DGCR8 (NM_001190326) Human Tagged ORF Clone

CAT#: RC230872

DGCR8 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 8 (DGCR8), transcript variant 2

Product Images

Circular map for RC230872

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGCR8
• Synonyms: C22orf12; DGCRK6; Gy1; pasha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230872 representing NM_001190326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACAGATGAGAGCCCCTCTCCGCTCCCGTGTGGGCCCGCAGGAGAAGCGGTGATGGAGAGCCGAG
  CTCGCCCCTTCCAAGCGCTGCCCCGTGAGCAGTCTCCACCACCTCCCCTGCAAACGTCCAGTGGTGCAGA
  GGTAATGGACGTTGGCTCTGGTGGTGATGGACAGTCCGAACTCCCTGCTGAGGACCCCTTCAACTTCTAC
  GGAGCTTCTCTTCTCTCCAAAGGATCCTTCTCTAAGGGCCGCCTCCTCATAGACCCGAACTGTAGTGGCC
  ACAGCCCGCGCACCGCCCGGCACGCACCTGCGGTCCGGAAGTTCTCCCCTGACCTTAAGTTGCTTAAGGA
  TGTAAAGATTAGCGTGAGCTTTACCGAGAGCTGCAGGAGTAAGGACAGGAAGGTGCTGTACACAGGAGCA
  GAGCGCGACGTGCGGGCGGAGTGCGGTCTGCTCCTTAGCCCTGTCAGTGGGGACGTGCATGCTTGTCCCT
  TTGGCGGGAGTGTTGGTGACGGGGTAGGCATAGGGGGTGAGAGTGCTGATAAGAAGGATGAGGAGAATGA
  GCTGGATCAGGAAAAGAGAGTGGAGTATGCAGTGCTCGATGAGTTAGAAGATTTTACTGACAATTTGGAG
  CTAGATGAAGAAGGAGCAGGCGGGTTCACGGCTAAAGCAATCGTTCAGAGAGACAGAGTGGATGAAGAGG
  CCTTGAATTTCCCCTACGAGGATGACTTTGACAACGATGTGGATGCTCTGCTGGAAGAAGGCCTTTGTGC
  CCCCAAAAAGAGGCGAACAGAGGAAAAATATGGCGGAGACAGCGACCATCCGTCCGATGGAGAGACAAGT
  GTGCAGCCGATGATGACCAAGATTAAAACAGTGCTCAAAAGTCGTGGCCGCCCACCTACAGAGCCGCTGC
  CCGACGGGTGGATCATGACATTCCATAACTCTGGAGTCCCGGTGTACCTACACAGAGAGTCTCGGGTGGT
  CACCTGGTCCAGGCCATACTTCTTGGGAACGGGAAGCATACGGAAACACGACCCTCCTCTGAGTAGCATC
  CCTTGTCTGCATTATAAGAAAATGAAGGACAACGAGGAACGGGAGCAAAGCAGTGACCTCACCCCTAGTG
  GGGATGTGTCCCCCGTCAAGCCCCTGAGCCGATCTGCAGAGCTGGAGTTTCCCCTGGATGAGCCTGACTC
  TATGGGTGCTGACCCGGGGCCCCCGGACGAGAAAGACCCACTAGGGGCTGAGGCAGCCCCTGGGGCCCTG
  GGGCAGGTGAAGGCCAAAGTCGAGGTGTGCAAAGATGAATCCGTTGATCTCGAGGAATTTCGAAGCTACC
  TGGAGAAGCGTTTTGACTTTGAGCAAGTTACTGTGAAAAAATTCAGGACTTGGGCTGAGCGGCGGCAATT
  CAATCGGGAAATGAAGCGGAAGCAGGCGGAGTCCGAGAGGCCCATCTTGCCAGCCAATCAGAAGCTCATT
  ACTTTATCAGTGCAAGATGCACCCACAAAGAAAGAGTTTGTTATTAACCCCAACGGGAAATCCGAGGTCT
  GCATCCTGCACGAGTACATGCAGCGTGTCCTCAAGGTCCGCCCTGTCTATAATTTCTTTGAATGTGCCCG
  AGCTACACTGGAAATCCTCATCCCTGACTTTGTTAAACAGACCTCTGAAGAGAAGCCCAAAGACAGTGAA
  GAACTCGAGTATTTTAACCACATCAGCATCGAGGACTCGCGGGTCTACGAGCTGACCAGCAAGGCTGGGC
  TGTTGTCTCCATATCAGATCCTCCACGAGTGCCTTAAAAGAAACCATGGGATGGGTGACACGTCTATCAA
  GTTTGAAGTGGTTCCTGGGAAAAACCAGAAGAGTGAATACGTCATGGCGTGTGGCAAGCACACAGTGCGC
  GGGTGGTGTAAGAACAAGAGAGTTGGAAAGCAGTTAGCCTCACAGAAGATCCTTCAGCTGCTGCACCCAC
  ATGTCAAGAACTGGGGGTCTTTACTGCGCATGTATGGCCGTGAGAGCAGCAAGATGGTCAAGCAGGAGAC
  ATCGGACAAGAGTGTGATTGAGCTGCAGCAGTATGCCAAGAAGAACAAGCCCAACCTGCACATCCTCAGC
  AAGCTCCAAGAGGAGATGAAGAGGCTAGCTGAGGAAAGGGAGGAGACTCGAAAGAAGCCCAAGATGTCCA
  TTGTGGCGTCCGCCCAGCCTGGCGGTGAGCCCCTGTGCACCGTGGACGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230872 representing NM_001190326
  Red=Cloning site Green=Tags(s)
  
  METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFY
  GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGA
  ERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLE
  LDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETS
  VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI
  PCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGAL
  GQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLI
  TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECARATLEILIPDFVKQTSEEKPKDSE
  ELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVR
  GWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILS
  KLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001190326
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190326.1, NP_001177255.1
• RefSeq ORF: 2223 bp
• Locus ID: 54487
• UniProt ID: Q8WYQ5
• Cytogenetics: 22q11.21
• MW: 83.2 kDa
• Gene Summary: This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]