C1orf93 (FAM213B) (NM_001195738) Human Tagged ORF Clone

CAT#: RC231058

FAM213B (Myc-DDK-tagged)-Human chromosome 1 open reading frame 93 (C1orf93), transcript variant 4

Product Images

Circular map for RC231058

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1orf93
• Synonyms: C1orf93; FAM213B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231058 representing NM_001195738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGGAGCGGGGATCCAGGAGCGAGGAGCCGGGAGCGGGGAACAGGGAGTCGGGGAGCCGGGAAC
  CAGGGCTGGCAGCGGCCGCCATGAGCACGGTGGACCTTGCTCGCGTGGGCGCGTGCATCCTGAAGCATGC
  GGTGACCGGGGAGGCCGTGGAGCTGCGGAGCCTGTGGCGGGAGCACGCGTGCGTGGTGGCCGGGCTGCGG
  CGCTTCGGGTGCGTGGTGTGCCGCTGGATCGCCCAGGACCTCAGCAGCCTTGCTGGGCTCCTGGACCAAC
  ACGGCGTGCGCCTGGTGGGCGTAGGGCCCGAGGCCCTGGGTCTGCAGGAGTTCCTGGACGGCGACTACTT
  CGCGGGAGAGCTCTACCTGGATGAGAGCAAGCAGCTTTACAAGGAGCTAGGCTTCAAGCGGTACAACAGC
  CTGAGCATCCTCCCAGCAGCTCTGGGGAAGCCCGTGCGTGATGTGGCTGCCAAGGCCAAGGCTGTTGGCA
  TCCAGGGGAACTTGTCTGGGGACCTGCTGCAGAGCGGAGGGCTGCTGGTGGTCAGCAAAGAAGTCCCCAG
  GCGACTACGTCCCCAAGGAGCACATCCTGCAGGTCCTGGGCATCTCTGCGGAGGTCTGTGCCAGCGACCC
  GCCTCAGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231058 representing NM_001195738
  Red=Cloning site Green=Tags(s)
  
  MSRERGSRSEEPGAGNRESGSREPGLAAAAMSTVDLARVGACILKHAVTGEAVELRSLWREHACVVAGLR
  RFGCVVCRWIAQDLSSLAGLLDQHGVRLVGVGPEALGLQEFLDGDYFAGELYLDESKQLYKELGFKRYNS
  LSILPAALGKPVRDVAAKAKAVGIQGNLSGDLLQSGGLLVVSKEVPRRLRPQGAHPAGPGHLCGGLCQRP
  ASV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001195738
• ORF Size: 639 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195738.1, NP_001182667.3
• RefSeq ORF: 552 bp
• Locus ID: 127281
• UniProt ID: Q8TBF2
• Cytogenetics: 1p36.32
• MW: 23 kDa
• Gene Summary: Catalyzes the reduction of prostaglandin-ethanolamide H(2) (prostamide H(2)) to prostamide F(2alpha) with NADPH as proton donor. Also able to reduce prostaglandin H(2) to prostaglandin F(2alpha) (By similarity).[UniProtKB/Swiss-Prot Function]