SCHIP1 (IQCJ-SCHIP1) (NM_001197114) Human Tagged ORF Clone

CAT#: RC231305

IQCJ (Myc-DDK-tagged)-Human IQCJ-SCHIP1 readthrough (IQCJ-SCHIP1), transcript variant 2

Product Images

Circular map for RC231305

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  Western blot validation of overexpression lysate (Cat# LY434304) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231305 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IQCJ-SCHIP1 protein (Cat# TP331305). The protein was produced from HEK293T cells transfected with IQCJ-SCHIP1 cDNA clone (Cat# RC231305) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCHIP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231305 representing NM_001197114
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTCTGGAAGAACTGAAAAGATTGCAGAATCCTCTAGAACAAGTTAATGATGGAAAATATTCATTTG
  AAAACATTCAGCGAGCATGGCGAGAGTACCTGCAGCGGCAGGAGCCCCTGGGGAAGAGGAGCCCGTCCCC
  ACCCTCTGTCTCCTCAGAGAAGCTGAGCAGCTCTGTCAGCATGAACACCTTCTCCGACAGCAGCACACCC
  GATTACCGAGAGGATGGGATGGATCTAGGCAGTGACGCCGGCAGCAGCAGCAGCAGCAGCCGCGCCAGTT
  CACAGTCCAACTCCACCAAAGTGACCCCTTGCTCCGAGTGCAAATCTTCATCGTCGCCGGGGGGCAGCCT
  GGACTTGGTGTCTGCCCTGGAGGACTATGAGGAGCCCTTCCCGGTCTACCAGAAGAAGGTGATTGATGAG
  TGGGCGCCGGAGGAGGACGGGGAGGAGGAGGAAGAGGAGGACGAGCGCGACCAGCGAGGGTACCGGGATG
  ACCGCTCTCCGGCCCGGGAACCGGGGGACGTAAGCGCCAGGACCCGCAGCGGCGGCGGCGGGGGCAGGAG
  CGCCACCACCGCCATGCCGCCCCCGGTGCCCAACGGCAACCTCCACCAGCACGACCCCCAGGACCTCAGG
  CACAATGGCAACGTGGTGGTGGCTGGCCGGCCGAGCTGTTCCCGGGGCCCCCGCCGGGCGATCCAAAAGC
  CCCAGCCGGCTGGGGGCCGGCGCAGTGGCCGCGGCCCGGCGGCTGGGGGGCTCTGCCTTCAGCCCCCAGA
  CGGCGGGACGTGCGTCCCCGAAGAGCCCCCGGTGCCACCTATGGATTGGGAGGCGCTGGAGAAGCATCTG
  GCCGGGCTGCAGTTCCGGGAGCAGGAGGTACGGAACCAGGGCCAGGCGAGGACCAACTCCACCTCCGCAC
  AGAAAAATGAGAGAGAGTCTATCAGACAGAAGTTGGCACTTGGAAGCTTCTTTGATGATGGCCCAGGAAT
  TTATACCAGCTGTAGCAAAAGTGGGAAGCCAAGCCTTTCCTCCCGACTGCAGAGTGGGATGAACTTGCAG
  ATATGCTTTGTCAACGACAGTGGCAGTGATAAGGACAGTGATGCTGATGACAGTAAGACTGAAACCAGCT
  TGGACACCCCCTTGTCTCCCATGAGCAAACAGAGTTCTTCCTATTCTGATAGAGACACTACTGAAGAGGA
  GTCTGAATCCTTGGATGACATGGACTTCCTTACAAGGCAAAAGAAATTGCAAGCTGAAGCCAAAATGGCC
  CTTGCCATGGCCAAACCAATGGCCAAAATGCAAGTAGAAGTGGAGAAACAGAACAGGAAAAAGTCTCCCG
  TCGCTGATCTTCTGCCACACATGCCTCATATAAGTGAATGCTTGATGAAAAGAAGTTTAAAACCCACCGA
  CCTGAGAGACATGACTATTGGGCAGCTACAAGTGATAGTCAATGATCTCCATTCCCAGATAGAAAGCTTG
  AATGAAGAGTTGGTCCAGCTGCTTCTCATCCGAGATGAGCTGCACACAGAGCAGGATGCCATGCTGGTGG
  ACATTGAAGACTTGACCAGACATGCTGAAAGTCAGCAGAAGCACATGGCAGAGAAAATGCCTGCAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231305 representing NM_001197114
  Red=Cloning site Green=Tags(s)
  
  MRLEELKRLQNPLEQVNDGKYSFENIQRAWREYLQRQEPLGKRSPSPPSVSSEKLSSSVSMNTFSDSSTP
  DYREDGMDLGSDAGSSSSSSRASSQSNSTKVTPCSECKSSSSPGGSLDLVSALEDYEEPFPVYQKKVIDE
  WAPEEDGEEEEEEDERDQRGYRDDRSPAREPGDVSARTRSGGGGGRSATTAMPPPVPNGNLHQHDPQDLR
  HNGNVVVAGRPSCSRGPRRAIQKPQPAGGRRSGRGPAAGGLCLQPPDGGTCVPEEPPVPPMDWEALEKHL
  AGLQFREQEVRNQGQARTNSTSAQKNERESIRQKLALGSFFDDGPGIYTSCSKSGKPSLSSRLQSGMNLQ
  ICFVNDSGSDKDSDADDSKTETSLDTPLSPMSKQSSSYSDRDTTEEESESLDDMDFLTRQKKLQAEAKMA
  LAMAKPMAKMQVEVEKQNRKKSPVADLLPHMPHISECLMKRSLKPTDLRDMTIGQLQVIVNDLHSQIESL
  NEELVQLLLIRDELHTEQDAMLVDIEDLTRHAESQQKHMAEKMPAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001197114
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001197114.1, NP_001184043.1
• RefSeq ORF: 1611 bp
• Locus ID: 100505385
• UniProt ID: Q9P0W5
• Cytogenetics: 3q25.32-q25.33
• MW: 59.6 kDa
• Gene Summary: This locus represents naturally occurring read-through transcription from the neighboring IQ motif containing J (IQCJ) and schwannomin interacting protein 1 (SCHIP1) genes. Alternative splicing results in multiple transcript variants that are composed of in-frame exons from each individual gene. The resulting fusion products are thought to be components of the multimolecular complexes of axon initial segments and nodes of Ranvier, and they may play a role in calcium-mediated responses. [provided by RefSeq, Oct 2010]