ZFAT (NM_001174158) Human Tagged ORF Clone

CAT#: RC230658

ZFAT (Myc-DDK-tagged)-Human zinc finger and AT hook domain containing (ZFAT), transcript variant 5

Product Images

Circular map for RC230658

• 

  Western blot validation of overexpression lysate (Cat# LY433657) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230658 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZFAT
• Synonyms: AITD3; ZFAT1; ZNF406
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230658 representing NM_001174158
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTAAATGTTGTAACCTCTTCTCACCAAATCAGTCGGAACTCCTCTCCCACGTTTCAGAGAAGCACA
  TGGAAGAAGGGGTTAATGTTGATGAGATTATTATTCCCCTTAGGCCTCTGAGTACACCTGAACCCCCCAA
  CTCAAGCAAAACCGGAGATGAGTTTTTGGTCATGAAGAGGAAGAGAGGCAGGCCTAAGGGGTCCACGAAG
  AAGTCCAGCACAGAAGAGGAGCTGGCAGAAAACATCGTGAGTCCGACTGAGGACAGCCCGCTGGCTCCGG
  AGGAAGGGAACAGCCTGCCTCCAAGCAGCTTGGAGTGTAGCAAGTGCTGTCGGAAGTTCTCCAACACGCG
  CCAGCTGCGGAAGCACATCTGCATTATCGTGCTGAATTTGGGTGAGGAGGAAGGAGAAGCAGGTAACGAG
  TCTGACCTTGAACTAGAAAAGAAGTGTAAGGAAGATGATCGGGAAAAAGCCTCGAAAAGACCACGGTCAC
  AGAAAACAGAGAAAGTCCAGAAGATCTCAGGAAAGGAGGCCAGACAGCTTTCTGGGGCGAAGAAACCCAT
  CATAAGTGTGGTTTTAACTGCACACGAAGCAATTCCAGGTGCTACCAAGATTGTGCCAGTGGAGGCTGGG
  CCCCCTGAAACAGGAGCTACAAATTCTGAGACCACTTCAGCAGACCTGGTGCCTCGGAGAGGCTACCAGG
  AATACGCCATTCAGCAGACACCTTATGAGCAACCAATGAAGTCAAGCAGGCTAGGTCCCACTCAGCTCAA
  AATCTTCACTTGTGAATACTGCAACAAGGTCTTCAAGTTCAAGCACTCGCTGCAGGCCCACCTGAGGATC
  CACACCAATGAAAAGCCATACAAGTGCCCCCAGTGCAGCTATGCCAGTGCCATCAAGGCCAACCTCAATG
  TGCACCTGCGCAAGCACACTGGAGAGAAGTTCGCCTGCGACTATTGCTCGTTCACCTGCCTGAGCAAGGG
  CCACCTCAAGGTGCACATCGAGCGAGTGCACAAGAAGATCAAGCAGCACTGCCGCTTCTGCAAGAAGAAG
  TACTCTGACGTCAAGAACCTCATCAAGCACATCCGAGACGCGCATGACCCACAGGACAAGAAGGTCAAAG
  AGGCCTTGGACGAGCTCTGCCTGATGACGAGGGAGGGCAAGCGGCAGCTGCTCTATGACTGCCACATCTG
  TGAGCGCAAGTTCAAGAACGAGCTGGACCGTGACCGCCATATGCTGGTCCACGGAGACAAGTGGCCTTTT
  GCCTGTGAGCTCTGTGGCCATGGGGCCACCAAGTACCAGGCGCTGGAACTGCATGTCAGGAAGCACCCCT
  TCGTGTACGTCTGTGCCGTCTGCCGCAAGAAGTTCGTCAGCTCCATCAGGCTGCGCACCCACATCAAAGA
  GGTGCACGGGGCTGCCCAGGAGGCCTTGGTCTTCACCAGTTCCATCAACCAGAGCTTCTGCCTCCTGGAA
  CCTGGTGGGGACATCCAGCAAGAAGCTCTGGGGGACCAGCTACAGCTGGTGGAAGAGGAGTTTGCCCTCC
