NT5C1B (NM_001199087) Human Tagged ORF Clone

CAT#: RC231342

NT5C1B (Myc-DDK-tagged)-Human 5'-nucleotidase, cytosolic IB (NT5C1B), transcript variant 4

Product Images

Circular map for RC231342

• 

  Western blot validation of overexpression lysate (Cat# LY434341) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231342 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NT5C1B
• Synonyms: AIRP; CN-IB; CN1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231342 representing NM_001199087
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTCAAACATCTCTCAAACAGAAAAAGAATGAGCCTGGAATGAGGTCCTCAAAAGAGAGTCTAGAAG
  CAGAAAAAAGAAAGGAATCTGACAAAACAGGAGTTCGTCTGAGCAATCAGATGAGGCGTGCAGTCAATCC
  GAATCACTCGCTGAGATGTTGCCCCTTCCAGGGTCACTCGTCGTGTAGACGCTGCCTTTGTGCAGCTGAG
  GGAACAGCCCTTGGCCCCTGCCACACAATACGTATTTATATTCACATGTGCCTGTTGTGGGAGCAGGGCC
  AGCAGATCACCATGATGAGGGTGAGCAGAGATCACACTACCGATTCTGGAGGGGAACCACAGACAACCTG
  GGGATCACAAGAATCATCACTGCGGAAGACAGACTCTCGAGGGTACCTTGTGCGCAGTCAATGGTCTAGA
  ATATCCCGGAGCCCATCCACCAAGGCTCCATCCATAGATGAGCCTAGAAGCAGGAACACCAGTGCTAAGC
  TCCCCAGCAGCTCCACGAGCTCCCGGACTCCATCCACCTCCCCAAGCCTGCATGACTCCTCACCGCCGCC
  GCTGTCCGGGCAGCCCTCGCTCCAGCCACCCGCGTCGCCCCAGCTGCCCCGGTCGCTGGACTCGCGGCCT
  CCCACGCCCCCAGAGCCCGATCCTGGCTCCCGGCGCAGCACCAAAATGCAAGAGAATCCGGAGGCCTGGG
  CCCAAGGCATCGTGCGGGAAATCCGCCAGACCCGGGACTCGCAGCCGCTGGAATATTCGCGCACGTCCCC
  CACCGAGTGGAAGTCCTCCAGCCAGCGCAGGGGGATCTACCCCGCCTCCACCCAGCTGGACCGCAACTCT
  CTGTCCGAGCAGCAGCAGCAGCAGCGGGAGGACGAGGACGACTACGAGGCTGCCTACTGGGCATCCATGA
  GGTCGTTCTACGAGAAGAACCCGAGCTGCTCGCGCCCCTGGCCGCCCAAACCCAAGAACGCCATCACCAT
  TGCTCTCTCATCCTGCGCGCTCTTCAACATGGTGGACGGCAGGAAAATCTACGAGCAAGAGGGTCTGGAA
  AAGTACATGGAGTATCAGCTCACCAATGAGAACGTCATCCTGACCCCGGGCCCGGCGTTCCGCTTCGTCA
  AGGCACTACAGTATGTCAATGCTAGACTCCGTGATCTATATCCTGATGAACAGGACTTATTTGATATTGT
  ACTGATGACTAATAACCATGCCCAAGTGGGAGTGCGGCTTATAAACAGCGTCAATCACTACGGCTTACTG
  ATTGACCGCTTCTGTCTGACCGGGGGAAAAGACCCCATTGGCTATTTGAAGGCATATCTTACCAACTTGT
  ATATTGCTGCAGATTCTGAAAAAGTGCAAGAGGCAATACAAGAAGGTATTGCCTCTGCGACAATGTTTGA
  TGGAGCCAAAGACATGGCTTACTGTGACACTCAGCTCCGTGTAGCCTTTGATGGGGATGCTGTCCTCTTC
  TCTGATGAGTCTGAACATTTTACCAAGGAGCATGGGCTCGACAAATTCTTCCAGTATGATACATTATGTG
  AAAGTAAGCCTCTTGCTCAGGGTCCCCTAAAAGGCTTTCTGGAAGATTTAGGCAGACTGCAAAAGAAGTT
  CTATGCCAAAAATGAACGGTTACTTTGTCCTATCAGGACCTACCTGGTTACAGCTAGGAGTGCAGCCAGT
  TCAGGCGCCCGTGTGCTGAAGACCCTTCGACGCTGGGGTCTAGAGATAGACGAAGCTCTTTTCCTTGCTG
  GAGCCCCCAAAAGTCCCATCTTGGTGAAGATCCGGCCCCACATCTTCTTTGATGACCACATGTTCCACAT
  TGAAGGGGCACAGAGGTTAGGTTCCATCGCAGCTTATGGCTTTAATAAAAAATTCAGTAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231342 representing NM_001199087
  Red=Cloning site Green=Tags(s)
  
  MSQTSLKQKKNEPGMRSSKESLEAEKRKESDKTGVRLSNQMRRAVNPNHSLRCCPFQGHSSCRRCLCAAE
  GTALGPCHTIRIYIHMCLLWEQGQQITMMRVSRDHTTDSGGEPQTTWGSQESSLRKTDSRGYLVRSQWSR
  ISRSPSTKAPSIDEPRSRNTSAKLPSSSTSSRTPSTSPSLHDSSPPPLSGQPSLQPPASPQLPRSLDSRP
  PTPPEPDPGSRRSTKMQENPEAWAQGIVREIRQTRDSQPLEYSRTSPTEWKSSSQRRGIYPASTQLDRNS
  LSEQQQQQREDEDDYEAAYWASMRSFYEKNPSCSRPWPPKPKNAITIALSSCALFNMVDGRKIYEQEGLE
  KYMEYQLTNENVILTPGPAFRFVKALQYVNARLRDLYPDEQDLFDIVLMTNNHAQVGVRLINSVNHYGLL
  IDRFCLTGGKDPIGYLKAYLTNLYIAADSEKVQEAIQEGIASATMFDGAKDMAYCDTQLRVAFDGDAVLF
  SDESEHFTKEHGLDKFFQYDTLCESKPLAQGPLKGFLEDLGRLQKKFYAKNERLLCPIRTYLVTARSAAS
  SGARVLKTLRRWGLEIDEALFLAGAPKSPILVKIRPHIFFDDHMFHIEGAQRLGSIAAYGFNKKFSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199087
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199087.2
• RefSeq ORF: 1884 bp
• Locus ID: 93034
• UniProt ID: Q96P26
• Cytogenetics: 2p24.2
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism, Purine metabolism, Pyrimidine metabolism
• MW: 71.1 kDa
• Gene Summary: Cytosolic 5-prime nucleotidases, such as NT5C1B, catalyze production of adenosine, which regulates diverse physiologic processes (Sala-Newby and Newby, 2001 [PubMed 11690631]).[supplied by OMIM, Mar 2008]