DPY19L3 (NM_001172774) Human Tagged ORF Clone

CAT#: RC230454

DPY19L3 (Myc-DDK-tagged)-Human dpy-19-like 3 (C. elegans) (DPY19L3), transcript variant 2

Product Images

Circular map for RC230454

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DPY19L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC230454 representing NM_001172774
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCCATCCGGCAAAGAAGAGAAATAAGAGCCACAGAAGTTTCTGAAGACTTTCCAGCCCAAGAAG
  AAAATGTGAAGTTGGAAAATAAATTGCCATCTGGTTGTACCAGTAGAAGATTATGGAAGATTTTGTCATT
  GACAATTGGTGGAACCATTGCCCTTTGCATTGGACTTCTTACATCTGTCTACCTTGCCACGTTACATGAA
  AATGATTTATGGTTTTCTAATATTAAGGAAGTGGAGCGAGAAATCTCATTCAGAACAGAGTGTGGCCTGT
  ATTACTCCTACTACAAGCAGATGCTGCAGGCTCCAACCCTCGTGCAAGGTTTTCATGGCCTAATATATGA
  TAATAAAACTGAATCTATGAAGACAATTAACCTCCTTCAGCGAATGAATATTTACCAAGAGGTTTTTCTC
  AGTATTTTATATAGAGTTCTACCCATACAGAAATATTTAGAGCCAGTTTATTTTTATATTTACACCTTAT
  TTGGGCTCCAGGCGATCTATGTCACAGCTCTCTACATAACCAGCTGGCTACTCAGTGGTACATGGCTGTC
  AGGACTGTTGGCAGCTTTCTGGTATGTCACAAATAGAATAGATACCACAAGAGTTGAGTTTACCATCCCA
  CTGAGGGAGAACTGGGCACTGCCATTCTTTGCAATTCAGATAGCAGCAATTACATATTTCCTGAGACCAA
  ACTTACAGCCTCTTTCTGAAAGGCTGACACTTCTTGCCATTTTCATATCAACTTTTCTCTTTAGTCTGAC
  ATGGCAATTTAATCAATTTATGATGCTGATGCAAGCATTAGTGCTGTTCACACTGGACTCCCTGGACATG
  CTGCCAGCAGTGAAGGCGACATGGCTGTATGGAATACAGATAACAAGTTTACTCCTGGTCTGCATTCTTC
  AGTTTTTTAATTCCATGATTCTTGGATCACTGCTTATCAGTTTTAACCTTTCAGTATTCATTGCAAGAAA
  ACTTCAGAAAAATCTGAAAACTGGAAGCTTCCTTAATAGGCTTGGGAAACTTTTGTTACATTTATTTATG
  GTTTTATGTTTGACACTTTTTCTCAACAACATAATTAAGAAAATTCTTAACCTGAAGTCAGATGAACACA
  TATTTAAATTTCTGAAGGCAAAATTTGGGCTTGGAGCAACAAGGGATTTTGATGCAAATCTCTATCTGTG
  TGAAGAAGCTTTTGGCCTCCTGCCTTTTAATACATTTGGAAGGCTTTCAGATACTCTGCTTTTTTATGCT
  TACATATTCGTTCTGTCCATCACAGTGATTGTAGCATTCGTTGTTGCCTTTCATAATCTCAGTGATTCTA
  CAAATCAACAATCCGTGGGTAAAATGGAAAAAGGCACAGTTGACCTGAAACCAGAAACTGCCTACAACTT
  AATACATACCATTCTGTTTGGATTCTTGGCATTGAGTACAATGAGAATGAAGTACCTCTGGACGTCACAC
  ATGTGTGTGTTCGCATCATTCGGCCTATGTAGCCCTGAAATATGGGAGTTACTTCTGAAGTCAGTCCATC
  TTTATAACCCAAAGAGGATATGTATAATGCGATATTCAGTACCGATATTAATACTGCTGTATCTATGCTA
  TAAGTTCTGGCCAGGAATGATGGATGAACTCTCCGAGTTGAGAGAATTCTATGATCCAGATACAGTGGAG
  CTGATGAACTGGATTAACTCTAACACTCCAAGAAAGGCTGTGTTTGCGGGAAGCATGCAGTTGCTGGCCG
  GAGTCAAGCTGTGCACGGGAAGGACCCTAACCAACCACCCGCACTATGAAGACAGCAGCCTGAGAGAGCG
  GACCAGAGCGGTTTATCAGATATATGCCAAGAGGGCACCAGAGGAAGTGCATGCCCTCCTAAGGTCCTTC
  GGCACTGACTACGTAATCCTGGAAGACAGCATCTGCTACGAGCGGAGGCACCGCCGGGGCTGCCGACTCC
  GGGACCTGCTGGACATTGCCAACGGCCACATGATGGATGGCCCAGGAGAGAATGATCCTGATTTGAAACC
  TGCAGACCACCCTCGCTTCTGTGAAGAGATCAAAAGAAACCTGCCTCCCTACGTGGCCTACTTCACCAGA
  GTGTTCCAGAACAAAACCTTCCACGTTTACAAGCTGTCCAGAAACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC230454 representing NM_001172774
  Red=Cloning site Green=Tags(s)
  
  MMSIRQRREIRATEVSEDFPAQEENVKLENKLPSGCTSRRLWKILSLTIGGTIALCIGLLTSVYLATLHE
  NDLWFSNIKEVEREISFRTECGLYYSYYKQMLQAPTLVQGFHGLIYDNKTESMKTINLLQRMNIYQEVFL
  SILYRVLPIQKYLEPVYFYIYTLFGLQAIYVTALYITSWLLSGTWLSGLLAAFWYVTNRIDTTRVEFTIP
  LRENWALPFFAIQIAAITYFLRPNLQPLSERLTLLAIFISTFLFSLTWQFNQFMMLMQALVLFTLDSLDM
  LPAVKATWLYGIQITSLLLVCILQFFNSMILGSLLISFNLSVFIARKLQKNLKTGSFLNRLGKLLLHLFM
  VLCLTLFLNNIIKKILNLKSDEHIFKFLKAKFGLGATRDFDANLYLCEEAFGLLPFNTFGRLSDTLLFYA
  YIFVLSITVIVAFVVAFHNLSDSTNQQSVGKMEKGTVDLKPETAYNLIHTILFGFLALSTMRMKYLWTSH
  MCVFASFGLCSPEIWELLLKSVHLYNPKRICIMRYSVPILILLYLCYKFWPGMMDELSELREFYDPDTVE
  LMNWINSNTPRKAVFAGSMQLLAGVKLCTGRTLTNHPHYEDSSLRERTRAVYQIYAKRAPEEVHALLRSF
  GTDYVILEDSICYERRHRRGCRLRDLLDIANGHMMDGPGENDPDLKPADHPRFCEEIKRNLPPYVAYFTR
  VFQNKTFHVYKLSRNK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172774
• ORF Size: 2148 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172774.1, NP_001166245.1
• RefSeq ORF: 2151 bp
• Locus ID: 147991
• UniProt ID: Q6ZPD9
• Cytogenetics: 19q13.11
• Protein Families: Transmembrane
• MW: 83.6 kDa
• Gene Summary: Probable C-mannosyltransferase that mediates C-mannosylation of tryptophan residues on target proteins.[UniProtKB/Swiss-Prot Function]