  AGGGCGTGAATGCACTCAAGGAAGAGGCCTGTCCTGGGGACACTCAGCTGGAGGAGGGCCGGAAGGAGCC
  GGAGGCCCCTGGGGAAATGCCTGCCCCAGCTGTGCACCTGGCCTCCCCGCAGGCCGAAAGCACAGCCCTG
  CCACCCTGTGAGCTGGAAACCACCGTGGTCTCCTCCTCAGACCTGCATTCTCAAGAGGTGGTTTCAGATG
  ATTTTTTGTTGAAAAATGATACCTCCTCCGCAGAGGCTCATGCTGCTCCTGAGAAGCCCCCAGACATGCA
  GCACAGAAGCTCAGTCCAGACGCAAGGTGAAGTGATCACACTACTGCTGTCCAAGGCCCAGAGTGCTGGG
  TCAGATCAGGAAAGCCATGGCGCCCAGAGCCCCCTAGGGGAAGGGCAGAACATGGCTGTGCTTTCAGCTG
  GTGACCCAGATCCCAGCAGGTGTCTCAGGTCAAACCCAGCTGAGGCCTCAGACCTCCTCCCTCCAGTAGC
  TGGTGGTGGGGACACCATCACACATCAGCCTGACTCTTGCAAAGCTGCCCCTGAGCACCGGTCAGGCATC
  ACCGCTTTCATGAAGGTCCTGAACAGTTTACAGAAGAAGCAAATGAACACCAGCTTGTGTGAGCGGATCC
  GGAAGGTTTATGGAGACCTGGAGTGTGAATACTGTGGCAAACTTTTTTGGTACCAAGTGCATTTTGATAT
  GCATGTCCGCACCCACACCCGGGAACATCTGTATTATTGCTCTCAGTGTCATTATTCTTCCATCACCAAA
  AACTGCCTTAAACGCCACGTAATTCAGAAACACAGTAACATCTTGCTGAAGTGTCCCACCGATGGCTGTG
  ACTACTCAACTCCAGATAAATATAAGCTACAGGCACATCTTAAAGTTCACACAGCACTGGACAAAAGGAG
  TTATTCTTGTCCTGTTTGTGAAAAGTCTTTTTCAGAGGATCGATTGATAAAGTCACATATCAAGACCAAC
  CATCCTGAGGTCTCCATGAGCACCATTTCTGAGGTTCTCGGGAGGAGGGTTCAGCTGAAAGGGCTAATTG
  GAAAGAGAGCCATGAAATGCCCATATTGTGACTTTTATTTCATGAAGAATGGCTCAGACCTTCAGCGTCA
  TATTTGGGCTCATGAAGGTGTGAAGCCCTTCAAGTGTTCTTTGTGTGAGTATGCAACTCGTAGCAAGAGT
  AACCTCAAGGCTCATATGAATCGTCACAGCACTGAGAAAACCCACCTATGTGACATGTGTGGCAAGAAAT
  TCAAATCAAAAGGGACACTGAAAAGTCACAAACTCCTTCACACTGCAGATGGGAAGCAGTTTAAGTGCAC
  GGTGTGTGACTACACAGCGGCCCAGAAGCCACAGCTGCTGCGGCACATGGAACAGCATGTCTCCTTCAAG
  CCTTTCCGCTGTGCCCATTGCCATTACTCCTGCAACATATCTGGCTCTCTGAAGCGGCACTACAACAGGA
  AGCACCCTAATGAGGAGTATGCCAACGTGGGCACCGGGGAGCTGGCAGCGGAGGTGCTCATCCAGCAAGG
  TGGTTTGAAGTGTCCTGTTTGCAGCTTTGTATATGGCACCAAATGGGAGTTCAATAGGCACTTGAAGAAC
  AAACATGGCTTGAAGGTGGTGGAAATTGATGGAGACCCCAAGTGGGAGGTCACCGAGGAGGAGCCCAGCT
  CCAACCACACGGTCATGATCCAGGAGACGGTCCAGCAAGCGTCCGTGGAGCTTGCCGAGCAGCACCACCT
  GGTGGTGTCCTCCGACGACGTGGAGGGCATTGAGACGGTGACTGTCTACACGCAGGGCGGGGAGGCCTCG
  GAGTTCATCGTCTACGTGCAGGAGGCCATGCAGCCTGTGGAGGAGCAGGCTGTGGAGCAGCCGGCCCAGG
  AACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230658 representing NM_001174158
  Red=Cloning site Green=Tags(s)
  
