DPP10 (NM_001178037) Human Tagged ORF Clone

CAT#: RC230500

DPP10 (Myc-DDK-tagged)-Human dipeptidyl-peptidase 10 (non-functional) (DPP10), transcript variant 4

Product Images

Circular map for RC230500

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPP10
• Synonyms: DPL2; DPPY; DPRP-3; DPRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230500 representing NM_001178037
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCATCACAGAGGTGGCTGAGGGACCAGTAGATGGTTCTGAAAAGGAACTGGGAAGTAACAGCCCTC
  CACAGAGAAACTGGAAGGGAATTGCTATTGCTCTGCTGGTGATTTTAGTTGTATGCTCACTCATCACTAT
  GTCAGTCATCCTCTTAACCCCAGATGAACTCACAAATTCGTCAGAAACCAGATTGTCTTTGGAAGACCTC
  TTTAGGAAAGACTTTGTGCTTCACGATCCAGAGGCTCGGTGGATCAATGATACAGATGTGGTGTATAAAA
  GCGAGAATGGACATGTCATTAAACTGAATATAGAAACAAATGCTACCACATTATTATTGGAAAACACAAC
  TTTTGTAACCTTCAAAGCATCAAGACATTCAGTTTCACCAGATTTAAAATATGTCCTTCTGGCATATGAT
  GTCAAACAGATTTTTCATTATTCGTATACTGCTTCATATGTGATTTACAACATACACACTAGGGAAGTTT
  GGGAGTTAAATCCTCCAGAAGTAGAGGACTCCGTCTTGCAGTACGCGGCCTGGGGTGTCCAAGGGCAGCA
  GCTGATTTATATTTTTGAAAATAATATCTACTATCAACCTGATATAAAGAGCAGTTCATTGCGACTGACA
  TCTTCTGGAAAAGAAGAAATAATTTTTAATGGGATTGCTGACTGGTTATATGAAGAGGAACTCCTGCATT
  CTCACATCGCCCACTGGTGGTCACCAGATGGAGAAAGACTTGCCTTCCTGATGATAAATGACTCTTTGGT
  ACCCACCATGGTTATCCCTCGGTTTACTGGAGCGTTGTATCCCAAAGGAAAGCAGTATCCGTATCCTAAG
  GCAGGTCAAGTGAACCCAACAATAAAATTATATGTTGTAAACCTGTATGGACCAACTCACACTTTGGAGC
  TCATGCCACCTGACAGCTTTAAATCAAGAGAATACTATATCACTATGGTTAAATGGGTAAGCAATACCAA
  GACTGTGGTAAGATGGTTAAACCGAGCTCAGAACATCTCCATCCTCACAGTCTGTGAGACCACTACAGGT
  GCTTGTAGTAAAAAATATGAGATGACATCAGATACGTGGCTCTCTCAGCAGAATGAGGAGCCCGTGTTTT
  CTAGAGACGGCAGCAAATTCTTTATGACAGTGCCTGTTAAGCAAGGGGGACGTGGAGAATTTCACCACAT
  AGCTATGTTCCTCATCCAGAGTAAAAGTGAGCAAATTACCGTGCGGCATCTGACATCAGGAAACTGGGAA
  GTGATAAAGATCTTGGCATACGATGAAACTACTCAAAAAATTTACTTTCTGAGCACTGAATCTTCTCCCA
  GAGGAAGGCAGCTGTACAGTGCTTCTACTGAAGGATTATTGAATCGCCAATGCATTTCATGTAATTTCAT
  GAAAGAACAATGTACATATTTTGATGCCAGTTTTAGTCCCATGAATCAACATTTCTTATTATTCTGTGAA
  GGTCCAAGGGTCCCAGTGGTCAGCCTACATAGTACGGACAACCCAGCAAAATATTTTATATTGGAAAGCA
  ATTCTATGCTGAAGGAAGCTATCCTGAAGAAGAAGATAGGAAAGCCAGAAATTAAAATCCTTCATATTGA
  CGACTATGAACTTCCTTTACAGTTGTCCCTTCCCAAAGATTTTATGGACCGAAACCAGTATGCTCTTCTG
  TTAATAATGGATGAAGAACCAGGAGGCCAGCTGGTTACAGATAAGTTCCATATTGACTGGGATTCCGTAC
  TCATTGACATGGATAATGTCATTGTAGCAAGATTTGATGGCAGAGGAAGTGGATTCCAGGGTCTGAAAAT
  TTTGCAGGAGATTCATCGAAGATTAGGTTCAGTAGAAGTAAAGGACCAAATAACAGCTGTGAAATTTTTG
  CTGAAACTGCCTTACATTGACTCCAAAAGATTAAGCATTTTTGGAAAGGGTTATGGTGGCTATATTGCAT
  CAATGATCTTAAAATCAGATGAAAAGCTTTTTAAATGTGGATCCGTGGTTGCACCTATCACAGACTTGAA
  ATTGTATGCCTCAGCTTTCTCTGAAAGATACCTTGGGATGCCATCTAAGGAAGAAAGCACTTACCAGGCA
  GCCAGTGTGCTACATAATGTTCATGGCTTGAAAGAAGAAAATATATTAATAATTCATGGAACTGCTGACA
  CAAAAGTTCATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAGCTGGAGTGAATTATACTAT
  GCAGGTCTACCCAGATGAAGGTCATAACGTATCTGAGAAGAGCAAGTATCATCTCTACAGCACAATCCTC
  AAATTCTTCAGTGATTGTTTGAAGGAAGAAATATCTGTGCTACCACAGGAACCAGAAGAAGATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230500 representing NM_001178037
  Red=Cloning site Green=Tags(s)
  
