PDE1C (NM_001191056) Human Tagged ORF Clone

SKU RC233033

PDE1C (Myc-DDK tagged) - Homo sapiens phosphodiesterase 1C, calmodulin-dependent 70kDa (PDE1C), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: PDE1C
• Synonyms: cam-PDE 1C; DFNA74; hCam-3; Hcam3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC233033 representing NM_001191056; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGTCGCCAACCAAGGAGATTGAAGAATTTGAGAGCAACTCTCTGAAATACCTGCAACCGGAACAGA; TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGAGGAAATATAAGAAAACGTCCCAGAGATTACGGTCTTT; GGTCAAACAATTAGAGAGAGGGGAAGCTTCAGTGGTAGATCTTAAGAAGAATTTGGAATATGCAGCCACA; GTGCTTGAATCTGTGTATATTGATGAAACAAGGAGACTCCTGGATACAGAGGATGAGCTCAGTGACATTC; AGTCAGATGCTGTGCCTTCTGAGGTCCGAGACTGGCTGGCCTCCACCTTCACGCGGCAGATGGGGATGAT; GCTCAGGAGGAGCGACGAGAAGCCCCGGTTCAAGAGCATCGTTCACGCAGTGCAGGCTGGGATATTTGTG; GAGAGAATGTATAGACGGACATCAAACATGGTTGGACTGAGCTATCCACCAGCTGTTATTGAGGCATTAA; AGGATGTGGACAAGTGGTCCTTTGACGTCTTTTCCCTCAATGAGGCCAGTGGGGATCATGCACTGAAATT; TATTTTCTATGAACTACTCACACGTTATGATCTGATCAGCCGTTTCAAGATCCCCATTTCTGCACTTGTC; TCATTTGTGGAGGCCCTGGAAGTGGGATACAGCAAGCACAAAAATCCTTACCATAACTTAATGCACGCTG; CCGATGTTACACAGACAGTGCATTACCTCCTCTATAAGACAGGAGTGGCGAACTGGCTGACGGAGCTGGA; GATCTTTGCTATAATCTTCTCAGCTGCCATCCATGACTACGAGCATACCGGAACCACCAACAATTTCCAC; ATTCAGACTCGGTCTGATCCAGCTATTCTGTATAATGACAGATCTGTACTGGAGAATCACCATTTAAGTG; CAGCTTATCGCCTTCTGCAAGATGACGAGGAAATGAATATTTTGATTAACCTCTCAAAGGATGACTGGAG; GGAGTTTCGAACCTTGGTAATTGAAATGGTGATGGCCACAGATATGTCTTGTCACTTCCAACAAATCAAA; GCAATGAAGACTGCTCTGCAGCAGCCAGAAGCCATTGAAAAGCCAAAAGCCTTATCCCTTATGCTGCATA; CAGCAGATATTAGCCATCCAGCAAAAGCATGGGACCTCCATCATCGCTGGACAATGTCACTCCTGGAGGA; GTTCTTCAGACAGGGTGACAGAGAAGCAGAGCTGGGGCTGCCTTTTTCTCCTCTGTGTGACCGAAAGTCC; ACTATGGTTGCTCAGTCACAAGTAGGTTTCATTGATTTCATCGTGGAACCCACCTTCACTGTGCTTACGG; ACATGACCGAGAAGATTGTGAGTCCATTAATCGATGAAACCTCTCAAACTGGTGGGACAGGACAGAGGCG; TTCGAGTTTGAATAGCATCAGCTCGTCAGATGCCAAGCGATCAGGTGTCAAGACCTCTGGTTCAGAGGGA; AGTGCCCCGATCAACAATTCTGTCATCTCCGTTGACTATAAGAGCTTTAAAGCTACTTGGACGGAAGTGG; TGCACATCAATCGGGAGAGATGGAGGGCCAAGGTACCCAAAGAGGAGAAGGCCAAGAAGGAAGCAGAGGA; AAAGGCTCGCCTGGCCGCAGAGGAGCAGCAAAAGGAAATGGAAGCCAAAAGCCAGGCTGAAGAAGGCGCA; TCTGGCAAAGCTGAGAAAAAGACGTCTGGAGAAACTAAGAATCAAGTCAATGGAACACGGGCAAACAAAA; GTGACAACCCTCGTGGGAAAAACTCCAAAGCCGAGAAGTCATCAGGAGAACAGCAACAGAATGGTGACTT; CAAAGATGGTAAAAATAAGACAGACAAGAAGGATCACTCTAACATCGGAAATGATTCAAAGAAAACAGAT; GATTCACAAGAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC233033 representing NM_001191056; Red=Cloning site Green=Tags(s); ; MESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQLERGEASVVDLKKNLEYAAT; VLESVYIDETRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFV; ERMYRRTSNMVGLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALV; SFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFH; IQTRSDPAILYNDRSVLENHHLSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIK; AMKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKS; TMVAQSQVGFIDFIVEPTFTVLTDMTEKIVSPLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEG; SAPINNSVISVDYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEMEAKSQAEEGA; SGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQQQNGDFKDGKNKTDKKDHSNIGNDSKKTD; DSQE; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191056
• ORF Size: 1902 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001191056.3
• RefSeq Size: 2979 bp
• RefSeq ORF: 1905 bp
• Locus ID: 5137
• UniProt ID: Q14123
• Cytogenetics: 7p14.3
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Olfactory transduction, Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 72.7 kDa
• Summary: This gene encodes an enzyme that belongs to the 3'5'-cyclic nucleotide phosphodiesterase family. Members of this family catalyze hydrolysis of the cyclic nucleotides, cyclic adenosine monophosphate and cyclic guanosine monophosphate, to the corresponding nucleoside 5'-monophosphates. The enzyme encoded by this gene regulates proliferation and migration of vascular smooth muscle cells, and neointimal hyperplasia. This enzyme also plays a role in pathological vascular remodeling by regulating the stability of growth factor receptors, such as PDGF-receptor-beta. [provided by RefSeq, Jul 2016]