CREB3L3 (NM_001271995) Human Tagged ORF Clone

CAT#: RC234165

CREB3L3 (Myc-DDK tagged) - Homo sapiens cAMP responsive element binding protein 3-like 3 (CREB3L3), transcript variant 2

Product Images

Circular map for RC234165

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CREB3L3
• Synonyms: CREB-H; CREBH; HYST1481; HYTG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234165 representing NM_001271995
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACGGATTTAGCTGCTGGAAAGATGGCTTCTGCTGCCTGCTCCATGGACCCCATCGACAGCTTTG
  AGCTCCTGGATCTCCTGTTTGACCGGCAGGACGGCATCCTGAGACACGTGGAGCTGGGCGAGGGCTGGGG
  TCACGTCAAGGACCAGGTCCTGCCAAACCCCGACTCTGACGACTTCCTCAGCTCCATCCTGGGCTCTGGA
  GACTCACTGCCCAGCTCCCCACTCTGGTCCCCCGAAGGCAGTGATAGTGGCATCTCCGAAGACCTCCCCT
  CCGACCCCCAGGACACCCCTCCACGCAGCGGACCAGCCACCTCCCCCGCCGGCTGCCATCCTGCCCAGCC
  TGGCAAGGGGCCCTGCCTCTCCTATCATCCTGGCAACTCTTGCTCCACCACAACCCCAGGGCCAGTGATC
  CAAGTACCTGAAGCCTCTGTGACCATAGACCTGGAAATGTGGAGCCCAGGAGGAAGGATCTGTGCTGAGA
  AGCCGGCTGATCCGGTGGACCTGTCCCCACGATGCAATCTCACCGTGAAAGACCTCCTCCTTTCGGGCAG
  CAGTGGGGACCTGCAACAGCATCACCTGGGGGCCTCCTACCTCCTGCGACCTGGGGCTGGGCACTGTCAG
  GAGCTGGTGCTCACCGAGGATGAGAAGAAGCTGCTGGCTAAAGAAGGCATCACCCTGCCCACTCAGCTGC
  CCCTCACTAAGTACGAGGAGCGAGTGCTGAAAAAAATCCGCCGGAAAATCCGGAACAAGCAGTCGGCGCA
  AGAAAGCAGGAAGAAGAAGAAGGAATATATCGATGGCCTGGAGACTCGGATGTCAGCTTGCACTGCTCAG
  AATCAGGAGTTACAGAGGAAAGTCTTGCATCTCGAGAAGCAAAACCTGTCCCTCTTGGAGCAACTGAAGA
  AACTCCAGGCCATTGTGGTGCAGTCCACCAGCAAGTCAGCCCAGACAGGCACCTGTGTCGCAGTCCTGTT
  GCTGTCCTTTGCCCTCATCATCCTCCCCTCCATCAGCCCTTTTGGCCCCAACAAAACCGAGAGCCCTGGG
  GACTTTGCGCCTGTACGAGTGTTCTCCAGAACTTTGCACAACGATGCTGCCTCCCGCGTGGCTGCTGATG
  CTGTGCCAGGCTCCGAGGCCCCAGGACCCCGACCCGAGGCTGACACAACCCGAGAAGAGTCTCCAGGAAG
  CCCCGGGGCAGACTGGGGCTTCCAGGACACCGCGAACCTGACCAATTCGACGGAGGAGCTGGACAACGCC
  ACCCTGGTCCTGAGGAATGCAACAGAGGGGCTGGGCCAGGTCGCCCTGCTGGACTGGGTGGCGCCTGGGC
  CGAGCACTGGCTCAGGACGTGCAGGGCTGGAGGCGGCGGGAGACGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234165 representing NM_001271995
  Red=Cloning site Green=Tags(s)
  
  MNTDLAAGKMASAACSMDPIDSFELLDLLFDRQDGILRHVELGEGWGHVKDQVLPNPDSDDFLSSILGSG
  DSLPSSPLWSPEGSDSGISEDLPSDPQDTPPRSGPATSPAGCHPAQPGKGPCLSYHPGNSCSTTTPGPVI
  QVPEASVTIDLEMWSPGGRICAEKPADPVDLSPRCNLTVKDLLLSGSSGDLQQHHLGASYLLRPGAGHCQ
  ELVLTEDEKKLLAKEGITLPTQLPLTKYEERVLKKIRRKIRNKQSAQESRKKKKEYIDGLETRMSACTAQ
  NQELQRKVLHLEKQNLSLLEQLKKLQAIVVQSTSKSAQTGTCVAVLLLSFALIILPSISPFGPNKTESPG
  DFAPVRVFSRTLHNDAASRVAADAVPGSEAPGPRPEADTTREESPGSPGADWGFQDTANLTNSTEELDNA
  TLVLRNATEGLGQVALLDWVAPGPSTGSGRAGLEAAGDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271995
• ORF Size: 1380 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271995.2
• RefSeq Size: 2621 bp
• RefSeq ORF: 1383 bp
• Locus ID: 84699
• UniProt ID: Q68CJ9
• Cytogenetics: 19p13.3
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Melanogenesis, Prostate cancer
• MW: 49.4 kDa
• Gene Summary: This gene encodes a member of the basic-leucine zipper family and the AMP-dependent transcription factor family. The encoded protein is localized to the endoplasmic reticulum and acts as a transcription factor activated by cyclic AMP stimulation. The encoded protein binds the cyclic AMP response element (CRE) and the box-B element and has been linked to acute inflammatory response, hepatocellular carcinoma, triglyceride metabolism, and hepcidin expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]