KAT6B (NM_001256469) Human Tagged ORF Clone

CAT#: RC235335

KAT6B (Myc-DDK tagged) - Homo sapiens K(lysine) acetyltransferase 6B (KAT6B), transcript variant 3

Product Images

Circular map for RC235335

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KAT6B
• Synonyms: GTPTS; MORF; MOZ2; MYST4; qkf; querkopf; ZC2HC6B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235335 representing NM_001256469
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAAAACTTGCAAACCCACTTTATACAGAGTGGATTCTTGAAGCTATACAGAAAATAAAAAAGCAAA
  AGCAAAGGCCCTCTGAAGAGAGAATCTGCCATGCGGTCAGTACTTCCCATGGGTTGGATAAGAAGACAGT
  CTCTGAACAGCTGGAACTCAGTGTTCAGGATGGCTCAGTTCTCAAAGTCACCAACAAAGGCCTTGCCTCC
  TATAAGGACCCAGACAACCCTGGGCGCTTTTCATCAGTTAAACCAGGCACTTTTCCTAAGTCAGCCAAGG
  GGTCTAGAGGATCATGTAATGATCTCCGCAATGTGGATTGGAATAAACTTTTAAGGAGAGCAATTGAAGG
  ACTTGAGGAGCCGAATGGCTCCTCCCTGAAGAACATAGAGAAGTATCTCAGAAGTCAAAGTGATCTCACA
  AGCACCACCAACAACCCAGCCTTTCAGCAGCGGCTGCGACTGGGGGCCAAACGCGCTGTGAATAATGGGA
  GGTTACTGAAAGACGGACCGCAGTACAGGGTCAATTATGGGAGCTTAGATGGCAAAGGGGCACCTCAGTA
  TCCCAGTGCATTCCCATCCTCGCTCCCACCTGTCAGCCTTCTACCCCATGAGAAAGACCAGCCCCGTGCT
  GATCCCATTCCAATATGTAGCTTCTGTTTGGGGACTAAAGAATCAAATCGTGAAAAGAAACCAGAAGAAC
  TCCTCTCTTGTGCAGATTGTGGCAGTAGTGGACACCCATCCTGTTTGAAATTTTGTCCTGAATTAACAAC
  AAATGTAAAGGCCTTAAGGTGGCAGTGCATCGAATGCAAGACATGCAGTGCCTGTAGAGTCCAAGGCAGA
  AATGCTGATAATATGCTTTTTTGTGATTCCTGTGATAGAGGATTTCATATGGAATGCTGTGACCCACCAC
  TTTCCAGAATGCCAAAAGGGATGTGGATTTGCCAAGTCTGCAGACCAAAGAAAAAGGGAAGAAAACTACT
  TCATGAGAAAGCTGCACAAATAAAACGACGATATGCAAAACCCATTGGACGACCGAAAAATAAATTAAAG
  CAACGATTGTTGTCTGTAACCAGTGATGAAGGATCCATGAATGCATTCACAGGAAGGGGGTCACCTGATA
  CTGAAATAAAAATAAACATCAAACAAGAAAGTGCAGATGTAAATGTGATTGGAAACAAGGATGTCGTTAC
  TGAAGAGGATTTGGATGTTTTTAAGCAGGCCCAGGAACTTTCTTGGGAGAAAATAGAGTGTGAGAGTGGG
  GTGGAAGACTGTGGCCGGTACCCTTCTGTGATTGAATTTGGTAAATATGAAATCCAAACCTGGTACTCCT
  CGCCTTACCCACAGGAATATGCAAGATTACCAAAGCTTTACCTGTGTGAATTCTGTCTTAAATATATGAA
  AAGTAAAAATATTTTGCTAAGACACTCCAAGAAGTGTGGATGGTTTCATCCTCCAGCAAATGAAATTTAC
  CGAAGGAAAGACCTTTCAGTATTTGAGGTTGATGGGAATATGAGCAAAATTTATTGCCAAAACCTTTGCT
  TGTTAGCCAAGCTCTTCCTGGACCACAAAACGTTGTATTATGATGTCGAGCCATTCCTTTTTTATGTCCT
  TACAAAAAATGATGAAAAGGGCTGTCATCTGGTTGGATACTTCTCTAAGGAAAAGCTTTGCCAGCAGAAG
  TATAATGTCTCCTGCATAATGATCATGCCCCAGCACCAAAGGCAAGGATTTGGACGGTTTCTCATTGATT
