P2Y6 (P2RY6) (NM_001277208) Human Tagged ORF Clone

CAT#: RC238176

P2RY6 (myc-DDK-tagged) - Human pyrimidinergic receptor P2Y, G-protein coupled, 6 (P2RY6), transcript variant 9

Product Images

Circular map for RC238176

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2Y6
• Synonyms: P2Y6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC238176 representing NM_001277208.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAGTCAGCATTGGTGTAAAGGACTGGAGCTTCAGGGTTCTCGGGATTCGAGTCCTGGCCCTGCTGCT
  TGCCAGCTCTGTGCTGTGGCAAGTTACTTCCTCTCCCCAGGTCTCTCGGTTTCCTCATCTGCTGCCTCT
  CCAGACTTCTGCCAGAACATTGCACGCGACAGTTTCAGGCACAGAACTGACTGGCAGCAGGGGCTGCTC
  CACGAGTGGGAATTTGCTCCAGCACTTCACGGACTGCAAGCGAGGCACTTGCTAACTCTTGGCCTCCCT
  GAACATAGGAAACCCACCTGGGCAGCCATGGAATGGGACAATGGCACAGGCCAGGCTCTGGGCTTGCCA
  CCCACCACCTGTGTCTACCGCGAGAACTTCAAGCAACTGCTGCTGCCACCTGTGTATTCGGCGGTGCTG
  GCGGCTGGCCTGCCGCTGAACATCTGTGTCATTACCCAGATCTGCACGTCCCGCCGGGCCCTGACCCGC
  ACGGCCGTGTACACCCTAAACCTTGCTCTGGCTGACCTGCTATATGCCTGCTCCCTGCCCCTGCTCATC
  TACAACTATGCCCAAGGTGATCACTGGCCCTTTGGCGACTTCGCCTGCCGCCTGGTCCGCTTCCTCTTC
  TATGCCAACCTGCACGGCAGCATCCTCTTCCTCACCTGCATCAGCTTCCAGCGCTACCTGGGCATCTGC
  CACCCGCTGGCCCCCTGGCACAAACGTGGGGGCCGCCGGGCTGCCTGGCTAGTGTGTGTAGCCGTGTGG
  CTGGCCGTGACAACCCAGTGCCTGCCCACAGCCATCTTCGCTGCCACAGGCATCCAGCGTAACCGCACT
  GTCTGCTATGACCTCAGCCCGCCTGCCCTGGCCACCCACTATATGCCCTATGGCATGGCTCTCACTGTC
  ATCGGCTTCCTGCTGCCCTTTGCTGCCCTGCTGGCCTGCTACTGTCTCCTGGCCTGCCGCCTGTGCCGC
  CAGGATGGCCCGGCAGAGCCTGTGGCCCAGGAGCGGCGTGGCAAGGCGGCCCGCATGGCCGTGGTGGTG
  GCTGCTGCCTTTGCCATCAGCTTCCTGCCTTTTCACATCACCAAGACAGCCTACCTGGCAGTGCGCTCG
  ACGCCGGGCGTCCCCTGCACTGTATTGGAGGCCTTTGCAGCGGCCTACAAAGGCACGCGGCCGTTTGCC
  AGTGCCAACAGCGTGCTGGACCCCATCCTCTTCTACTTCACCCAGAAGAAGTTCCGCCGGCGACCACAT
  GAGCTCCTACAGAAACTCACAGCCAAATGGCAGAGGCAGGGTCGC
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
  Protein Sequence

  >Peptide sequence encoded by RC238176
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MSQHWCKGLELQGSRDSSPGPAACQLCAVASYFLSPGLSVSSSAASPDFCQNIARDSFRHRTDWQQGLL
  HEWEFAPALHGLQARHLLTLGLPEHRKPTWAAMEWDNGTGQALGLPPTTCVYRENFKQLLLPPVYSAVL
  AAGLPLNICVITQICTSRRALTRTAVYTLNLALADLLYACSLPLLIYNYAQGDHWPFGDFACRLVRFLF
  YANLHGSILFLTCISFQRYLGICHPLAPWHKRGGRRAAWLVCVAVWLAVTTQCLPTAIFAATGIQRNRT
  VCYDLSPPALATHYMPYGMALTVIGFLLPFAALLACYCLLACRLCRQDGPAEPVAQERRGKAARMAVVV
  AAAFAISFLPFHITKTAYLAVRSTPGVPCTVLEAFAAAYKGTRPFASANSVLDPILFYFTQKKFRRRPH
  ELLQKLTAKWQRQGR
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277208
• ORF Size: 1287 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277208.1, NP_001264137.1
• RefSeq Size: 2466 bp
• RefSeq ORF: 1290 bp
• Locus ID: 5031
• UniProt ID: Q15077
• Cytogenetics: 11q13.4
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 47.5 kDa
• Gene Summary: The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Mar 2013]