGGAGAAGGTGCCATGCCTTTGTTTGGAAGAACCAAGAAAAGGACTCTGGGAG
  CGTTCTCCAGGCCACCCCAGAGGCAGCCAGTCAGCTCTCGGAACGGCCTCAGAGGGGTGGAGTTAGAAGC
  CAGCCCAGCTCAGACAGGGAACCCTGAGGAAACAGACAAATTGTTGAAGAGGATAGAAGTCTTAGGATTT
  GGAACAGTCAACTGTGGAGAGTGTGGACTGAGCTTCAGCAAGATGACAAACCTGCTCAGTCACCAGCGGA
  TACACTCAGGGGAGAAGCCCTACGTGTGTGGGGTATGTGAGAAGGGCTTCAGCCTAAAGAAGAGCCTCGC
  CAGACACCAGAAGGCACACTCGGGGGAGAAGCCAATTGTGTGCAGGGAGTGTGGACGAGGCTTTAACCGG
  AAGTCAACGCTAATCATACACGAACGGACACACTCCGGTGAGAAACCTTACATGTGCAGTGAGTGTGGGC
  GAGGCTTCAGCCAGAAGTCAAACCTCATCATACACCAGAGGACACACTCAGGGGAAAAGCCTTATGTGTG
  CCGGGAATGTGGCAAAGGCTTCAGCCAGAAGTCAGCTGTCGTGAGACACCAGAGGACACACTTGGAGGAG
  AAGACCATCGTGTGCAGTGACTGTGGCCTGGGCTTCAGCGACAGGTCAAACCTCATCTCCCACCAGAGGA
  CGCACTCTGGGGAGAAGCCCTACGCCTGCAAGGAGTGTGGGCGATGCTTCAGGCAGAGGACCACCCTTGT
  CAACCACCAGAGGACACACTCAAAGGAGAAGCCCTATGTGTGCGGGGTGTGTGGGCACAGCTTCAGCCAG
  AATTCAACCCTCATCTCTCACAGGCGGACACACACTGGGGAGAAGCCGTATGTTTGTGGGGTGTGTGGGC
  GAGGCTTTAGTCTCAAGTCACACCTCAACAGACACCAGAACATACACTCAGGAGAGAAGCCCATTGTGTG
  CAAGGACTGTGGCCGGGGCTTCAGCCAGCAATCCAACCTCATCAGACACCAGAGGACGCACTCAGGCGAG
  AAGCCCATGGTGTGTGGGGAGTGCGGGCGAGGCTTCAGCCAGAAGTCAAACCTTGTTGCACACCAGAGGA
  CGCACTCAGGGGAGAGGCCGTATGTGTGCCGAGAGTGCGGGCGAGGCTTTAGCCACCAGGCCGGTCTCAT
  CAGGCACAAGCGGAAGCACTCGAGGGAGAAGCCCTACATGTGCAGGCAGTGTGGACTGGGCTTTGGCAAT
  AAGTCAGCTCTAATCACACACAAGCGGGCTCACTCGGAAGAGAAGCCTTGTGTGTGCAGAGAGTGTGGCC
  AAGGCTTTCTCCAAAAGTCACACCTCACCTTACATCAAATGACACATACGGGGGAGAAGCCATATGTGTG
  CAAGACGTGTGGGCGGGGCTTCAGCCTCAAGTCTCACCTCAGCAGACACAGGAAGACCACGTCTGTCCAC
  CACAGACTGCCAGTGCAGCCCGACCCTGAGCCGTGTGCAGGGCAACCTTCGGATTCCTTATACTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC239215 representing NM_001282995
  Red=Cloning site Green=Tags(s)
  
  MAFRDVAVDFTQDEWRLLSPAQRTLYREVMLENYSNLVSLGISFSKPELITQLEQGKETWREEKKCSPAT
  CPDPEPELYLDPFCPPGFSSQKFPMQHVLCNHPPWIFTCLCAEGNIQPGDPGPGDQEKQQQASEGRPWSD
  QAEGPEGEGAMPLFGRTKKRTLGAFSRPPQRQPVSSRNGLRGVELEASPAQTGNPEETDKLLKRIEVLGF
  GTVNCGECGLSFSKMTNLLSHQRIHSGEKPYVCGVCEKGFSLKKSLARHQKAHSGEKPIVCRECGRGFNR
  KSTLIIHERTHSGEKPYMCSECGRGFSQKSNLIIHQRTHSGEKPYVCRECGKGFSQKSAVVRHQRTHLEE
  KTIVCSDCGLGFSDRSNLISHQRTHSGEKPYACKECGRCFRQRTTLVNHQRTHSKEKPYVCGVCGHSFSQ
  NSTLISHRRTHTGEKPYVCGVCGRGFSLKSHLNRHQNIHSGEKPIVCKDCGRGFSQQSNLIRHQRTHSGE
  KPMVCGECGRGFSQKSNLVAHQRTHSGERPYVCRECGRGFSHQAGLIRHKRKHSREKPYMCRQCGLGFGN
  KSALITHKRAHSEEKPCVCRECGQGFLQKSHLTLHQMTHTGEKPYVCKTCGRGFSLKSHLSRHRKTTSVH
  HRLPVQPDPEPCAGQPSDSLYSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282995
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282995.1, NP_001269924.1
• RefSeq Size: 2736 bp
• RefSeq ORF: 1962 bp
• Locus ID: 7692
• UniProt ID: P52736
• Cytogenetics: 20p11.23
• Protein Families: Transcription Factors
• MW: 73.3 kDa
• Gene Summary: May be involved in transcriptional regulation as a repressor.[UniProtKB/Swiss-Prot Function]