AKR1D1 (NM_001190906) Human Tagged ORF Clone

CAT#: RC231126

AKR1D1 (Myc-DDK-tagged)-Human aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY434125) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC231126 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKR1D1
• Synonyms: 3o5bred; CBAS2; SRD5B1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231126 representing NM_001190906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCTCAGTGCTGCAAGTCACCGCATACCTCTAAGTGATGGAAACAGCATTCCCATCATCGGACTTG
  GTACCTACTCAGAACCTAAATCGACCCCTAAGGGAGCCTGTGCAACATCGGTGAAGGTTGCTATTGACAC
  AGGGTACCGACATATTGATGGGGCCTACATCTACCAAAATGAACACGAAGTTGGGGAGGCCATCAGGGAG
  AAGATAGCAGAAGGAAAGGTGCGGAGGGAAGATATCTTCTACTGTGGAAAGCTATGGGCTACAAATCATG
  TCCCAGAGATGGTCCGCCCAACCCTGGAGAGGACACTCAGGGTCCTCCAGCTAGATTATGTGGATCTTTA
  CATCATTGAAGTACCCATGGCCTTTAAGCCAGGAGATGAAATATACCCTAGAGATGAGAATGGCAAATGG
  TTATATCACAAGTCAAATCTGTGTGCCACTTGGGAGGTTGAGTGCCATCCGTATTTCACCCAGCCAAAAC
  TCTTGAAATTTTGCCAACAACATGACATTGTCATTACTGCATATAGCCCTTTGGGGACCAGTAGGAATCC
  AATCTGGGTGAATGTTTCTTCTCCACCTTTGTTAAAGGATGCACTTCTAAACTCATTGGGGAAAAGGTAC
  AATAAGACAGCAGCTCAAATTGTTTTGCGTTTCAACATCCAGCGAGGGGTGGTTGTCATTCCTAAAAGCT
  TTAATCTTGAAAGGATCAAAGAAAATTTTCAGATCTTTGACTTTTCTCTCACTGAAGAAGAAATGAAGGA
  CATTGAAGCCTTGAATAAAAATGTCCGCTTTGTAGAATTGCTCATGTGGCGCGATCATCCTGAATACCCA
  TTTCATGATGAATAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231126 representing NM_001190906
  Red=Cloning site Green=Tags(s)
  
  MDLSAASHRIPLSDGNSIPIIGLGTYSEPKSTPKGACATSVKVAIDTGYRHIDGAYIYQNEHEVGEAIRE
  KIAEGKVRREDIFYCGKLWATNHVPEMVRPTLERTLRVLQLDYVDLYIIEVPMAFKPGDEIYPRDENGKW
  LYHKSNLCATWEVECHPYFTQPKLLKFCQQHDIVITAYSPLGTSRNPIWVNVSSPPLLKDALLNSLGKRY
  NKTAAQIVLRFNIQRGVVVIPKSFNLERIKENFQIFDFSLTEEEMKDIEALNKNVRFVELLMWRDHPEYP
  FHDEY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190906
• ORF Size: 855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190906.2
• RefSeq ORF: 858 bp
• Locus ID: 6718
• UniProt ID: P51857
• Cytogenetics: 7q33
• Protein Families: Druggable Genome
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways, Primary bile acid biosynthesis
• MW: 33.3 kDa
• Gene Summary: The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet. [provided by RefSeq, Jul 2010]