RIM1 (RIMS1) (NM_001168408) Human Tagged ORF Clone

CAT#: RC230513

RIMS1 (Myc-DDK-tagged)-Human regulating synaptic membrane exocytosis 1 (RIMS1), transcript variant 3

Product Images

Circular map for RC230513

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIM1
• Synonyms: CORD7; RAB3IP2; RIM; RIM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230513 representing NM_001168408
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGTGCACCTGGGATTCATGTCTCTTCAGAAGGGTGGGAAGAAGTGAGGTCTGTTGATTCCGAAGAGG
  GAACAATTGAAGCTCGACGAGCAGTTGCTGGTGATTTGGATTATTACTGGTTGGATCCTGCCACGTGGCA
  CAGCCGGGAGACATCACCTATTAGTTCGCATCCTGTAACGTGGCAACCATCTAAAGAGGGGGACCGATTA
  ATTGGACGTGTTATTCTTAACAAGAGAACAACCATGCCCAAAGACTCAGGTGCATTGCTGGGTCTGAAAG
  TTGTTGGAGGAAAAATGACTGACTTAGGACGACTTGGTGCTTTCATCACCAAAGTAAAGAAGGGTAGCCT
  AGCAGATGTAGTTGGACACCTAAGAGCAGGGGATGAAGTTCTAGAATGGAATGGTAAACCCCTGCCGGGA
  GCTACAAATGAAGAAGTTTACAACATTATTTTAGAATCAAAATCAGAACCTCAAGTTGAAATTATTGTTT
  CAAGGCCTATTGGTGACATTCCCCGGATTCCTGAGAGCTCCCACCCTCCACTGGAGTCCAGTTCAAGTTC
  CTTTGAATCTCAGAAGATGGAAAGGCCTTCCATTTCTGTTATTTCTCCAACAAGTCCTGGAGCTCTAAAA
  GATGCCCCACAAGTCTTACCAGGGCAACTTTCTGTGAAGTTGTGGTATGATAAAGTGGGACACCAGCTGA
  TTGTAAATGTTCTGCAAGCAACAGATCTACCTGCTAGAGTAGATGGACGTCCTCGAAATCCCTATGTAAA
  AATGTATTTTCTTCCAGATAGAAGTGATAAAAGTAAAAGGAGGACCAAAACAGTAAAGAAAATACTAGAA
  CCAAAATGGAATCAAACTTTTGTCTATTCACATGTACATCGTAGAGATTTTAGAGAACGAATGTTAGAAA
  TAACTGTGTGGGACCAACCAAGAGTGCAAGAAGAAGAAAGTGAATTTCTTGGAGAGATCCTCATAGAATT
  GGAGACAGCGCTTTTAGATGATGAACCGCATTGGTATAAACTTCAGACACATGATGAGTCTTCACTACCT
  CTGCCTCAGCCATCACCTTTCATGCCAAGGCGACATATTCATGGAGAAAGCTCTAGCAAAAAGCTACAAA
  GATCTCAGCGAATCAGTGATAGTGACATCTCAGATTATGAGGTTGATGATGGTATTGGCGTAGTTCCTCC
  AGTAGGCTATAGGTCTAGTGCTAGAGAAAGTAAATCTACAACATTAACTGTGCCAGAACAGCAAAGAACA
  ACTCATCACCGCTCACGTTCAGTATCTCCTCATCGCGGCAATGATCAGGGAAAGCCGCGTTCACGTTTAC
  CAAATGTGCCATTACAGAGGAGTTTAGATGAAATTCATCCAACAAGAAGGTCACGTTCTCCAACCAGACA
  CCATGATGCCTCCCGAAGTCCAGTTGATCATAGAACCAGAGATGTGGATAGTCAGTATTTATCAGAACAA
  GACAGTGAGCTTCTTATGCTGCCCAGAGCAAAACGAGGACGAAGTGCAGAATGCCTACATACTACCAGTG
  AACTGCAGCCCTTTCTTGACAGGGCTAGGAGTGCTAGTACCAACTGCTTGAGACCAGATACTAGTTTGCA
  TTCACCAGAACGAGAAAGAATGCACCGACAGAGAAGTCCAACACAATCTCCTCCAGCAGACACATCGTTC
  AGCAGTCGCAGGGGAAGACAGCTCCCACAAGTGCCAGTGAGAAGCGGCAGTATAGAACAAGAGTCGGGCC
  ACAAAAAGTTAAAAAGTACCATCCAGAGAAGCACAGAAACAGGCATGGCAGCTGAAATGAGAAAGATGGT
  AAGGCAGCCGAGCCGAGAGTCTACTGATGGCAGCATCAACAGTTACAGCTCTGAGGGCAATTTAATATTT
  CCTGGAGTGCGACTGGGAGCTGACAGTCAATTCAGTGATTTTCTTGATGGATTGGGACCAGCCCAGCTTG
  TTGGCCGCCAAACCCTTGCCACCCCTGCAATGGGTGATATACAAATAGGAATGGAGGACAAAAAGGGCCA
  ATTAGAAGTGGAAGTCATTAGAGCACGAAGCCTCACACAAAAGCCTGGTTCCAAATCTACACCTGCTCCA
  TATGTCAAAGTATATCTTTTGGAAAATGGGGCCTGTATAGCCAAGAAGAAGACAAGAATTGCACGAAAAA
  CCCTTGATCCTTTGTATCAGCAGTCTCTGGTTTTTGATGAAAGTCCACAGGGTAAAGTTCTTCAGGTGAT
  TGTCTGGGGAGACTATGGCAGAATGGACCACAAATGCTTTATGGGTGTGGCTCAGATCTTGTTGGAAGAA
  CTCGACCTGTCCAGCATGGTGATCGGATGGTACAAATTGTTCCCACCGTCCTCACTGGTGGATCCCACAC
  TCACTCCCCTCACCCGGCGGGCTTCCCAGTCATCTCTGGAAAGTTCAACTGGGCCTCCCTGTATTCGATC
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230513 representing NM_001168408
  Red=Cloning site Green=Tags(s)
  