  TCAGCTATTTGCTTTCTAGAAGAGAAGGCCAAGCAGGGTCTCCTGAAAAGCCTCTCTCCGATCTGGGCCG
  TCTCTCCTACCTGGCATATTGGAAGAGCGTCATCTTGGAGTATCTCTACCACCACCATGAGAGGCACATC
  AGCATCAAGGCAATTAGCAGAGCGACGGGCATGTGCCCACATGACATTGCCACCACTCTGCAGCACCTCC
  ACATGATCGACAAGAGAGATGGCAGATTTGTCATCATTAGACGGGAAAAGTTGATATTGAGCCACATGGA
  AAAGCTGAAAACCTGTTCCAGAGCCAATGAACTTGATCCAGACAGTCTGAGGTGGACCCCAATTTTAATT
  TCTAATGCTGCAGTGTCTGAAGAAGAGCGAGAAGCTGAGAAAGAGGCTGAGCGGCTAATGGAACAAGCTA
  GCTGCTGGGAGAAGGAGGAACAAGAAATCCTGTCAACTAGAGCTAACAGTAGGCAATCACCTGCAAAAGT
  ACAATCGAAAAATAAATATTTGCATTCCCCGGAGAGCCGGCCAGTCACAGGGGAGCGAGGGCAGCTGCTG
  GAGCTGTCTAAAGAGAGCAGTGAAGAAGAAGAGGAGGAGGAGGACGAGGAGGAGGAAGAAGAGGAGGAAG
  AAGAGGAAGAGGATGAAGAGGAGGAAGAAGAGGAAGAAGAAGAAGAAGAAGAAGAAAATATTCAAAGCTC
  TCCCCCAAGATTGACGAAACCACAGTCAGTTGCCATAAAGAGAAAGAGGCCTTTTGTACTAAAGAAGAAA
  AGGGGTCGTAAACGCAGGAGGATCAACAGCAGTGTAACAACAGAGACCATTTCAGAGACGACAGAAGTAC
  TGAATGAGCCCTTTGACAACTCAGATGAAGAGAGGCCAATGCCACAGCTGGAGCCTACCTGTGAGATTGA
  AGTGGAGGAAGATGGCAGGAAGCCAGTCCTGAGAAAAGCATTCCAGCATCAGCCTGGGAAGAAAAGACAA
  ACAGAGGAAGAGGAAGGAAAAGACAATCATTGCTTCAAGAATGCTGACCCTTGTAGAAACAATATGAATG
  ATGATTCAAGTAACTTGAAAGAAGGCAGTAAAGACAATCCCGAACCTCTAAAGTGCAAACAAGTGTGGCC
  AAAAGGAACAAAGCGCGGTCTATCTAAGTGGAGGCAAAACAAAGAGAGGAAGACCGGATTTAAACTGAAT
  TTGTACACCCCGCCAGAAACACCCATGGAGCCTGACGAGCAGGTAACAGTGGAAGAACAGAAGGAGACTT
  CAGAAGGAAAAACCAGCCCCAGTCCCATCAGGATTGAGGAGGAGGTCAAGGAAACTGGGGAAGCCCTGTT
  GCCTCAAGAGGAAAACAGAAGGGAAGAAACATGTGCCCCTGTAAGTCCAAACACATCACCAGGTGAAAAA
  CCAGAAGATGATCTCATCAAACCTGAGGAAGAGGAAGAGGAGGAGGAGGAGGAAGAGGAAGAAGAGGAAG
  AAGAGGAAGGGGAAGAAGAAGAAGGAGGAGGAAATGTAGAAAAAGATCCAGATGGTGCTAAAAGCCAAGA
  AAAAGAGGAACCAGAAATCTCCACGGAAAAAGAAGACTCTGCACGTTTGGATGATCACGAAGAGGAGGAG
  GAAGAGGATGAAGAGCCATCCCACAACGAGGACCATGATGCCGATGACGAGGATGACAGCCACATGGAGT
  CTGCCGAAGTGGAGAAGGAAGAGCTGCCCAGAGAAAGCTTCAAAGAAGTACTGGAAAACCAGGAGACTTT
  TTTAGACCTTAATGTGCAGCCTGGTCACTCGAACCCAGAGGTCTTAATGGACTGTGGCGTCGACCTGACA
  GCTTCTTGTAACAGTGAGCCCAAGGAGCTTGCTGGGGACCCTGAAGCTGTACCCGAATCTGACGAGGAGC
  CACCCCCAGGAGAACAGGCACAGAAGCAGGACCAAAAGAACAGCAAGGAAGTCGATACAGAGTTCAAAGA
  GGGAAACCCAGCAACCATGGAAATCGACTCTGAGACTGTCCAGGCCGTTCAGTCTTTGACCCAGGAGAGC
  AGCGAACAGGACGACACCTTTCAGGATTGTGCCGAGACTCAAGAGGCCTGTAGAAGCCTACAGAACTACA
