Lipin 1 (LPIN1) (NM_001261427) Human Tagged ORF Clone

CAT#: RC235001

LPIN1 (Myc-DDK tagged) - Homo sapiens lipin 1 (LPIN1), transcript variant 2

Product Images

Circular map for RC235001

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lipin 1
• Synonyms: PAP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235001 representing NM_001261427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGAGTGCAGACCATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAGC
  TCTACAAGGGGCTGAATCCCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAATGG
  AAACCTCCAATGCTCCCCTTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCGAGAGAAAGTG
  GTTGACATAGAAATCAATGGGGAATCTGTGGATTTGCATATGAAATTGGGAGATAATGGAGAAGCATTTT
  TTGTTCAAGAAACAGATAATGATCAGGAAGTTATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGA
  AGGAGCTTCGAGAATGGAATGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGC
  ACGCCAGCCCAAGTGATCGCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAA
  GGAGGAGAAAGTCACAGCTGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAGGATGAGGACAT
  GTTCCCCATCGAGATGAGCTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCTTCCTAATGAT
  ATACCTCCATTCCAAGATGATATTCCTGAGGAAAACCTCTCCCTGGCTGTGATTTACCCTCAGTCAGCCT
  CATACCCTAATTCGGATAGAGAGTGGTCACCCACTCCCAGTCCTTCCGGTTCCCGACCTTCAACACCTAA
  AAGTGATTCAGAATTGGTCAGCAAGTCCACGGAAAGGACAGGGCAGAAGAACCCAGAAATGCTTTGGCTG
  TGGGGAGAGCTGCCGCAGGCTGCTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGCA
  GTAGAAAAATTTGTGATAAAAGTCACTTTCAGGCCATTCACAGCGAATCTTCAGACACTTTTAGTGACCA
  ATCGCCAACTCTGGTCGGTGGGGCACTTTTGGACCAGAACAAGCCTCAGACAGAAATGCAGTTTGTGAAT
  GAAGAAGACCTGGAGACCTTAGGAGCAGCAGCGCCACTCTTGCCCATGATCGAGGAGCTCAAACCCCCCT
  CTGCCAGTGTAGTCCAGACAGCAAACAAGACGGATTCTCCTTCCAGGAAAAGAGATAAACGAAGCCGACA
  TCTTGGTGCTGACGGCGTCTACTTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTT
  CCCAAAAACGGAGATCCTTCCGGACTCGCAAAACATGCAAGCGACAACGGAGCCCGGTCAGCCAACCAGT
  CCCCGCAGTCGGTGGGCAGCTCGGGCGTGGACAGTGGCGTGGAGAGCACCTCGGACGGGCTGAGGGACCT
  CCCTTCCATCGCCATCTCCCTCTGCGGGGGCCTCAGCGACCACCGGGAGATCACGAAAGATGCATTCCTG
  GAGCAAGCTGTGTCATATCAACAGTTTGTGGACAACCCCGCTATTATCGATGACCCCAATCTCGTGGTAA
  AGATTGGGAGTAAATATTATAACTGGACAACAGCAGCACCCCTCCTCCTGGCAATGCAGGCCTTCCAGAA
  ACCTTTGCCAAAGGCCACTGTGGAATCTATCATGAGGGATAAAATGCCCAAAAAGGGAGGAAGATGGTGG
  TTTTCATGGAGGGGAAGAAACACCACAATCAAGGAGGAAAGTAAGCCAGAGCAGTGCTTGGCTGGCAAGG
  CCCATAGCACCGGAGAGCAACCGCCGCAGCTCAGCTTGGCCACCAGGGTAAAGCATGAATCATCCTCCAG
  TGATGAGGAGCGCGCAGCTGCCAAGCCATCAAACGCAGGCCACCTCCCTCTTCTGCCTAATGTCAGCTAC
  AAGAAGACTCTCCGGCTGACTTCCGAGCAGCTTAAAAGCTTGAAGTTGAAGAATGGCCCCAACGACGTGG
  TTTTCAGTGTCACCACGCAGTACCAAGGCACGTGCCGCTGTGAGGGCACCATCTATCTGTGGAACTGGGA
  TGATAAAGTCATCATTTCTGATATTGATGGGACAATTACCAGATCAGATACTCTTGGCCACATTTTGCCC
  ACCCTTGGGAAGGATTGGACCCATCAGGGCATCGCTAAGCTGTACCATAAAGTGAGCCAGAATGGATATA
  AATTTCTCTACTGTTCTGCCCGTGCCATCGGGATGGCGGACATGACGCGGGGCTACCTGCACTGGGTCAA
  CGAGAGGGGCACGGTGCTGCCCCAGGGGCCCCTGCTGCTGAGTCCCAGCAGCCTCTTCTCTGCCCTGCAC
  AGAGAAGTGATTGAAAAGAAGCCAGAAAAGTTTAAAGTCCAGTGTTTGACAGACATCAAAAACCTGTTTT
  TCCCCAACACAGAACCCTTTTATGCTGCTTTTGGAAACCGACCAGCTGATGTGTATTCATACAAGCAAGT
  AGGAGTGTCTTTGAATAGAATATTTACCGTCAACCCTAAAGGAGAGCTGGTACAGGAACATGCAAAGACC
  AACATCTCTTCGTATGTGAGACTCTGTGAAGTAGTCGACCACGTTTTCCCGTTGCTGAAAAGAAGCCATT
  CTTCAGACTTTCCCTGTTCGGATACCTTCAGTAACTTCACCTTTTGGAGAGAGCCACTGCCACCTTTTGA
  AAACCAGGACATTCATTCTGCCTCAGCG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235001 representing NM_001261427
  Red=Cloning site Green=Tags(s)
  
  MSRVQTMNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKV
  VDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPS
  TPAQVIAPSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPND
  IPPFQDDIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWL
  WGELPQAAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVN
  EEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYF
  PKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFL
  EQAVSYQQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWW
  FSWRGRNTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSY
  KKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILP
  TLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALH
  REVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKT
  NISSYVRLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001261427
• ORF Size: 2688 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001261427.2
• RefSeq Size: 5591 bp
• RefSeq ORF: 2691 bp
• Locus ID: 23175
• UniProt ID: Q14693
• Cytogenetics: 2p25.1
• MW: 99.8 kDa
• Gene Summary: This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017]