CACNA1G (NM_001256332) Human Tagged ORF Clone

CAT#: RC235370

CACNA1G (Myc-DDK tagged) - Homo sapiens calcium channel, voltage-dependent, T type, alpha 1G subunit (CACNA1G), transcript variant 24

Product Images

Circular map for RC235370

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACNA1G
• Synonyms: Ca(V)T.1; Cav3.1; NBR13; SCA42; SCA42ND
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235370 representing NM_001256332
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGGAGGAGGATGGAGCGGGCGCCGAGGAGTCGGGACAGCCCCGGAGCTTCATGCGGCTCAACG
  ACCTGTCGGGGGCCGGGGGCCGGCCGGGGCCGGGGTCAGCAGAAAAGGACCCGGGCAGCGCGGACTCCGA
  GGCGGAGGGGCTGCCGTACCCGGCGCTGGCCCCGGTGGTTTTCTTCTACTTGAGCCAGGACAGCCGCCCG
  CGGAGCTGGTGTCTCCGCACGGTCTGTAACCCCTGGTTTGAGCGCATCAGCATGTTGGTCATCCTTCTCA
  ACTGCGTGACCCTGGGCATGTTCCGGCCATGCGAGGACATCGCCTGTGACTCCCAGCGCTGCCGGATCCT
  GCAGGCCTTTGATGACTTCATCTTTGCCTTCTTTGCCGTGGAGATGGTGGTGAAGATGGTGGCCTTGGGC
  ATCTTTGGGAAAAAGTGTTACCTGGGAGACACTTGGAACCGGCTTGACTTTTTCATCGTCATCGCAGGGA
  TGCTGGAGTACTCGCTGGACCTGCAGAACGTCAGCTTCTCAGCTGTCAGGACAGTCCGTGTGCTGCGACC
  GCTCAGGGCCATTAACCGGGTGCCCAGCATGCGCATCCTTGTCACGTTGCTGCTGGATACGCTGCCCATG
  CTGGGCAACGTCCTGCTGCTCTGCTTCTTCGTCTTCTTCATCTTCGGCATCGTCGGCGTCCAGCTGTGGG
  CAGGGCTGCTTCGGAACCGATGCTTCCTACCTGAGAATTTCAGCCTCCCCCTGAGCGTGGACCTGGAGCG
  CTATTACCAGACAGAGAACGAGGATGAGAGCCCCTTCATCTGCTCCCAGCCACGCGAGAACGGCATGCGG
  TCCTGCAGAAGCGTGCCCACGCTGCGCGGGGACGGGGGCGGTGGCCCACCTTGCGGTCTGGACTATGAGG
  CCTACAACAGCTCCAGCAACACCACCTGTGTCAACTGGAACCAGTACTACACCAACTGCTCAGCGGGGGA
  GCACAACCCCTTCAAGGGCGCCATCAACTTTGACAACATTGGCTATGCCTGGATCGCCATCTTCCAGGTC
  ATCACGCTGGAGGGCTGGGTCGACATCATGTACTTTGTGATGGATGCTCATTCCTTCTACAATTTCATCT
  ACTTCATCCTCCTCATCATCGTGGGCTCCTTCTTCATGATCAACCTGTGCCTGGTGGTGATTGCCACGCA
  GTTCTCAGAGACCAAGCAGCGGGAAAGCCAGCTGATGCGGGAGCAGCGTGTGCGGTTCCTGTCCAACGCC
  AGCACCCTGGCTAGCTTCTCTGAGCCCGGCAGCTGCTATGAGGAGCTGCTCAAGTACCTGGTGTACATCC
  TTCGTAAGGCAGCCCGCAGGCTGGCTCAGGTCTCTCGGGCAGCAGGTGTGCGGGTTGGGCTGCTCAGCAG
  CCCAGCACCCCTCGGGGGCCAGGAGACCCAGCCCAGCAGCAGCTGCTCTCGCTCCCACCGCCGCCTATCC
  GTCCACCACCTGGTGCACCACCACCACCACCATCACCACCACTACCACCTGGGCAATGGGACGCTCAGGG
