NDRG2 (NM_001282214) Human Tagged ORF Clone

CAT#: RC237690

NDRG2 (myc-DDK-tagged) - Human NDRG family member 2 (NDRG2), transcript variant 12

Product Images

Circular map for RC237690

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDRG2
• Synonyms: SYLD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC237690 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGC
  GGCCAAGACTCACTCTGTGGAGACACCATACGTCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCC
  AAACGCCCAGCGATCCTTACCTACCACGATGTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTTC
  AGTTCGAGGACATGCAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAAGA
  GGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATCTCTGGACCAGCTTGCAGACATGATCCCTTGC
  GTCCTGCAGTACCTAAATTTCTCTACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTGGCGA
  GATATGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTGATCCCAATGCCAAGGG
  TTGGATGGATTGGGCAGCCCACAAGCTAACAGGCCTCACCTCTTCCATTCCGGAGATGATCCTTGGACAT
  CTTTTCAGCCAGGAAGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATATCATTACACATG
  CACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAACAACCGCCGAGACCTGAACTTTGAGCG
  TGGAGGTGATATCACCCTCAGGTGTCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCATGAAGATGCA
  GTGGTGGAATGTAACTCAAAACTGGACCCCACCCAGACCTCGTTCCTCAAGATGGCTGACTCCGGAGGTC
  AGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGAGGCCTTCAAGTACTTCCTGCAAGGCATGGGCTACAT
  GGCCTCATCCTGCATGACTCGCCTGTCCCGGTCTCGTACAGCCTCTCTGACCAGTGCAGCATCCGTTGAT
  GGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGCGAGTCTGGAACTCTTTCTTCGGGGCCCCCGG
  GGCACACCATGGAGGTCTCCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC237690 protein sequence
  Red=Cloning site Green=Tags(s)
  
  XWRSCRRCRSQRRSHCCQDRRLRRPRLTLWRHHTSLSLSLSMAPPNPNAQRSLPTTMWDSTINLASSHCF
  SSRTCRKSFRTLCGFMWMPLEWKREPLCSLWDISTHLWTSLQT*SLASCST*ISLQ*LELVLELEPTSWR
  DMLLTTRTLLKVLSSSTLIPMPRVGWIGQPTS*QASPLPFRR*SLDIFSARKSSLEILS*YKSTEISLHM
  HPTWITLNCTGTATTTAET*TLSVEVISPSGVL*CWW*ETKHLMKMQWWNVTQNWTPPRPRSSRWLTPEV
  SPS*LSQAS*PRPSSTSCKAWATWPHPA*LACPGLVQPL*PVQHPLMATGPALAPCPRAASLELFLRGPR
  GTPWRSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001282214
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282214.1, NP_001269143.1
• RefSeq Size: 2245 bp
• RefSeq ORF: 1074 bp
• Locus ID: 57447
• UniProt ID: Q9UN36
• Cytogenetics: 14q11.2
• MW: 39.3 kDa
• Gene Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]