KCTD1 (NM_001258222) Human Tagged ORF Clone

CAT#: RC232328

KCTD1 (Myc-DDK tagged) - Homo sapiens potassium channel tetramerization domain containing 1 (KCTD1), transcript variant 5

Product Images

Circular map for RC232328

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD1
• Synonyms: C18orf5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC232328 representing NM_001258222
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCAGGACAGTCGGCCCAATATGTCAAGACCTCTGATCACTAGATCCCCTGCATCTCCACTGAACA
  ACCAAGGCATCCCTACTCCAGCACAACTCACAAAATCCAATGCGCCTGTCCACATTGATGTGGGCGGCCA
  CATGTACACCAGCAGCCTGGCCACCCTCACCAAATACCCTGAATCCAGAATCGGAAGACTTTTTGATGGT
  ACAGAGCCCATTGTTTTGGACAGTCTCAAACAGCACTATTTCATTGACAGAGATGGACAGATGTTCAGAT
  ATATCTTGAATTTTCTACGAACATCCAAACTCCTCATTCCTGATGATTTCAAGGACTACACTTTGTTATA
  TGAAGAGGCAAAATATTTTCAGCTTCAGCCCATGTTGTTGGAGATGGAAAGATGGAAGCAGGACAGAGAA
  ACTGGTCGATTTTCAAGGCCCTGTGAGTGCCTCGTCGTGCGTGTGGCCCCAGACCTCGGAGAAAGGATCA
  CGCTAAGCGGTGACAAATCCTTGATAGAAGAAGTATTTCCAGAGATCGGCGACGTGATGTGTAACTCTGT
  CAATGCAGGCTGGAATCACGACTCGACGCACGTCATCAGGTTTCCACTAAATGGCTACTGTCACCTCAAC
  TCAGTCCAGGTCCTCGAGAGGTTGCAGCAAAGAGGATTTGAAATCGTGGGCTCCTGTGGGGGAGGAGTAG
  ACTCGTCCCAGTTCAGCGAATACGTCCTTCGGCGGGAACTGAGGCGGACGCCCCGTGTACCCTCCGTCAT
  CCGGATAAAGCAAGAGCCTCTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC232328 representing NM_001258222
  Red=Cloning site Green=Tags(s)
  
  MFQDSRPNMSRPLITRSPASPLNNQGIPTPAQLTKSNAPVHIDVGGHMYTSSLATLTKYPESRIGRLFDG
  TEPIVLDSLKQHYFIDRDGQMFRYILNFLRTSKLLIPDDFKDYTLLYEEAKYFQLQPMLLEMERWKQDRE
  TGRFSRPCECLVVRVAPDLGERITLSGDKSLIEEVFPEIGDVMCNSVNAGWNHDSTHVIRFPLNGYCHLN
  SVQVLERLQQRGFEIVGSCGGGVDSSQFSEYVLRRELRRTPRVPSVIRIKQEPLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001258222
• ORF Size: 795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258222.3
• RefSeq Size: 1671 bp
• RefSeq ORF: 798 bp
• Locus ID: 284252
• Cytogenetics: 18q11.2
• Protein Families: Ion Channels: Other
• MW: 30.8 kDa
• Gene Summary: This gene encodes a protein containing a BTB (Broad-complex, tramtrack and bric a brac), also known as a POZ (POxvirus and zinc finger) protein-protein interaction domain. The encoded protein negatively regulates the AP-2 family of transcription factors and the Wnt signaling pathway. A mechanism for the modulation of Wnt signaling has been proposed in which the encoded protein enhances ubiquitination and degradation of the beta-catenin protein. Mutations in this gene have been identified in Scalp-ear-nipple (SEN) syndrome. [provided by RefSeq, May 2017]