  CCCCCCGGGCCAGCCCGGAGATCCAGGACAGGGATGCCAATGGGTCCCGCCGGCTCATGCTGCCACCACC
  CTCGACGCCTGCCCTCTCCGGGGCCCCCCCTGGTGGCGCAGAGTCTGTGCACAGCTTCTACCATGCCGAC
  TGCCACTTAGAGCCAGTCCGCTGCCAGGCGCCCCCTCCCAGGTCCCCATCTGAGGCATCCGGCAGGACTG
  TGGGCAGCGGGAAGGTGTATCCCACCGTGCACACCAGCCCTCCACCGGAGACGCTGAAGGAGAAGGCACT
  AGTAGAGGTGGCTGCCAGCTCTGGGCCCCCAACCCTCACCAGCCTCAACATCCCACCCGGGCCCTACAGC
  TCCATGCACAAGCTGCTGGAGACACAGAGTACAGGTGCCTGCCAAAGCTCTTGCAAGATCTCCAGCCCTT
  GCTTGAAAGCAGACAGTGGAGCCTGTGGTCCAGACAGCTGCCCCTACTGTGCCCGGGCCGGGGCAGGGGA
  GGTGGAGCTCGCCGACCGTGAAATGCCTGACTCAGACAGCGAGGCAGTTTATGAGTTCACACAGGATGCC
  CAGCACAGCGACCTCCGGGACCCCCACAGCCGGCGGCAACGGAGCCTGGGCCCAGATGCAGAGCCCAGCT
  CTGTGCTGGCCTTCTGGAGGCTAATCTGTGACACCTTCCGAAAGATTGTGGACAGCAAGTACTTTGGCCG
  GGGAATCATGATCGCCATCCTGGTCAACACACTCAGCATGGGCATCGAATACCACGAGCAGCCCGAGGAG
  CTTACCAACGCCCTAGAAATCAGCAACATCGTCTTCACCAGCCTCTTTGCCCTGGAGATGCTGCTGAAGC
  TGCTTGTGTATGGTCCCTTTGGCTACATCAAGAATCCCTACAACATCTTCGATGGTGTCATTGTGGTCAT
  CAGCGTGTGGGAGATCGTGGGCCAGCAGGGGGGCGGCCTGTCGGTGCTGCGGACCTTCCGCCTGATGCGT
  GTGCTGAAGCTGGTGCGCTTCCTGCCGGCGCTGCAGCGGCAGCTGGTGGTGCTCATGAAGACCATGGACA
  ACGTGGCCACCTTCTGCATGCTGCTTATGCTCTTCATCTTCATCTTCAGCATCCTGGGCATGCATCTCTT
  CGGCTGCAAGTTTGCCTCTGAGCGGGATGGGGACACCCTGCCAGACCGGAAGAATTTTGACTCCTTGCTC
  TGGGCCATCGTCACTGTCTTTCAGATCCTGACCCAGGAGGACTGGAACAAAGTCCTCTACAATGGTATGG
  CCTCCACGTCGTCCTGGGCGGCCCTTTATTTCATTGCCCTCATGACCTTCGGCAACTACGTGCTCTTCAA
  TTTGCTGGTCGCCATTCTGGTGGAGGGCTTCCAGGCGGAGGGAGATGCCAACAAGTCCGAATCAGAGCCC
  GATTTCTTCTCACCCAGCCTGGATGGTGATGGGGACAGGAAGAAGTGCTTGGCCTTGGTGTCCCTGGGAG
  AGCACCCGGAGCTGCGGAAGAGCCTGCTGCCGCCTCTCATCATCCACACGGCCGCCACACCCATGTCGCT
  GCCCAAGAGCACCAGCACGGGCCTGGGCGAGGCGCTGGGCCCTGCGTCGCGCCGCACCAGCAGCAGCGGG
  TCGGCAGAGCCTGGGGCGGCCCACGAGATGAAGTCACCGCCCAGCGCCCGCAGCTCTCCGCACAGCCCCT
  GGAGCGCTGCAAGCAGCTGGACCAGCAGGCGCTCCAGCCGGAACAGCCTCGGCCGTGCACCCAGCCTGAA
  GCGGAGAAGCCCAAGTGGAGAGCGGCGGTCCCTGTTGTCGGGAGAAGGCCAGGAGAGCCAGGATGAAGAG
  GAGAGCTCAGAAGAGGAGCGGGCCAGCCCTGCGGGCAGTGACCATCGCCACAGGGGGTCCCTGGAGCGGG
  AGGCCAAGAGTTCCTTTGACCTGCCAGACACACTGCAGGTGCCAGGGCTGCATCGCACTGCCAGTGGCCG
  AGGGTCTGCTTCTGAGCACCAGGACTGCAATGGCAAGTCGGCTTCAGGGCGCCTGGCCCGGGCCCTGCGG
