Myosin Phosphatase (PPP1R12A) (NM_001244992) Human Tagged ORF Clone

CAT#: RC235094

PPP1R12A (Myc-DDK tagged) - Homo sapiens protein phosphatase 1, regulatory subunit 12A (PPP1R12A), transcript variant 5

Product Images

Circular map for RC235094

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myosin Phosphatase
• Synonyms: GUBS; M130; MBS; MYPT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235094 representing NM_001244992
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGGCGGACGCGAAGCAGAAGCGGAACGAGCAGCTGAAACGCTGGATCGGCTCCGAGACGGACC
  TCGAGCCTCCGGTGGTGAAGCGCCAGAAGACCAAGGTGAAGTTCGACGATGGCGCCGTCTTCCTGGCTGC
  TTGCTCCAGCGGCGACACGGACGAGGTCCTCAAGCTGCTGCACCGCGGCGCCGACATCAATTACGCCAAT
  GTGGACGGACTCACTGCCCTGCACCAGGCTTGCATTGATGACAATGTTGATATGGTGAAGTTTCTGGTAG
  AAAATGGAGCAAATATTAATCAACCTGATAATGAAGGCTGGATACCACTACATGCAGCAGCTTCCTGTGG
  ATATCTTGATATTGCAGAGTTTTTGATTGGTCAAGGAGCACATGTAGGGGCTGTCAACAGTGAAGGAGAT
  ACACCTTTAGATATTGCGGAGGAGGAGGCAATGGAAGAGCTACTTCAAAATGAAGTTAATCGGCAAGGGG
  TTGATATAGAAGCAGCTCGAAAGGAAGAAGAACGGATCATGCTTAGAGATGCCAGGCAGTGGCTAAATAG
  TGGTCATATAAATGATGTCCGGCATGCAAAATCTGGAGGTACAGCACTTCACGTTGCAGCTGCTAAAGGC
  TATACGGAAGTTTTAAAACTTTTAATACAGGCAGGCTATGATGTTAATATTAAAGACTATGATGGCTGGA
  CACCTCTTCATGCTGCAGCTCATTGGGGTAAAGAAGAAGCATGTCGAATTTTAGTGGACAATCTGTGTGA
  TATGGAGATGGTCAACAAAGTGGGCCAAACAGCCTTTGATGTAGCAGATGAAGACATTTTAGGATATTTA
  GAAGAGTTGCAAAAGAAACAAAATCTGCTCCATAGTGAAAAACGGGACAAGAAATCTCCACTAATTGAAT
  CAACAGCAAATATGGACAATAATCAGTCACAGAAGACCTTTAAAAACAAAGAGACGTTGATTATTGAACC
  AGAGAAAAATGCATCCCGTATTGAATCTCTGGAACAAGAAAAGGTTGATGAAGAAGAAGAAGGAAAGAAG
  GATGAGTCTAGCTGCTCTAGTGAAGAAGATGAGGAAGATGACTCGGAATCAGAAGCTGAAACAGATAAGA
  CAAAACCCCTGGCTTCTGTAACTAATGCCAACACTTCTAGTACACAAGCAGCTCCTGTAGCTGTTACAAC
  ACCTACTGTGTCATCAGGTCAAGCAACACCTACATCACCTATTAAAAAGTTTCCAACCACAGCTACAAAA
  ATTTCTCCCAAAGAAGAAGAGAGAAAAGATGAGTCTCCTGCAACTTGGAGGTTAGGACTTAGAAAGACGG
  GCAGCTATGGTGCACTTGCTGAAATCACAGCATCTAAAGAGGGTCAGAAAGAAAAAGATACTGCAGGTGT
  TACACGTTCAGCTTCAAGTCCCAGACTTTCCTCCTCTTTGGATAATAAAGAAAAGGAGAAAGATAGTAAA
  GGAACTAGGCTTGCATATGTTGCACCTACAATACCAAGACGACTAGCCAGTACATCTGACATTGAAGAGA
  AAGAAAACAGAGATTCTTCAAGTTTGCGAACAAGTAGTTCATATACAAGGAGAAAATGGGAAGATGATCT
  TAAAAAAAATAGCTCAGTTAATGAAGGATCAACGTATCATAAAAGTACCTCAAATCGTTTGTGGGCTGAG
  GATAGTACTGAGAAAGAAAAGGACAGTGTTCCTACGGCAGTGACCATTCCTGTTGCTCCAACTGTTGTAA
  ATGCTGCAGCTTCTACCACAACCCTGACTACAACTACTGCTGGCACTGTCTCCTCCACAACAGAGGTCAG
  GGAGAGACGCAGATCATACCTCACTCCTGTTAGGGATGAAGAGTCTGAATCCCAAAGAAAAGCAAGATCT