  MVITEVAEGPVDGSEKELGSNSPPQRNWKGIAIALLVILVVCSLITMSVILLTPDELTNSSETRLSLEDL
  FRKDFVLHDPEARWINDTDVVYKSENGHVIKLNIETNATTLLLENTTFVTFKASRHSVSPDLKYVLLAYD
  VKQIFHYSYTASYVIYNIHTREVWELNPPEVEDSVLQYAAWGVQGQQLIYIFENNIYYQPDIKSSSLRLT
  SSGKEEIIFNGIADWLYEEELLHSHIAHWWSPDGERLAFLMINDSLVPTMVIPRFTGALYPKGKQYPYPK
  AGQVNPTIKLYVVNLYGPTHTLELMPPDSFKSREYYITMVKWVSNTKTVVRWLNRAQNISILTVCETTTG
  ACSKKYEMTSDTWLSQQNEEPVFSRDGSKFFMTVPVKQGGRGEFHHIAMFLIQSKSEQITVRHLTSGNWE
  VIKILAYDETTQKIYFLSTESSPRGRQLYSASTEGLLNRQCISCNFMKEQCTYFDASFSPMNQHFLLFCE
  GPRVPVVSLHSTDNPAKYFILESNSMLKEAILKKKIGKPEIKILHIDDYELPLQLSLPKDFMDRNQYALL
  LIMDEEPGGQLVTDKFHIDWDSVLIDMDNVIVARFDGRGSGFQGLKILQEIHRRLGSVEVKDQITAVKFL
  LKLPYIDSKRLSIFGKGYGGYIASMILKSDEKLFKCGSVVAPITDLKLYASAFSERYLGMPSKEESTYQA
  ASVLHNVHGLKEENILIIHGTADTKVHFQHSAELIKHLIKAGVNYTMQVYPDEGHNVSEKSKYHLYSTIL
  KFFSDCLKEEISVLPQEPEEDE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001178037
• ORF Size: 2376 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001178037.1, NP_001171508.1
• RefSeq ORF: 2379 bp
• Locus ID: 57628
• Cytogenetics: 2q14.1
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 90.8 kDa
• Gene Summary: This gene encodes a single-pass type II membrane protein that is a member of the S9B family in clan SC of the serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Mutations in this gene have been associated with asthma. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]