  CCTGATGACCCCCCACTGGATGGGGATGACGCCGATGACGAGGGCAACCTGAGCAAAGGGGAACGGGTCC
  GCGCGTGGATCCGAGCCCGACTCCCTGCCTGCTGCCTCGAGCGAGACTCCTGGTCAGCCTACATCTTCCC
  TCCTCAGTCCAGGTTCCGCCTCCTGTGTCACCGGATCATCACCCACAAGATGTTCGACCACGTGGTCCTT
  GTCATCATCTTCCTTAACTGCATCACCATCGCCATGGAGCGCCCCAAAATTGACCCCCACAGCGCTGAAC
  GCATCTTCCTGACCCTCTCCAATTACATCTTCACCGCAGTCTTTCTGGCTGAAATGACAGTGAAGGTGGT
  GGCACTGGGCTGGTGCTTCGGGGAGCAGGCGTACCTGCGGAGCAGTTGGAACGTGCTGGACGGGCTGTTG
  GTGCTCATCTCCGTCATCGACATTCTGGTGTCCATGGTCTCTGACAGCGGCACCAAGATCCTGGGCATGC
  TGAGGGTGCTGCGGCTGCTGCGGACCCTGCGCCCGCTCAGGGTGATCAGCCGGGCGCAGGGGCTGAAGCT
  GGTGGTGGAGACGCTGATGTCCTCACTGAAACCCATCGGCAACATTGTAGTCATCTGCTGTGCCTTCTTC
  ATCATTTTCGGCATCTTGGGGGTGCAGCTCTTCAAAGGGAAGTTTTTCGTGTGCCAGGGCGAGGATACCA
  GGAACATCACCAATAAATCGGACTGTGCCGAGGCCAGTTACCGGTGGGTCCGGCACAAGTACAACTTTGA
  CAACCTTGGCCAGGCCCTGATGTCCCTGTTCGTTTTGGCCTCCAAGGATGGTTGGGTGGACATCATGTAC
  GATGGGCTGGATGCTGTGGGCGTGGACCAGCAGAACTTCCACAAGTGTCGGCAGCACCAGGAGGAAGAGG
  AGGCCCGGCGGCGGGAGGAGAAGCGCCTACGAAGACTGGAGAAAAAGAGAAGGAATCTAATGCTGGACGA
  TGTAATTGCTTCCGGCAGCTCAGCCAGCGCTGCGTCAGAAGCCCAGTGCAAACCTTACTACTCCGACTAC
  TCCCGCTTCCGGCTCCTCGTCCACCACTTGTGCACCAGCCACTACCTGGACCTCTTCATCACAGGTGTCA
  TCGGGCTGAACGTGGTCACCATGGCCATGGAGCACTACCAGCAGCCCCAGATTCTGGATGAGGCTCTGAA
  GATCTGCAACTACATCTTCACTGTCATCTTTGTCTTGGAGTCAGTTTTCAAACTTGTGGCCTTTGGTTTC
  CGTCGGTTCTTCCAGGACAGGTGGAACCAGCTGGACCTGGCCATTGTGCTGCTGTCCATCATGGGCATCA
  CGCTGGAGGAAATCGAGGTCAACGCCTCGCTGCCCATCAACCCCACCATCATCCGCATCATGAGGGTGCT
  GCGCATTGCCCGAGTGCTGAAGCTGCTGAAGATGGCTGTGGGCATGCGGGCGCTGCTGGACACGGTGATG
  CAGGCCCTGCCCCAGGTGGGGAACCTGGGACTTCTCTTCATGTTGTTGTTTTTCATCTTTGCAGCTCTGG
  GCGTGGAGCTCTTTGGAGACCTGGAGTGTGACGAGACACACCCCTGTGAGGGCCTGGGCCGTCATGCCAC
  CTTTCGGAACTTTGGCATGGCCTTCCTAACCCTCTTCCGAGTCTCCACAGGTGACAATTGGAATGGCATT
  ATGAAGGACACCCTCCGGGACTGTGACCAGGAGTCCACCTGCTACAACACGGTCATCTCGCCTATCTACT
  TTGTGTCCTTCGTGCTGACGGCCCAGTTCGTGCTAGTCAACGTGGTGATCGCCGTGCTGATGAAGCACCT
  GGAGGAGAGCAACAAGGAGGCCAAGGAGGAGGCCGAGCTAGAGGCTGAGCTGGAGCTGGAGATGAAGACC
  CTCAGCCCCCAGCCCCACTCGCCACTGGGCAGCCCCTTCCTCTGGCCTGGGGTCGAGGGCCCCGACAGCC
