SEC23B (NM_001172746) Human Tagged ORF Clone

CAT#: RC230476

SEC23B (Myc-DDK-tagged)-Human Sec23 homolog B (S. cerevisiae) (SEC23B), transcript variant 5

Product Images

Circular map for RC230476

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEC23B
• Synonyms: CDA-II; CDAII; CDAN2; CWS7; HEMPAS; hSec23B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230476 representing NM_001172746
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACATACCTGGAGTTCATCCAGCAGAATGAAGAACGGGATGGTGTGCGTTTTAGTTGGAACGTGT
  GGCCTTCCAGCCGGCTGGAGGCTACAAGAATGGTTGTACCCCTGGCTTGTCTCCTTACTCCTTTGAAAGA
  ACGTCCAGACCTACCTCCTGTACAATATGAACCTGTGCTTTGCAGCAGGCCAACTTGTAAAGCTGTTCTC
  AACCCACTTTGTCAGGTTGATTATCGAGCAAAACTTTGGGCCTGTAATTTCTGTTTTCAAAGAAATCAGT
  TTCCTCCAGCTTATGGAGGCATATCTGAGGTGAATCAACCTGCCGAATTGATGCCCCAGTTTTCTACAAT
  TGAGTACGTGATACAGGAAGATGACCTTCAAGCACTCAAAGAGTCCCTGCAGATGTCCCTGAGTCTTCTT
  CCTCCAGATGCTCTGGTGGGTCTGATCACATTTGGAAGGATGGTGCAGGTTCATGAGCTAAGCTGTGAAG
  GAATCTCCAAAAGTTATGTCTTCCGAGGGACCAAGGATTTAACTGCAAAGCAAATACAGGATATGTTGGG
  CCTGACCAAGCCAGCCATGCCCATGCAGCAAGCACGACCTGCACAACCACAGGAGCACCCTTTTGCTTCA
  AGCAGATTTCTGCAGCCTGTTCACAAGATTGATATGAACCTCACTGATCTTCTTGGGGAGCTACAGAGGG
  ACCCATGGCCAGTAACTCAGGGGAAGAGACCTTTGCGATCCACTGGTGTGGCTTTGTCCATTGCTGTTGG
  CTTGCTGGAGGGCACTTTTCCAAACACAGGAGCCAGGATCATGCTGTTTACTGGAGGTCCCCCTACCCAA
  GGGCCTGGCATGGTGGTTGGAGATGAATTAAAGATTCCTATTCGTTCTTGGCATGATATTGAGAAAGATA
  ATGCACGATTCATGAAAAAGGCAACCAAGCACTATGAGATGCTTGCTAATCGAACAGCTGCAAATGGTCA
  CTGCATTGATATTTATGCTTGTGCCCTTGATCAAACTGGACTTTTGGAGATGAAGTGTTGTGCAAATCTT
  ACTGGAGGCTACATGGTAATGGGAGATTCTTTCAACACTTCTCTCTTCAAGCAGACATTCCAAAGAATCT
  TTACTAAAGATTTTAATGGAGATTTCCGAATGGCATTTGGTGCTACTTTGGACGTAAAGACCTCTCGGGA
  ACTGAAGATTGCAGGAGCCATTGGTCCATGCGTATCTCTGAATGTGAAAGGACCGTGTGTGTCAGAAAAT
  GAGCTTGGTGTTGGTGGCACGAGTCAGTGGAAAATCTGTGGCCTAGATCCTACATCTACACTTGGCATCT
  ATTTTGAAGTTGTCAATCAGCACAACACCCCGATCCCCCAAGGAGGCAGAGGAGCCATCCAGTTTGTCAC
  GCATTATCAGCACTCCAGCACCCAGAGACGCATCCGCGTGACCACCATCGCCCGAAATTGGGCAGATGTA
  CAGAGTCAGCTCAGGCACATAGAAGCAGCATTTGACCAGGAGGCTGCGGCAGTGTTGATGGCACGGCTTG
  GGGTGTTCCGAGCGGAGTCAGAGGAGGGGCCCGATGTGCTCCGGTGGCTGGACCGACAACTCATCCGACT
  GTGTCAAAAGTTTGGACAGTATAACAAAGAAGACCCCACTTCTTTTAGGTTATCAGATTCCTTTTCTCTA
  TATCCTCAGTTTATGTTCCATCTGAGAAGATCTCCATTTCTTCAAGTGTTTAACAACAGTCCTGATGAGT
  CGTCATATTACAGACATCATTTTGCCCGGCAGGACCTGACCCAGTCCCTCATCATGATCCAGCCCATTCT
  CTACTCTTACTCCTTTCATGGGCCACCAGAGCCAGTACTCTTGGATAGCAGCAGCATTCTAGCTGACAGA
  ATTTTGCTGATGGATACTTTCTTTCAAATTGTCATTTATCTTGGTGAGACCATAGCCCAGTGGCGTAAAG
  CTGGCTACCAGGACATGCCCGAGTATGAAAACTTCAAGCACCTTCTGCAGGCACCACTGGATGATGCTCA
  AGAAATTCTGCAAGCACGCTTCCCGATGCCACGTTACATCAACACGGAGCATGGAGGCAGTCAGGCTCGA
  TTCCTTTTGTCCAAAGTGAACCCATCTCAGACACACAATAACCTGTATGCTTGGGGACAGGAAACTGGAG
  CACCCATCCTAACTGATGATGTTAGCCTGCAGGTGTTCATGGACCATTTGAAGAAGCTGGCTGTCTCCAG
  TGCCTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230476 representing NM_001172746
  Red=Cloning site Green=Tags(s)
  
