FAM222B (NM_001288631) Human Tagged ORF Clone

CAT#: RC238912

FAM222B (myc-DDK-tagged) - Human family with sequence similarity 222, member B (FAM222B), transcript variant 3

Product Images

Circular map for RC238912

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM222B
• Synonyms: C17orf63
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238912 representing NM_001288631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCAAGCTTCCCCCTTGCCTCTGCTAAAAAAAACCTCAGAGTTGGTACTAACTTGGAACAAAGGG
  ACTCTTCTACTCATCAAGGGGACACTACACAGAAAATGAGAACTGCTCACTATCCTACCCCAGCCGAATT
  GGATGCGTATGCTAAGAAGGTCGCAAACAACCCACTGACTATAAAAATCTTCCCCAACAGTGTGAAGGTT
  CCCCAGCGGAAACACGTTCGTCGTACTGTGAACGGCCTCGACACATCAGCCCAGCGCTACAGCCCCTACC
  CGACACAGGCTGCCACCAAGGCAGGCCTGCTTGCCATTGTCAAAGTGCCAGCCAAAAGCATACTCAAGGA
  CTTTGACGGCACCCGAGCCCGGTTGCTCCCTGAGGCCATCATGAACCCCCCAGTGGCACCCTATGCTACT
  GTGGCACCCAGCACTTTAGCCCACCCCCAGGCCCAGGCTCTGGCCCGCCAGCAGGCCCTGCAGCATGCAC
  AGACCCTGGCCCATGCCCCTCCCCAGACGCTGCAGCACCCTCAGGGTATCCCGCCACCCCAGGCACTGTC
  CCACCCTCAGAGCCTCCAGCAGCCTCAGGGCCTGGGCCACCCTCAGCCCATGGCCCAAACCCAGGGCTTG
  GTCCACCCTCAGGCCCTGGCTCACCAGGGTCTCCAGCACCCCCACAATCCCTTGCTGCATGGAGGCCGGA
  AGATGCCAGACTCAGATGCCCCCCCGAATGTGACCGTGTCTACCTCAACTATCCCCCTTTCAATGGCGGC
  CACTCTGCAGCACAGCCAGCCTCCGGACCTGAGTAGCATCGTGCACCAGATCAACCAGTTTTGCCAGACG
  AGGGCAGGCATCAGCACTACCTCAGTGTGTGAGGGCCAGATCGCCAACCCCAGCCCCATTAGTCGCAGTC
  TGCTCATCAATGCAAGCACCCGGGTGTCGACCCACAGCGTCCCCACACCAATGCCTTCATGTGTGGTCAA
  TCCCATGGAGCACACCCACGCGGCCACCGCCGCGTTGCCTGCTGCAGGTCCTGTCAACCTGCCCACAGGC
  ATCTCTCGCGTCCCCACTGGCTACCCTAGCGACCTCAAGCCAGTCACCTGGAACCAGCACCAGCTGGCCC
  ACCTACAGCAGATGTGCAGCGAGGCTAGTGGGACGCCAGCCCCTGGCCTGACAGGCAAGCATGCGGCAGG
  ACGCGAGTTGGCAGGGCCTGGCTTTGTGGGCAAGGCCCCTGCCTACCCGCAGGAACTCTGCCTGGCGCAG
  TCCTTCCATCTGAAGCCACCCCTGGAAAAGCCGACCCCATCCCCACCAGTCAACGGCATGGCAGCCCCAT
  TGGCCTACCCCAATGGTCACTACTTCCAACCCCTGTGGAACAACATTCTGCCCACTCCCAATAGCGACAG
  CTCGGGGTCTCAGGACCTTGCCATGCCGTTCCACGGTGGGCAGCCCACAGGTGCACCCCTCGACTGTGCG
  GCAGCTCCCGGGGCCCACTACCGAGCAGGGACCGGGGGCGGTCCAGTGGCAAGCCAGAACAGCTTGATGC
  AAACAGTGGATTACCTAAGTGGGGATTTCCAACAGGCCTGCTTCCGAGAACAGAGCCTGGCCATGCTGAG
  CAAGGCCCACCGAGCCCCTGGCAACCGAGCCCCCGATCCCACAGAGAGTCGAAGTCTTCATATTCAGCAC
  CCAGGGTATAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238912 representing NM_001288631
  Red=Cloning site Green=Tags(s)
  
  MRSSFPLASAKKNLRVGTNLEQRDSSTHQGDTTQKMRTAHYPTPAELDAYAKKVANNPLTIKIFPNSVKV
  PQRKHVRRTVNGLDTSAQRYSPYPTQAATKAGLLAIVKVPAKSILKDFDGTRARLLPEAIMNPPVAPYAT
  VAPSTLAHPQAQALARQQALQHAQTLAHAPPQTLQHPQGIPPPQALSHPQSLQQPQGLGHPQPMAQTQGL
  VHPQALAHQGLQHPHNPLLHGGRKMPDSDAPPNVTVSTSTIPLSMAATLQHSQPPDLSSIVHQINQFCQT
  RAGISTTSVCEGQIANPSPISRSLLINASTRVSTHSVPTPMPSCVVNPMEHTHAATAALPAAGPVNLPTG
  ISRVPTGYPSDLKPVTWNQHQLAHLQQMCSEASGTPAPGLTGKHAAGRELAGPGFVGKAPAYPQELCLAQ
  SFHLKPPLEKPTPSPPVNGMAAPLAYPNGHYFQPLWNNILPTPNSDSSGSQDLAMPFHGGQPTGAPLDCA
  AAPGAHYRAGTGGGPVASQNSLMQTVDYLSGDFQQACFREQSLAMLSKAHRAPGNRAPDPTESRSLHIQH
  PGYR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001288631
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288631.2
• RefSeq Size: 4350 bp
• RefSeq ORF: 1695 bp
• Locus ID: 55731
• UniProt ID: Q8WU58
• Cytogenetics: 17q11.2
• MW: 60.3 kDa