  CCGACAGCCCCAAGCCTGGGGCTCTGCACCCAGCGGCCCACGCGAGATCAGCCTCCCACTTTTCCCTGGA
  GCACCCCACGATGCAGCCCCACCCCACGGAGCTGCCAGGACCAGACTTACTGACTGTGCGGAAGTCTGGG
  GTCAGCCGAACGCACTCTCTGCCCAATGACAGCTACATGTGTCGGCATGGGAGCACTGCCGAGGGGCCCC
  TGGGACACAGGGGCTGGGGGCTCCCCAAAGCTCAGTCAGGCTCCGTCTTGTCCGTTCACTCCCAGCCAGC
  AGATACCAGCTACATCCTGCAGCTTCCCAAAGATGCACCTCATCTGCTCCAGCCCCACAGCGCCCCAACC
  TGGGGCACCATCCCCAAACTGCCCCCACCAGGACGCTCCCCTTTGGCTCAGAGGCCACTCAGGCGCCAGG
  CAGCAATAAGGACTGACTCCTTGGACGTTCAGGGTCTGGGCAGCCGGGAAGACCTGCTGGCAGAGGTGAG
  TGGGCCCTCCCCGCCCCTGGCCCGGGCCTACTCTTTCTGGGGCCAGTCAAGTACCCAGGCACAGCAGCAC
  TCCCGCAGCCACAGCAAGATCTCCAAGCACATGACCCCGCCAGCCCCTTGCCCAGGCCCAGAACCCAACT
  GGGGCAAGGGCCCTCCAGAGACCAGAAGCAGCTTAGAGTTGGACACGGAGCTGAGCTGGATTTCAGGAGA
  CCTCCTGCCCCCTGGCGGCCAGGAGGAGCCCCCATCCCCACGGGACCTGAAGAAGTGCTACAGCGTGGAG
  GCCCAGAGCTGCCAGCGCCGGCCTACGTCCTGGCTGGATGAGCAGAGGAGACACTCTATCGCCGTCAGCT
  GCCTGGACAGCGGCTCCCAACCCCACCTGGGCACAGACCCCTCTAACCTTGGGGGCCAGCCTCTTGGGGG
  GCCTGGGAGCCGGCCCAAGAAAAAACTCAGCCCGCCTAGTATCACCATAGACCCCCCCGAGAGCCAAGGT
  CCTCGGACCCCGCCCAGCCCTGGTATCTGCCTCCGGAGGAGGGCTCCGTCCAGCGACTCCAAGGATCCCT
  TGGCCTCTGGCCCCCCTGACAGCATGGCTGCCTCGCCCTCCCCAAAGAAAGATGTGCTGAGTCTCTCCGG
  TTTATCCTCTGACCCAGCAGACCTGGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235370 representing NM_001256332
  Red=Cloning site Green=Tags(s)
  
  MDEEEDGAGAEESGQPRSFMRLNDLSGAGGRPGPGSAEKDPGSADSEAEGLPYPALAPVVFFYLSQDSRP
  RSWCLRTVCNPWFERISMLVILLNCVTLGMFRPCEDIACDSQRCRILQAFDDFIFAFFAVEMVVKMVALG
  IFGKKCYLGDTWNRLDFFIVIAGMLEYSLDLQNVSFSAVRTVRVLRPLRAINRVPSMRILVTLLLDTLPM
  LGNVLLLCFFVFFIFGIVGVQLWAGLLRNRCFLPENFSLPLSVDLERYYQTENEDESPFICSQPRENGMR
  SCRSVPTLRGDGGGGPPCGLDYEAYNSSSNTTCVNWNQYYTNCSAGEHNPFKGAINFDNIGYAWIAIFQV
  ITLEGWVDIMYFVMDAHSFYNFIYFILLIIVGSFFMINLCLVVIATQFSETKQRESQLMREQRVRFLSNA
  STLASFSEPGSCYEELLKYLVYILRKAARRLAQVSRAAGVRVGLLSSPAPLGGQETQPSSSCSRSHRRLS
  VHHLVHHHHHHHHHYHLGNGTLRAPRASPEIQDRDANGSRRLMLPPPSTPALSGAPPGGAESVHSFYHAD
  CHLEPVRCQAPPPRSPSEASGRTVGSGKVYPTVHTSPPPETLKEKALVEVAASSGPPTLTSLNIPPGPYS
  SMHKLLETQSTGACQSSCKISSPCLKADSGACGPDSCPYCARAGAGEVELADREMPDSDSEAVYEFTQDA
  QHSDLRDPHSRRQRSLGPDAEPSSVLAFWRLICDTFRKIVDSKYFGRGIMIAILVNTLSMGIEYHEQPEE
