PPWD1 (NM_001278929) Human Tagged ORF Clone

CAT#: RC238525

PPWD1 (myc-DDK-tagged) - Human peptidylprolyl isomerase domain and WD repeat containing 1 (PPWD1), transcript variant 4

Product Images

Circular map for RC238525

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPWD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238525 representing NM_001278929
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTGTTTGATGTAGTGAACTTTGACATGATCAACATGCTGAAACTTGGCTATTTTCCTGGACAGT
  GTGAGTGGATCTATTGCCCAGGGGATGCAATTTCTTCAGTTGCTGCTTCCGAAAAGAGTACAGGAAAAAT
  TTTCATTTATGATGGCCGAGGAGATAACCAGCCACTTCATATTTTTGACAAACTCCATACATCACCTCTT
  ACTCAGATACGGCTAAACCCAGTTTACAAAGCAGTAGTGTCTTCTGACAAATCTGGGATGATTGAATACT
  GGACTGGGCCTCCTCATGAATATAAATTCCCCAAAAATGTGAACTGGGAATATAAAACTGACACTGATTT
  ATATGAATTTGCCAAGTGTAAGGCTTATCCAACCAGCGTATGTTTTTCACCAGATGGGAAGAAAATAGCT
  ACTATTGGTTCTGATAGAAAAGTTAGAATTTTCAGATTTGTAACTGGAAAACTCATGAGAGTCTTTGATG
  AATCACTAAGCATGTTTACTGAACTGCAACAGATGAGGCAACAGTTACCAGACATGGAATTTGGCCGACG
  AATGGCTGTAGAACGTGAGTTGGAGAAGGTTGATGCTGTAAGATTAATTAATATAGTTTTTGATGAAACT
  GGACACTTCGTGCTGTATGGAACAATGCTGGGCATTAAAGTTATAAATGTAGAGACAAACCGGTGTGTGC
  GGATTTTAGGCAAACAAGAAAATATTAGAGTGATGCAATTGGCTTTGTTCCAGGGGATAGCCAAAAAGCA
  TCGTGCTGCAACTACTATAGAAATGAAAGCTTCTGAAAATCCTGTTCTTCAGAATATTCAAGCTGACCCA
  ACAATAGTCTGTACATCATTCAAAAAGAATAGATTTTATATGTTTACCAAACGAGAACCAGAAGATACGA
  AAAGTGCAGATTCTGATCGAGATGTTTTTAATGAGAAACCTTCTAAAGAAGAAGTCATGGCAGCTACTCA
  AGCTGAAGGACCTAAACGAGTTTCGGACAGTGCCATTATCCACACCAGCATGGGAGACATTCACACCAAA
  CTTTTTCCTGTTGAGTGCCCTAAGACAGTGGAAAACTTCTGTGTTCACAGCAGAAATGGTTATTATAATG
  GGCATACATTTCACCGTATAATTAAGGGCTTTATGATTCAGACTGGAGATCCAACAGGTACTGGTATGGG
  AGGAGAAAGCATATGGGGAGGAGAATTTGAAGATGAATTTCATTCAACATTACGACATGACAGGCCATAC
  ACACTCAGCATGGCTAACGCGGGATCAAATACTAATGGATCCCAGTTTTTCATAACGGTAGTACCAACGC
  CTTGGCTTGATAATAAGCATACAGTATTTGGACGAGTGACTAAAGGAATGGAAGTTGTACAGAGGATCTC
  CAACGTCAAAGTCAATCCCAAAACAGATAAGCCCTATGAGGATGTCAGCATCATAAATATTACTGTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC238525 representing NM_001278929
  Red=Cloning site Green=Tags(s)
  
  MKVFDVVNFDMINMLKLGYFPGQCEWIYCPGDAISSVAASEKSTGKIFIYDGRGDNQPLHIFDKLHTSPL
  TQIRLNPVYKAVVSSDKSGMIEYWTGPPHEYKFPKNVNWEYKTDTDLYEFAKCKAYPTSVCFSPDGKKIA
  TIGSDRKVRIFRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVERELEKVDAVRLINIVFDET
  GHFVLYGTMLGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIEMKASENPVLQNIQADP
  TIVCTSFKKNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRVSDSAIIHTSMGDIHTK
  LFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGGEFEDEFHSTLRHDRPY
  TLSMANAGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPKTDKPYEDVSIINITVK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001278929
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278929.1, NP_001265858.1
• RefSeq Size: 2076 bp
• RefSeq ORF: 1473 bp
• Locus ID: 23398
• UniProt ID: Q96BP3
• Cytogenetics: 5q12.3
• MW: 56.1 kDa
• Gene Summary: PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be involved in pre-mRNA splicing (PubMed:11991638).[UniProtKB/Swiss-Prot Function]