  CCCGTGCAGACCAAAGTCCACAGATTGCCACCACGCTCGACGATTGCCAACAGTCGGACCACAGTAGCCC
  AGTTTCATCCGTCCACTCCCATCCTGGCCAGTCCGTACGTTCTGTCAACAGCCCAAGTGTCCCTGCTCTG
  GAAAACAGCTACGCCCAAATCAGCCCAGATCAAAGTGCCATCTCAGTGCCATCTCTGCAGAACATGGAAA
  CCAGTCCCATGATGGATGTCCCATCAGTTTCAGATCATTCACAGCAAGTCGTAGACAGTGGATTTAGTGA
  CCTGGGCAGTATCGAGAGCACAACTGAGAACTACGAAAACCCAAGCAGCTACGATTCTACTATGGGAGGC
  AGCATCTGTGGAAACGGCTCTTCACAGAACAGCTGCTCCTATAGCAACCTCACCTCCAGCAGTCTGACAC
  AGAGCAGCTGTGCTGTCACCCAGCAGATGTCCAACATCAGCGGGAGCTGCAGCATGCTGCAGCAAACCAG
  CATCAGCTCCCCTCCGACCTGCAGCGTCAAGTCTCCTCAAGGCTGTGTGGTGGAGAGGCCTCCGAGCAGC
  AGCCAGCAGCTGGCTCAGTGCAGCATGGCTGCTAACTTCACCCCACCCATGCAGCTGGCTGAAATCCCCG
  AGACGAGCAACGCCAACATTGGCTTATACGAGCGAATGGGTCAGAGTGATTTTGGGGCTGGGCATTACCC
  GCAGCCGTCAGCCACCTTCAGCCTTGCCAAACTGCAGCAGTTAACTAATACACTTATTGATCATTCATTG
  CCTTACAGCCATTCCGCTGCTGTGACTTCCTATGCAAACAGTGCCTCTTTGTCCACACCATTAAGTAACA
  CAGGGCTTGTTCAACTTTCTCAGTCTCCACACTCCGTCCCTGGGGGACCCCAAGCACAAGCTACCATGAC
  CCCACCCCCCAACCTGACTCCTCCTCCAATGAATCTGCCGCCGCCTCTTTTGCAACGGAACATGGCTGCA
  TCAAATATTGGCATCTCTCACAGCCAAAGACTGCAAACCCAGATTGCCAGCAAGGGCCACATCTCCATGA
  GAACCAAGTCAGCGTCTCTGTCACCAGCCGCTGCCACCCATCAGTCACAAATCTATGGGCGCTCCCAGAC
  TGTAGCCATGCAGGGTCCTGCACGGACTTTAACGATGCAAAGAGGCATGAACATGAGTGTGAACCTGATG
  CCAGCGCCAGCCTACAATGTCAACTCTGTGAACATGAACATGAACACTCTCAACGCCATGAATGGGTACA
  GCATGTCCCAGCCAATGATGAACAGTGGCTACCACAGCAATCATGGCTATATGAATCAAACGCCCCAATA
  CCCTATGCAGATGCAGATGGGCATGATGGGCACCCAGCCATATGCCCAGCAGCCAATGCAGACCCCACCC
  CACGGTAACATGATGTACACGGCCCCCGGACATCACGGCTACATGAACACAGGCATGTCCAAACAGTCTC
  TCAATGGCTCCTACATGAGAAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235335 representing NM_001256469
  Red=Cloning site Green=Tags(s)
  
  MVKLANPLYTEWILEAIQKIKKQKQRPSEERICHAVSTSHGLDKKTVSEQLELSVQDGSVLKVTNKGLAS
  YKDPDNPGRFSSVKPGTFPKSAKGSRGSCNDLRNVDWNKLLRRAIEGLEEPNGSSLKNIEKYLRSQSDLT
  STTNNPAFQQRLRLGAKRAVNNGRLLKDGPQYRVNYGSLDGKGAPQYPSAFPSSLPPVSLLPHEKDQPRA
  DPIPICSFCLGTKESNREKKPEELLSCADCGSSGHPSCLKFCPELTTNVKALRWQCIECKTCSACRVQGR
  NADNMLFCDSCDRGFHMECCDPPLSRMPKGMWICQVCRPKKKGRKLLHEKAAQIKRRYAKPIGRPKNKLK
  QRLLSVTSDEGSMNAFTGRGSPDTEIKINIKQESADVNVIGNKDVVTEEDLDVFKQAQELSWEKIECESG
  VEDCGRYPSVIEFGKYEIQTWYSSPYPQEYARLPKLYLCEFCLKYMKSKNILLRHSKKCGWFHPPANEIY
  RRKDLSVFEVDGNMSKIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVLTKNDEKGCHLVGYFSKEKLCQQK