  LTNALEISNIVFTSLFALEMLLKLLVYGPFGYIKNPYNIFDGVIVVISVWEIVGQQGGGLSVLRTFRLMR
  VLKLVRFLPALQRQLVVLMKTMDNVATFCMLLMLFIFIFSILGMHLFGCKFASERDGDTLPDRKNFDSLL
  WAIVTVFQILTQEDWNKVLYNGMASTSSWAALYFIALMTFGNYVLFNLLVAILVEGFQAEGDANKSESEP
  DFFSPSLDGDGDRKKCLALVSLGEHPELRKSLLPPLIIHTAATPMSLPKSTSTGLGEALGPASRRTSSSG
  SAEPGAAHEMKSPPSARSSPHSPWSAASSWTSRRSSRNSLGRAPSLKRRSPSGERRSLLSGEGQESQDEE
  ESSEEERASPAGSDHRHRGSLEREAKSSFDLPDTLQVPGLHRTASGRGSASEHQDCNGKSASGRLARALR
  PDDPPLDGDDADDEGNLSKGERVRAWIRARLPACCLERDSWSAYIFPPQSRFRLLCHRIITHKMFDHVVL
  VIIFLNCITIAMERPKIDPHSAERIFLTLSNYIFTAVFLAEMTVKVVALGWCFGEQAYLRSSWNVLDGLL
  VLISVIDILVSMVSDSGTKILGMLRVLRLLRTLRPLRVISRAQGLKLVVETLMSSLKPIGNIVVICCAFF
  IIFGILGVQLFKGKFFVCQGEDTRNITNKSDCAEASYRWVRHKYNFDNLGQALMSLFVLASKDGWVDIMY
  DGLDAVGVDQQNFHKCRQHQEEEEARRREEKRLRRLEKKRRNLMLDDVIASGSSASAASEAQCKPYYSDY
  SRFRLLVHHLCTSHYLDLFITGVIGLNVVTMAMEHYQQPQILDEALKICNYIFTVIFVLESVFKLVAFGF
  RRFFQDRWNQLDLAIVLLSIMGITLEEIEVNASLPINPTIIRIMRVLRIARVLKLLKMAVGMRALLDTVM
  QALPQVGNLGLLFMLLFFIFAALGVELFGDLECDETHPCEGLGRHATFRNFGMAFLTLFRVSTGDNWNGI
  MKDTLRDCDQESTCYNTVISPIYFVSFVLTAQFVLVNVVIAVLMKHLEESNKEAKEEAELEAELELEMKT
  LSPQPHSPLGSPFLWPGVEGPDSPDSPKPGALHPAAHARSASHFSLEHPTMQPHPTELPGPDLLTVRKSG
  VSRTHSLPNDSYMCRHGSTAEGPLGHRGWGLPKAQSGSVLSVHSQPADTSYILQLPKDAPHLLQPHSAPT
  WGTIPKLPPPGRSPLAQRPLRRQAAIRTDSLDVQGLGSREDLLAEVSGPSPPLARAYSFWGQSSTQAQQH
  SRSHSKISKHMTPPAPCPGPEPNWGKGPPETRSSLELDTELSWISGDLLPPGGQEEPPSPRDLKKCYSVE
  AQSCQRRPTSWLDEQRRHSIAVSCLDSGSQPHLGTDPSNLGGQPLGGPGSRPKKKLSPPSITIDPPESQG
  PRTPPSPGICLRRRAPSSDSKDPLASGPPDSMAASPSPKKDVLSLSGLSSDPADLDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256332
• ORF Size: 6681 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256332.2
• RefSeq Size: 7814 bp
• RefSeq ORF: 6684 bp
• Locus ID: 8913
• UniProt ID: O43497
• Cytogenetics: 17q21.33
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway, Type II diabetes mellitus
• MW: 246.7 kDa
• Gene Summary: Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes a T-type, low-voltage activated calcium channel. The T-type channels generate currents that are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2011]