  MCAPGIHVSSEGWEEVRSVDSEEGTIEARRAVAGDLDYYWLDPATWHSRETSPISSHPVTWQPSKEGDRL
  IGRVILNKRTTMPKDSGALLGLKVVGGKMTDLGRLGAFITKVKKGSLADVVGHLRAGDEVLEWNGKPLPG
  ATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHPPLESSSSSFESQKMERPSISVISPTSPGALK
  DAPQVLPGQLSVKLWYDKVGHQLIVNVLQATDLPARVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKILE
  PKWNQTFVYSHVHRRDFRERMLEITVWDQPRVQEEESEFLGEILIELETALLDDEPHWYKLQTHDESSLP
  LPQPSPFMPRRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRSSARESKSTTLTVPEQQRT
  THHRSRSVSPHRGNDQGKPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSPVDHRTRDVDSQYLSEQ
  DSELLMLPRAKRGRSAECLHTTSELQPFLDRARSASTNCLRPDTSLHSPERERMHRQRSPTQSPPADTSF
  SSRRGRQLPQVPVRSGSIEQESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRESTDGSINSYSSEGNLIF
  PGVRLGADSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDKKGQLEVEVIRARSLTQKPGSKSTPAP
  YVKVYLLENGACIAKKKTRIARKTLDPLYQQSLVFDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEE
  LDLSSMVIGWYKLFPPSSLVDPTLTPLTRRASQSSLESSTGPPCIRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001168408
• ORF Size: 2451 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001168408.2
• RefSeq ORF: 2454 bp
• Locus ID: 22999
• UniProt ID: Q86UR5
• Cytogenetics: 6q13
• MW: 92 kDa
• Gene Summary: The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]