  AGACAAGCAAGACAATCTAGAAGATCAACACAGGGAGTGACATTAACTGATCTTCAAGAAGCTGAGAAAA
  CAATAGGAAGAAGTCGTTCTACCCGAACCAGAGAACAAGAAAATGAAGAAAAAGAAAAAGAGGAAAAAGA
  GAAACAAGATAAAGAGAAACAAGAAGAAAAGAAGGAGTCAGAAACATCTAGAGAAGATGAATATAAACAA
  AAGTACTCCAGAACGTATGATGAGACTTACCAGCGTTATAGGCCAGTATCAACTTCAAGTTCAACCACTC
  CATCCTCTTCACTTTCTACTATGAGCAGTTCACTGTATGCTTCAAGTCAACTAAACAGGCCAAATAGTCT
  TGTAGGCATAACTTCTGCTTACTCCAGAGGAATAACAAAAGAAAATGAAAGAGAGGGAGAAAAAAGAGAA
  GAGGAGAAAGAAGGAGAAGATAAATCACAACCTAAATCAATCAGAGAACGACGACGACCAAGAGAGAAAA
  GAAGATCTACAGGAGTTTCATTTTGGACACAAGATAGTGATGAAAATGAACAAGAACAACAATCAGACAC
  AGAAGAGGGATCCAATAAGAAAGAAACTCAGACGGATTCCATTTCTAGATATGAAACCAGTTCTACATCA
  GCTGGTGATCGATATGATTCCTTGCTGGGTCGCTCTGGATCATACAGTTACTTAGAAGAAAGAAAACCTT
  ACAGCAGCAGGCTAGAAAAGGATGACTCAACTGACTTTAAAAAGCTTTATGAACAAATTCTAGCTGAAAA
  TGAAAAGCTGAAGGCACAGCTACATGATACAAATATGGAACTAACAGATCTTAAATTACAGTTGGAAAAG
  GCCACCCAGAGACAAGAAAGATTTGCTGATAGATCACTGTTGGAAATGGAAAAAAGGGAACGAAGAGCTC
  TAGAAAGAAGAATATCTGAAATGGAAGAAGAGCTCAAAATGTTACCAGACCTAAAAGCAGACAACCAGAG
  GCTAAAGGATGAAAATGGGGCCTTGATCAGAGTTATAAGCAAACTTTCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235094 representing NM_001244992
  Red=Cloning site Green=Tags(s)
  
  MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYAN
  VDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGD
  TPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKG
  YTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYL
  EELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKK
  DESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPTSPIKKFPTTATK
  ISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEGQKEKDTAGVTRSASSPRLSSSLDNKEKEKDSK
  GTRLAYVAPTIPRRLASTSDIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSTSNRLWAE
  DSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYLTPVRDEESESQRKARS
  RQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQ
  KYSRTYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKRE
  EEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTS
  AGDRYDSLLGRSGSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEK
  ATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001244992
• ORF Size: 2922 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001244992.1, NP_001231921.1
• RefSeq Size: 5311 bp
• RefSeq ORF: 2925 bp
• Locus ID: 4659
• UniProt ID: O14974
• Cytogenetics: 12q21.2-q21.31
• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• MW: 110.2 kDa
• Gene Summary: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]