RUNX1T1 (NM_001198631) Human Tagged ORF Clone

CAT#: RC234543

RUNX1T1 (Myc-DDK tagged) - Homo sapiens runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 12

Product Images

Circular map for RC234543

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234543 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  RCATGATATCTGTCAAAAGAAACACTTGGAGAGCACTGAGTTTAGTAATAGGTGACTGCCGGAAAAAAGG
  GAACTTTGAATATTGTCAAGATCGTACTGAGAAGCACTCCACAATGCCAGACTCACCTGTGGATGTGAAG
  ACGCAATCTAGGCTGACTCCTCCAACAATGCCACCTCCCCCAACTACTCAAGGAGCTCCAAGAACCAGTT
  CATTTACACCGACAACGTTAACTAATGGCACGAGCCATTCTCCTACAGCCTTGAATGGCGCCCCCTCACC
  ACCCAATGGCTTCAGCAATGGGCCTTCCTCTTCTTCCTCCTCCTCTCTGGCTAATCAACAGCTGCCCCCA
  GCCTGTGGTGCCAGGCAACTCAGCAAGCTGAAAAGGTTCCTTACTACCCTGCAGCAGTTTGGCAATGACA
  TTTCACCCGAGATAGGAGAAAGAGTTCGCACCCTCGTTCTGGGACTAGTGAACTCCACTTTGACAATTGA
  AGAATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCCACTGAGACCTTTTGTCATCCCATTTTTGAAG
  GCCAACTTGCCCCTGCTGCAGCGTGAGCTCCTCCACTGCGCAAGACTGGCCAAACAGAACCCTGCCCAGT
  ACCTCGCCCAGCATGAACAGCTGCTTCTGGATGCCAGCACCACCTCACCTGTTGACTCCTCAGAGCTGCT
  TCTCGATGTGAACGAAAACGGGAAGAGGCGAACTCCAGACAGAACCAAAGAAAATGGCTTTGACAGAGAG
  CCTTTGCACTCAGAACATCCAAGCAAGCGACCATGCACTATTAGCCCAGGCCAGCGGTACAGTCCAAATA
  ACGGCTTATCCTACCAGCCCAATGGCCTGCCTCACCCTACCCCACCTCCACCTCAGCATTACCGTTTGGA
  TGATATGGCCATTGCCCACCACTACAGGGACTCCTATCGACACCCCAGCCACAGGGACCTCAGGGACAGA
  AACAGACCTATGGGGTTGCATGGCACACGTCAAGAAGAAATGATTGATCACAGACTAACAGACAGAGAAT
  GGGCAGAAGAGTGGAAACATCTTGACCATCTGTTAAACTGCATAATGGACATGGTAGAAAAAACAAGGCG
  ATCTCTCACCGTACTAAGGCGGTGTCAAGAAGCAGACCGGGAAGAATTGAATTACTGGATCCGGCGGTAC
  AGTGACGCCGAGGACTTAAAAAAAGGTGGCGGCAGTAGCAGCAGCCACTCTAGGCAGCAGAGTCCCGTCA
  ACCCAGACCCAGTTGCACTAGACGCGCATCGGGAATTCCTTCACAGGCCTGCGTCTGGATACGTGCCAGA
  GGAGATCTGGAAGAAAGCTGAGGAGGCCGTCAATGAGGTGAAGCGCCAGGCGATGACGGAGCTGCAGAAG
  GCCGTGTCTGAGGCGGAGCGGAAAGCCCACGACATGATCACAACAGAGAGGGCCAAGATGGAGCGCACGG
  TCGCCGAGGCCAAACGGCAGGCGGCGGAGGACGCACTGGCAGTTATCAATCAGCAGGAGGATTCAAGCGA
  GAGTTGCTGGAATTGTGGCCGTAAAGCGAGTGAAACCTGCAGTGGCTGTAACACAGCCCGATACTGTGGC
  TCATTTTGCCAGCACAAAGACTGGGAGAAGCACCATCACATCTGTGGACAGACCCTGCAGGCCCAGCAGC
  AGGGAGACACACCTGCAGTCAGCTCCTCTGTCACGCCCAACAGCGGGGCTGGGAGCCCGATGGACACACC
  ACCAGCAGCCACTCCGAGGTCAACCACCCCGGGAACCCCTTCCACCATAGAGACAACCCCTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234543 protein sequence
  Red=Cloning site Green=Tags(s)
  
  X*YLSKETLGEH*V***VTAGKKGTLNIVKIVLRSTPQCQTHLWM*RRNLG*LLQQCHLPQLLKELQEPV
  HLHRQR*LMARAILLQP*MAPPHHPMASAMGLPLLPPPLWLINSCPQPVVPGNSAS*KGSLLPCSSLAMT
  FHPR*EKEFAPSFWD**TPL*QLKNFIPNCKKLLTSH*DLLSSHF*RPTCPCCSVSSSTAQDWPNRTLPS
  TSPSMNSCFWMPAPPHLLTPQSCFSM*TKTGRGELQTEPKKMALTESLCTQNIQASDHALLAQASGTVQI
  TAYPTSPMACLTLPHLHLSITVWMIWPLPTTTGTPIDTPATGTSGTETDLWGCMAHVKKK*LITD*QTEN
  GQKSGNILTIC*TA*WTW*KKQGDLSPY*GGVKKQTGKN*ITGSGGTVTPRT*KKVAAVAAATLGSRVPS
  TQTQLH*TRIGNSFTGLRLDTCQRRSGRKLRRPSMR*SARR*RSCRRPCLRRSGKPTT*SQQRGPRWSAR
  SPRPNGRRRRTHWQLSISRRIQARVAGIVAVKRVKPAVAVTQPDTVAHFASTKTGRSTITSVDRPCRPSS
  RETHLQSAPLSRPTAGLGARWTHHQQPLRGQPPREPLPP*RQPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198631
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198631.1, NP_001185560.1
• RefSeq Size: 7483 bp
• RefSeq ORF: 1815 bp
• Locus ID: 862
• UniProt ID: Q06455
• Cytogenetics: 8q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• MW: 67.6 kDa
• Gene Summary: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]