  MCKCCNLFSPNQSELLSHVSEKHMEEGVNVDEIIIPLRPLSTPEPPNSSKTGDEFLVMKRKRGRPKGSTK
  KSSTEEELAENIVSPTEDSPLAPEEGNSLPPSSLECSKCCRKFSNTRQLRKHICIIVLNLGEEEGEAGNE
  SDLELEKKCKEDDREKASKRPRSQKTEKVQKISGKEARQLSGAKKPIISVVLTAHEAIPGATKIVPVEAG
  PPETGATNSETTSADLVPRRGYQEYAIQQTPYEQPMKSSRLGPTQLKIFTCEYCNKVFKFKHSLQAHLRI
  HTNEKPYKCPQCSYASAIKANLNVHLRKHTGEKFACDYCSFTCLSKGHLKVHIERVHKKIKQHCRFCKKK
  YSDVKNLIKHIRDAHDPQDKKVKEALDELCLMTREGKRQLLYDCHICERKFKNELDRDRHMLVHGDKWPF
  ACELCGHGATKYQALELHVRKHPFVYVCAVCRKKFVSSIRLRTHIKEVHGAAQEALVFTSSINQSFCLLE
  PGGDIQQEALGDQLQLVEEEFALQGVNALKEEACPGDTQLEEGRKEPEAPGEMPAPAVHLASPQAESTAL
  PPCELETTVVSSSDLHSQEVVSDDFLLKNDTSSAEAHAAPEKPPDMQHRSSVQTQGEVITLLLSKAQSAG
  SDQESHGAQSPLGEGQNMAVLSAGDPDPSRCLRSNPAEASDLLPPVAGGGDTITHQPDSCKAAPEHRSGI
  TAFMKVLNSLQKKQMNTSLCERIRKVYGDLECEYCGKLFWYQVHFDMHVRTHTREHLYYCSQCHYSSITK
  NCLKRHVIQKHSNILLKCPTDGCDYSTPDKYKLQAHLKVHTALDKRSYSCPVCEKSFSEDRLIKSHIKTN
  HPEVSMSTISEVLGRRVQLKGLIGKRAMKCPYCDFYFMKNGSDLQRHIWAHEGVKPFKCSLCEYATRSKS
  NLKAHMNRHSTEKTHLCDMCGKKFKSKGTLKSHKLLHTADGKQFKCTVCDYTAAQKPQLLRHMEQHVSFK
  PFRCAHCHYSCNISGSLKRHYNRKHPNEEYANVGTGELAAEVLIQQGGLKCPVCSFVYGTKWEFNRHLKN
  KHGLKVVEIDGDPKWEVTEEEPSSNHTVMIQETVQQASVELAEQHHLVVSSDDVEGIETVTVYTQGGEAS
  EFIVYVQEAMQPVEEQAVEQPAQEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001174158
• ORF Size: 3435 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174158.2
• RefSeq ORF: 3438 bp
• Locus ID: 57623
• Cytogenetics: 8q24.22
• MW: 129.1 kDa
• Gene Summary: This gene encodes a protein that likely binds DNA and functions as a transcriptional regulator involved in apoptosis and cell survival. This gene resides in a susceptibility locus for autoimmune thyroid disease (AITD) on chromosome 8q24. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Nov 2009]