RUNX1T1 (NM_001198634) Human Tagged ORF Clone

CAT#: RC231339

RUNX1T1 (Myc-DDK-tagged)-Human runt-related transcription factor 1, translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 14

Product Images

Circular map for RC231339

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231339 representing NM_001198634
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCCATCCTGACAAGGCTTTTACTTCTGATAAGCTCCAATGTGTGTTTAATGAATACAAAGCCGCGG
  TCTGGGTGCCGCCTCGGCCGCGGCCGCTCTCCCGCGCTCCTTTGCCAGAAGATCGTACTGAGAAGCACTC
  CACAATGCCAGACTCACCTGTGGATGTGAAGACGCAATCTAGGCTGACTCCTCCAACAATGCCACCTCCC
  CCAACTACTCAAGGAGCTCCAAGAACCAGTTCATTTACACCGACAACGTTAACTAATGGCACGAGCCATT
  CTCCTACAGCCTTGAATGGCGCCCCCTCACCACCCAATGGCTTCAGCAATGGGCCTTCCTCTTCTTCCTC
  CTCCTCTCTGGCTAATCAACAGCTGCCCCCAGCCTGTGGTGCCAGGCAACTCAGCAAGCTGAAAAGGTTC
  CTTACTACCCTGCAGCAGTTTGGCAATGACATTTCACCCGAGATAGGAGAAAGAGTTCGCACCCTCGTTC
  TGGGACTAGTGAACTCCACTTTGACAATTGAAGAATTTCATTCCAAACTGCAAGAAGCTACTAACTTCCC
  ACTGAGACCTTTTGTCATCCCATTTTTGAAGGCCAACTTGCCCCTGCTGCAGCGTGAGCTCCTCCACTGC
  GCAAGACTGGCCAAACAGAACCCTGCCCAGTACCTCGCCCAGCATGAACAGCTGCTTCTGGATGCCAGCA
  CCACCTCACCTGTTGACTCCTCAGAGCTGCTTCTCGATGTGAACGAAAACGGGAAGAGGCGAACTCCAGA
  CAGAACCAAAGAAAATGGCTTTGACAGAGAGCCTTTGCACTCAGAACATCCAAGCAAGCGACCATGCACT
  ATTAGCCCAGGCCAGCGGTACAGTCCAAATAACGGCTTATCCTACCAGCCCAATGGCCTGCCTCACCCTA
  CCCCACCTCCACCTCAGCATTACCGTTTGGATGATATGGCCATTGCCCACCACTACAGGGACTCCTATCG
  ACACCCCAGCCACAGGGACCTCAGGGACAGAAACAGACCTATGGGGTTGCATGGCACACGTCAAGAAGAA
  ATGATTGATCACAGACTAACAGACAGAGAATGGGCAGAAGAGTGGAAACATCTTGACCATCTGTTAAACT
  GCATAATGGACATGGTAGAAAAAACAAGGCGATCTCTCACCGTACTAAGGCGGTGTCAAGAAGCAGACCG
  GGAAGAATTGAATTACTGGATCCGGCGGTACAGTGACGCCGAGGACTTAAAAAAAGGTGGCGGCAGTAGC
  AGCAGCCACTCTAGGCAGCAGAGTCCCGTCAACCCAGACCCAGTTGCACTAGACGCGCATCGGGAATTCC
  TTCACAGGCCTGCGTCTGGATACGTGCCAGAGGAGATCTGGAAGAAAGCTGAGGAGGCCGTCAATGAGGT
  GAAGCGCCAGGCGATGACGGAGCTGCAGAAGGCCGTGTCTGAGGCGGAGCGGAAAGCCCACGACATGATC
  ACAACAGAGAGGGCCAAGATGGAGCGCACGGTCGCCGAGGCCAAACGGCAGGCGGCGGAGGACGCACTGG
  CAGTTATCAATCAGCAGGAGGATTCAAGCGAGAGTTGCTGGAATTGTGGCCGTAAAGCGAGTGAAACCTG
  CAGTGGCTGTAACACAGCCCGATACTGTGGCTCATTTTGCCAGCACAAAGACTGGGAGAAGCACCATCAC
  ATCTGTGGACAGACCCTGCAGGCCCAGCAGCAGGGAGACACACCTGCAGTCAGCTCCTCTGTCACGCCCA
  ACAGCGGGGCTGGGAGCCCGATGGACACACCACCAGCAGCCACTCCGAGGTCAACCACCCCGGGAACCCC
  TTCCACCATAGAGACAACCCCTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231339 representing NM_001198634
  Red=Cloning site Green=Tags(s)
  
  MCHPDKAFTSDKLQCVFNEYKAAVWVPPRPRPLSRAPLPEDRTEKHSTMPDSPVDVKTQSRLTPPTMPPP
  PTTQGAPRTSSFTPTTLTNGTSHSPTALNGAPSPPNGFSNGPSSSSSSSLANQQLPPACGARQLSKLKRF
  LTTLQQFGNDISPEIGERVRTLVLGLVNSTLTIEEFHSKLQEATNFPLRPFVIPFLKANLPLLQRELLHC
  ARLAKQNPAQYLAQHEQLLLDASTTSPVDSSELLLDVNENGKRRTPDRTKENGFDREPLHSEHPSKRPCT
  ISPGQRYSPNNGLSYQPNGLPHPTPPPPQHYRLDDMAIAHHYRDSYRHPSHRDLRDRNRPMGLHGTRQEE
  MIDHRLTDREWAEEWKHLDHLLNCIMDMVEKTRRSLTVLRRCQEADREELNYWIRRYSDAEDLKKGGGSS
  SSHSRQQSPVNPDPVALDAHREFLHRPASGYVPEEIWKKAEEAVNEVKRQAMTELQKAVSEAERKAHDMI
  TTERAKMERTVAEAKRQAAEDALAVINQQEDSSESCWNCGRKASETCSGCNTARYCGSFCQHKDWEKHHH
  ICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTPPAATPRSTTPGTPSTIETTPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001198634
• ORF Size: 1845 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001198634.2
• RefSeq ORF: 1848 bp
• Locus ID: 862
• UniProt ID: Q06455
• Cytogenetics: 8q21.3
• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer
• MW: 69.2 kDa
• Gene Summary: This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]