  YNVSCIMIMPQHQRQGFGRFLIDFSYLLSRREGQAGSPEKPLSDLGRLSYLAYWKSVILEYLYHHHERHI
  SIKAISRATGMCPHDIATTLQHLHMIDKRDGRFVIIRREKLILSHMEKLKTCSRANELDPDSLRWTPILI
  SNAAVSEEEREAEKEAERLMEQASCWEKEEQEILSTRANSRQSPAKVQSKNKYLHSPESRPVTGERGQLL
  ELSKESSEEEEEEEDEEEEEEEEEEEEDEEEEEEEEEEEEEENIQSSPPRLTKPQSVAIKRKRPFVLKKK
  RGRKRRRINSSVTTETISETTEVLNEPFDNSDEERPMPQLEPTCEIEVEEDGRKPVLRKAFQHQPGKKRQ
  TEEEEGKDNHCFKNADPCRNNMNDDSSNLKEGSKDNPEPLKCKQVWPKGTKRGLSKWRQNKERKTGFKLN
  LYTPPETPMEPDEQVTVEEQKETSEGKTSPSPIRIEEEVKETGEALLPQEENRREETCAPVSPNTSPGEK
  PEDDLIKPEEEEEEEEEEEEEEEEEEGEEEEGGGNVEKDPDGAKSQEKEEPEISTEKEDSARLDDHEEEE
  EEDEEPSHNEDHDADDEDDSHMESAEVEKEELPRESFKEVLENQETFLDLNVQPGHSNPEVLMDCGVDLT
  ASCNSEPKELAGDPEAVPESDEEPPPGEQAQKQDQKNSKEVDTEFKEGNPATMEIDSETVQAVQSLTQES
  SEQDDTFQDCAETQEACRSLQNYTRADQSPQIATTLDDCQQSDHSSPVSSVHSHPGQSVRSVNSPSVPAL
  ENSYAQISPDQSAISVPSLQNMETSPMMDVPSVSDHSQQVVDSGFSDLGSIESTTENYENPSSYDSTMGG
  SICGNGSSQNSCSYSNLTSSSLTQSSCAVTQQMSNISGSCSMLQQTSISSPPTCSVKSPQGCVVERPPSS
  SQQLAQCSMAANFTPPMQLAEIPETSNANIGLYERMGQSDFGAGHYPQPSATFSLAKLQQLTNTLIDHSL
  PYSHSAAVTSYANSASLSTPLSNTGLVQLSQSPHSVPGGPQAQATMTPPPNLTPPPMNLPPPLLQRNMAA
  SNIGISHSQRLQTQIASKGHISMRTKSASLSPAAATHQSQIYGRSQTVAMQGPARTLTMQRGMNMSVNLM
  PAPAYNVNSVNMNMNTLNAMNGYSMSQPMMNSGYHSNHGYMNQTPQYPMQMQMGMMGTQPYAQQPMQTPP
  HGNMMYTAPGHHGYMNTGMSKQSLNGSYMRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001256469
• ORF Size: 5343 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256469.1, NP_001243398.1
• RefSeq Size: 7633 bp
• RefSeq ORF: 5346 bp
• Locus ID: 23522
• UniProt ID: Q8WYB5
• Cytogenetics: 10q22.2
• Protein Families: Druggable Genome
• MW: 200.3 kDa
• Gene Summary: The protein encoded by this gene is a histone acetyltransferase and component of the MOZ/MORF protein complex. In addition to its acetyltransferase activity, the encoded protein has transcriptional activation activity in its N-terminal end and transcriptional repression activity in its C-terminal end. This protein is necessary for RUNX2-dependent transcriptional activation and could be involved in brain development. Mutations have been found in patients with genitopatellar syndrome. A translocation of this gene and the CREBBP gene results in acute myeloid leukemias. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]