  MATYLEFIQQNEERDGVRFSWNVWPSSRLEATRMVVPLACLLTPLKERPDLPPVQYEPVLCSRPTCKAVL
  NPLCQVDYRAKLWACNFCFQRNQFPPAYGGISEVNQPAELMPQFSTIEYVIQEDDLQALKESLQMSLSLL
  PPDALVGLITFGRMVQVHELSCEGISKSYVFRGTKDLTAKQIQDMLGLTKPAMPMQQARPAQPQEHPFAS
  SRFLQPVHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGVALSIAVGLLEGTFPNTGARIMLFTGGPPTQ
  GPGMVVGDELKIPIRSWHDIEKDNARFMKKATKHYEMLANRTAANGHCIDIYACALDQTGLLEMKCCANL
  TGGYMVMGDSFNTSLFKQTFQRIFTKDFNGDFRMAFGATLDVKTSRELKIAGAIGPCVSLNVKGPCVSEN
  ELGVGGTSQWKICGLDPTSTLGIYFEVVNQHNTPIPQGGRGAIQFVTHYQHSSTQRRIRVTTIARNWADV
  QSQLRHIEAAFDQEAAAVLMARLGVFRAESEEGPDVLRWLDRQLIRLCQKFGQYNKEDPTSFRLSDSFSL
  YPQFMFHLRRSPFLQVFNNSPDESSYYRHHFARQDLTQSLIMIQPILYSYSFHGPPEPVLLDSSSILADR
  ILLMDTFFQIVIYLGETIAQWRKAGYQDMPEYENFKHLLQAPLDDAQEILQARFPMPRYINTEHGGSQAR
  FLLSKVNPSQTHNNLYAWGQETGAPILTDDVSLQVFMDHLKKLAVSSAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172746
• ORF Size: 2247 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172746.3
• RefSeq ORF: 2250 bp
• Locus ID: 10483
• Cytogenetics: 20p11.23
• MW: 84.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the SEC23 subfamily of the SEC23/SEC24 family, which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec23p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. The function of this gene product has been implicated in cargo selection and concentration. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]