NFIB (NM_001190737) Human Tagged ORF Clone

CAT#: RC231275

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NFIB (Myc-DDK-tagged)-Human nuclear factor I/B (NFIB), transcript variant 1



Reconstitution Protocol

USD 686.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol NFIB
Synonyms CTF; HMGIC/NFIB; MACID; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB2; NFIB3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC231275 representing NM_001190737
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTATTCTCCCATCTGTCTCACTCAGGATGAATTTCACCCATTCATCGAGGCACTTCTTCCACATG
TCCGTGCAATTGCCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAA
GCGAATGTCAAAGGATGAAGAAAGAGCAGTCAAAGATGAGCTTCTCAGTGAAAAGCCTGAAATCAAACAG
AAGTGGGCATCCAGGCTCCTTGCCAAACTGCGCAAAGATATTCGCCAGGAGTATCGAGAGGACTTTGTGC
TCACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCAATCCCGACCAGAAGGGTAAGATTAGGAG
AATCGACTGCCTGCGACAGGCAGACAAAGTCTGGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGC
ATCCCCTTGGAAAGTACCGATGGAGAGCGGCTCATGAAATCCCCACATTGCACAAACCCAGCACTTTGTG
TCCAGCCACATCATATCACAGTATCAGTTAAGGAGCTTGATTTGTTTTTGGCATACTACGTGCAGGAGCA
AGATTCTGGACAATCAGGAAGTCCAAGCCACAATGATCCTGCCAAGAATCCTCCAGGTTACCTTGAGGAT
AGTTTTGTAAAATCTGGAGTCTTCAATGTATCAGAACTTGTAAGAGTATCCAGAACGCCCATAACCCAGG
GAACTGGAGTCAACTTCCCAATTGGAGAAATCCCAAGCCAACCATACTATCATGACATGAACTCGGGGGT
CAATCTTCAGAGGTCTCTGTCTTCTCCACCAAGCAGCAAAAGACCCAAAACTATATCCATAGATGAAAAT
ATGGAACCAAGTCCTACAGGAGACTTTTACCCCTCTCCAAGTTCACCAGCTGCTGGAAGTCGAACATGGC
ACGAAAGAGATCAAGATATGTCTTCTCCGACTACTATGAAGAAGCCTGAAAAGCCATTGTTCAGCTCTGC
ATCTCCACAGGATTCTTCCCCAAGACTGAGCACTTTCCCCCAGCACCACCATCCCGGAATACCTGGAGTT
GCACACAGTGTCATCTCAACTCGAACTCCACCTCCACCTTCACCGTTGCCATTTCCAACACAAGCTATCC
TTCCTCCAGCCCCATCGAGCTACTTTTCTCATCCAACAATCAGATATCCTCCCCACCTGAATCCTCAGGA
TACTCTGAAGAACTATGTACCTTCTTATGACCCATCCAGTCCACAAACCAGCCAGCCTAACGGCAGTGGT
CAAGTAGTAGGGAAAGTGCCTGGCCATTTCACTCCTGTCTTGGCACCCTCTCCCCATCCCAGTGCAGTGC
GACCTGTGACCCTGAGCATGACAGATACTAAACCCATCACTACATCCACTGAAGCCTACACAGCCTCAGG
CACATCTCAAGCCAATCGATATGTGGGACTAAGCCCAAGAGACCCATCCTTCCTACATCAGCAACAGTCC
TGGTACCTGGGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC231275 representing NM_001190737
Red=Cloning site Green=Tags(s)

MMYSPICLTQDEFHPFIEALLPHVRAIAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQ
KWASRLLAKLRKDIRQEYREDFVLTVTGKKHPCCVLSNPDQKGKIRRIDCLRQADKVWRLDLVMVILFKG
IPLESTDGERLMKSPHCTNPALCVQPHHITVSVKELDLFLAYYVQEQDSGQSGSPSHNDPAKNPPGYLED
SFVKSGVFNVSELVRVSRTPITQGTGVNFPIGEIPSQPYYHDMNSGVNLQRSLSSPPSSKRPKTISIDEN
MEPSPTGDFYPSPSSPAAGSRTWHERDQDMSSPTTMKKPEKPLFSSASPQDSSPRLSTFPQHHHPGIPGV
AHSVISTRTPPPPSPLPFPTQAILPPAPSSYFSHPTIRYPPHLNPQDTLKNYVPSYDPSSPQTSQPNGSG
QVVGKVPGHFTPVLAPSPHPSAVRPVTLSMTDTKPITTSTEAYTASGTSQANRYVGLSPRDPSFLHQQQS
WYLG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001190737
ORF Size 1482 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001190737.2
RefSeq ORF 1485 bp
Locus ID 4781
UniProt ID O00712
Cytogenetics 9p23-p22.3
Protein Families Druggable Genome, Transcription Factors
MW 55.6 kDa
Gene Summary Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.[UniProtKB/Swiss-Prot Function]
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